Irinotecan cpt 11

To determine the IC₅₀ values of irinotecan (CPT-11; LC Laboratories, Woburn, MA, USA) and LB-100 (Selleckchem, Houston, TX, USA), completely starved H1299 cells were seeded into 96-well plates at 3 × 10³ cells/well and incubated for 24 h in 10% FBS–RPMI 1640 medium. Subsequently, irinotecan or LB-100 was added at concentrations of 0–100 μM, and the cells were incubated for 48 h. Cell viability was investigated using the CCK-8 assay (Dojindo, Rockville, MD, USA).

Fe(III) chloride hexahydrate (FeCl₃·6H₂O; 98%), 2‐aminoterephthalic acid (BDC‐NH₂; 99%), N,N‐dimethylformamide (DMF; anhydrous, 99.8%), d‐penicillamine (d‐pen; 98%–101%), 4‐chloro‐7‐nitrobenzofurazan (NBD‐CL; 98%), copper(II) sulfate (CuSO₄; >99%), formic acid (>98%) and 2′‐7′‐dichlorofluorescein diacetate (DCFH‐DA) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Methanol (>96%), ethanol (>96%), ammonium acetate (98%), sodium borate (Na₂B₄O₇), and hydrogen peroxide (H₂O₂; 30%–35%) were obtained from Daejung Chemicals (Siheung, Korea). 3,3′,5,5′‐Tetramethylbenzidine (TMB; >98%) and acetonitrile (ACN, 99.9%) were purchased from TCI (Chuo‐ku, Japan) and J.T. Bakers (Pittsburgh, PA, USA), respectively. Irinotecan (CPT‐11; >99%) was obtained from LC Laboratories (Woburn, MA, USA). Phosphate‐buffered saline (PBS, pH 7.4) and Dulbecco's Modified Eagle's Medium (DMEM) were obtained from the Seoul National University Biomedical Research Institute (Seoul, Korea) and Welgene (Gyeongsan, Korea), respectively. Fetal bovine serum (FBS) and penicillin–streptomycin (10,000 U/ml) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).