Nah2po4 2h2o

Macronutrients used were NaNO₃ (PanReac, Barcelona, Spain) and NaH₂PO₄·2H₂O (Honeywell International Inc., Charlotte, NC, USA), while the trace elements used for media preparation were Na₂EDTA (Sigma-Aldrich, St. Louis, MO, USA), FeCl₃·6H₂O (Acros Organics, Geel, Belgium), CuSO₄·5H₂O (Sigma-Aldrich), ZnSO₄·7H₂O (Sigma-Aldrich), CoCl₂·6H₂O (Thermo Fisher Scientific, Pittsburg, PA, USA), MnCl₂·4H₂O (Acros Organics), Na₂MoO₄·2H₂O (Chem-Lab NV, Zedelgem, Belgium). Cyanocobalamin, Thiamine HCl and Biotin were purchased from Sigma-Aldrich.
Chemicals used for analysis were ammonium bicarbonate (Sigma-Aldrich), Folin–Ciocalteu reagent (Sigma-Aldrich), N,N′-dimethylformamide (DMF) (Honeywell), chloroform HPLC grade (Honeywell), mercury (III) oxide red (Sigma-Aldrich), 2,4,6-tri(2–pyridyl)–s–triazine (TPTZ) (Alfa Aesar, Ward Hill, MA, USA), water for analysis (Carlo Erba, Cornaredo, Milan, Italy), methanol HPLC grade (Thermo Fischer Scientific), sodium acetate trihydrate (Merck), acetic acid (PENTA), hydrochloric acid (HCl) (PENTA), 1,1-diphenyl-2-picrylhydrazyl radical (DPPH∙) (Sigma-Aldrich), iron (II) chloride anhydrous (Acros organics), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine) (Sigma-Aldrich), EDTA-Na₂, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (Acros organics).

Macronutrients used were NaNO₃ (PanReac, Barcelona, Spain) and NaH₂PO₄.2H₂O (Honeywell International Inc., Charlotte, NC, USA), whereas the trace elements used for media preparation were Na₂EDTA (Sigma-Aldrich, St. Louis, MO, USA), FeCl₃.6H₂O (Acros Organics, Geel, Belgium), CuSO₄.5H₂O (Sigma-Aldrich, St. Louis, MO, USA), ZnSO₄.7H₂O (Sigma-Aldrich, St. Louis, MO, USA), CoCl₂.6H₂O (Thermo Fisher Scientific, Pittsburg, PA, USA), MnCl₂.4H₂O (Acros Organics, Geel, Belgium) and Na₂MoO₄.2H₂O (Chem-Lab NV, Zedelgem, Belgium). Cyanocobalamin, Thiamine HCl and Biotin were purchased from Sigma-Aldrich, St. Louis, MO, USA.
Chemicals used for analysis were ammonium bicarbonate (Sigma-Aldrich, St. Louis, MO, USA), Folin–Ciocalteu reagent (Sigma-Aldrich, St. Louis, MO, USA), N,N′-dimethylformamide (DMF) (Honeywell International Inc., Charlotte, NC, USA), chlorophorm HPLC grade (Honeywell International Inc., Charlotte, NC, USA), mercury (III) oxide red (Sigma-Aldrich, St. Louis, MO, USA), 2,4,6-tri(2–pyridyl)–s–triazine (TPTZ) (Alfa Aesar, Ward Hill, MA, USA), water for analysis (Carlo Erba, Cornaredo, Milan, Italy), methanol HPLC grade (Thermo Fisher Scientific, Pittsburg, PA, USA), sodium acetate trihydrate (Merck, Kenilworth, NJ, USA), acetic acid (PENTA, Radiová, Prague, Czech Republic) and hydrochloric acid (HCl) (PENTA, Radiová, Prague, Czech Republic), EDTA-Na₂.

NaNO₃ (PanReac, Barcelona, Spain) and NaH₂PO₄·2H₂O (Honeywell, Tokyo, Japan) were used as nitrogen and phosphorus sources, respectively, while the trace elements Na₂EDTA (Sigma, St. Luis, MO, USA), FeCl₃·6H₂O (Acros Organics, Geel, Belgium), CuSO₄·5H₂O (Sigma), ZnSO₄·7H₂O (Sigma), CoCl₂·6H₂O (Fisher Scientific, Waltham, MA, USA), MnCl₂·4H₂O (Acros Organics), and Na₂MoO₄·2H₂O (Chem-Lab NV, Zedelgem, Belgium) were used for media preparation. Cyanocobalamin, Thiamine HCl, and Biotin were procured from Sigma-Aldrich. Chemicals used for analysis included ammonium bicarbonate (Sigma), HPLC-grade chloroform (Honeywell) and HPLC-grade methanol (Fisher Scientific).