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A549 crm ccl 185

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A549 (CRM-CCL-185) is an adherent human alveolar basal epithelial cell line derived from a 58-year-old Caucasian male with lung carcinoma. It is a well-characterized cell line commonly used in biomedical research.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) 293t crl 3216, by American Type Culture Collection (1 mentions) Beas 2b crl 9609, by American Type Culture Collection (1 mentions) Penicillin sodium, by Thermo Fisher Scientific (1 mentions) Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) H1975, by American Type Culture Collection (1 mentions) Glycine, by Merck Group (1 mentions) Mannitol, by Roquette (1 mentions) Thp 1 tib 202, by American Type Culture Collection (1 mentions) Vero e6 crl 1586, by American Type Culture Collection (1 mentions)

Spelling variants of this product

CCL-185 (217 citations) A549 (CCL-185) (64 citations) CCL-185TM (11 citations) CRM-CCL-185 (10 citations)

5 protocols using a549 crm ccl 185

1

Cell Culture of 293T and A549 Cells

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293T (CRL-3216) and A549 (CRM-CCL-185) cells were obtained from the American Type Culture Collection (ATCC) and were grown in Dulbecco’s modified Eagle medium (DMEM, Gibco) containing glutamine, supplemented with 10% fetal bovine serum (FBS). The cells were cultured at 37 °C in a humidified incubator with 5% CO2.
Tsang N.Y., Li W.F., Varhegyi E., Rong L, & Zhang H.J. (2022). Ebola Entry Inhibitors Discovered from Maesa perlarius. International Journal of Molecular Sciences, 23(5), 2620.
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2

Cell Culture of A549 and BEAS-2B

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A549 (CRM-CCL-185) and BEAS-2B (CRL-9609) cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and A549/DDP (GF452) cells were obtained from Gefan Biotechnology Co., Ltd. (Shanghai, China). The cells were maintained in RPMI-1640 medium (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Inc.) at 37°C in an atmosphere of 5% CO2.
Ren A., Wen Z, & Zheng L. (2019). Downregulation of miR-3934-5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1. Experimental and Therapeutic Medicine, 18(3), 1653-1660.
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3

Transfection of 293T and A549 Cells

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The 293T (CRL-11268™) and A549 (CRM-CCL-185) cells were purchased from American Type Culture Collection (VA, USA). Monolayer cells were cultivated in six-well plates in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal bovine serum and 1% penicillin and streptomycin in an incubator under 5% CO2 and 37 °C conditions. At 80% confluency, 5 μg L1 or L2 was added to each well for transfecting the cells using ER4000 transfection reagent, as per the manufacturer’s instructions (Engreen Biosystem, 4000-4, Beijing, China). The cells were then collected at relevant time points for subsequent experiments.
Liu D., An C., Bai Y., Li K., Liu J., Wang Q., He Q., Song Z., Zhang J., Song L., Cui B., Mao Q., Jiang W, & Liang Z. (2022). A Novel Single-Stranded RNA-Based Adjuvant Improves the Immunogenicity of the SARS-CoV-2 Recombinant Protein Vaccine. Viruses, 14(9), 1854.
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4

Maintaining Human Lung Carcinoma Cells

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Human lung carcinoma cell lines A549 (CRM-CCL-185) and H1975 (CRL-5908) were purchased from American Type Culture Collection (Manassas, VA). Cells were maintained in RPMI 1640 supplemented with 10% fetal bovine serum, 100 U/mL penicillin sodium, and 100 μg/mL streptomycin sulfate (Thermo Fisher Scientific; Waltham, MA) at 37°C in a humidified atmosphere with 5% CO2. The GFP/luciferase-expressing A549 cell line was generated after transduction with pCCLc-Luc-EGFP lentiviral constructs (Vector Core; UC Davis Medical Center, Sacramento, CA) [17 (link)]. Cells in the logarithmic growth phases were used for the experiments.
Petrek H., Ho P.Y., Batra N., Tu M.J., Zhang Q., Qiu J.X, & Yu A.M. (2021). Single bioengineered ncRNA molecule for dual-targeting toward the control of non-small cell lung cancer patient-derived xenograft tumor growth. Biochemical pharmacology, 189, 114392.
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5

Dry Powder Inhaler Formulation and Evaluation

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CsA (Metapharmaceutical, Barcelona, Spain) was purchased from ACEF (Fiorenzuola d’Arda, Italy). HPMC extra-dry capsules for use in dry powder inhalers, Quali-V®-I size #3, were provided by Qualicaps (Madrid, Spain), while the high-resistance dry powder inhaler RS01 was gifted by Plastiape (Lecco, Italy). Mannitol was purchased from Roquette (Lestrem, France) and glycine was purchased from Sigma Aldrich (Merck, Milano, Italy). All other chemicals used were obtained from commercial suppliers and were at least of analytical grade. The human lung adenocarcinoma cell line A549 (CRM-CCL-185), monocytic cell line THP-1 (TIB-202), and Vero E6 (CRL-1586) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).
D’Angelo D., Quarta E., Glieca S., Varacca G., Flammini L., Bertoni S., Brandolini M., Sambri V., Grumiro L., Gatti G., Dirani G., Taddei F., Bianchera A., Sonvico F., Bettini R, & Buttini F. (2023). An Enhanced Dissolving Cyclosporin-A Inhalable Powder Efficiently Reduces SARS-CoV-2 Infection In Vitro. Pharmaceutics, 15(3), 1023.
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