Electrode implantation was done as described previously (Lantz et al., 2014 (link)) Electrodes were implanted in P21 – 22 mice. Mice were anesthetized with i.p. ketamine 120 mg/kg (Bioniche Pharma, Lake Forest, IL) and xylazine 9mg/kg (Akorn, Inc, Decatur, IL). Once anesthetized, 2% lidocaine jelly (Akorn, Inc, Decatur, IL) was applied locally on the scalp at the incision site. Burr holes were drilled 1.0 mm caudal from bregma and 2.0 mm lateral from the midline and ground electrodes were implanted. Tungsten microelectrodes (FHC, impedance 0.3–0.5 M ohms) were implanted in bilateral burr holes drilled at 3.00 mm lateral of midline and 0.00 mm of lambda, at a depth of 0.43 mm. Electrodes were secured with cyanoacrylate glue (Elmers, Westerville, OH). A nail glued over the rostral portion of the skull was used to secure each animal’s head during recording. After surgery, each animal was monitored until recovery of righting reflexes and was then given 0.05mg/kg of buprenorphine (Stokes Pharmacy, Mt. Laurel, NJ) for post-surgical analgesia.
Lidocaine jelly
Manufactured by Akorn
Sourced in United States
2% lidocaine jelly is a topical anesthetic formulation containing 2% lidocaine. It is used to provide local or topical anesthesia.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lidocaine jelly
1
Electrode Implantation in Mice for Electrophysiology
2
Chronic Electrodes for Visually Evoked Potentials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Visually evoked potentials are assessed in awake mice through the use of chronic implanted electrodes. For electrode implantation, P21–22 mice were anesthetized with ketamine (120 mg/kg (Bioniche Pharma, Lake Forest, IL, USA) and xylazine 9 mg/kg (Akorn, Inc., Decatur, IL, USA). Once anesthetized, 2% lidocaine jelly (Akorn, Inc., Decatur, IL, USA) was applied locally on the scalp at the incision site. Silver ground electrodes were implanted 1.0 mm caudal from bregma, and 2.0 mm lateral from the midline.
Tungsten microelectrodes (FHC, impedance 0.3–0.5 MΩ) were implanted 3.00 mm lateral of the midline and 0.5 mm of lambda, at a depth of 0.43 mm. Electrodes were secured to the skull with cyanoacrylate glue (Elmers, Westerville, OH, USA). After surgery, the animal was monitored until recovery of righting reflexes and was then given 0.05 mg/kg of buprenorphine (Stokes Pharmacy, Mt. Laurel, NJ, USA) for post-surgical analgesia.
Tungsten microelectrodes (FHC, impedance 0.3–0.5 MΩ) were implanted 3.00 mm lateral of the midline and 0.5 mm of lambda, at a depth of 0.43 mm. Electrodes were secured to the skull with cyanoacrylate glue (Elmers, Westerville, OH, USA). After surgery, the animal was monitored until recovery of righting reflexes and was then given 0.05 mg/kg of buprenorphine (Stokes Pharmacy, Mt. Laurel, NJ, USA) for post-surgical analgesia.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!