CRISPR/Cas9 genome editing of iPSCs was carried out according to our published protocol (Peters et al., 2013) . CRISPR single guide RNAs (sgRNAs) were cloned into a publically available sgRNA cloning vector and are shown in Figure S3A. Clonal cell lines were derived by limiting dilution and were screened for mutagenesis by PCR. Oligonucleotide primers used for mutagenesis screening are listed in the Key Resources Table . Double knockout of rs12740374 in these clonal cell lines was verified by Sanger sequencing (Genewiz).
APOB ELISA HLC conditioned medium was collected after twenty-four hours in culture and stored at minus eighty C until the ELISA was performed. The Human APOB ELISAPro kit (Mabtech) were used according to the manufacturer's instructions.
APOB ELISA HLC conditioned medium was collected after twenty-four hours in culture and stored at minus eighty C until the ELISA was performed. The Human APOB ELISAPro kit (Mabtech) were used according to the manufacturer's instructions.