DOHH2 cells were obtained from the German Collection of Microorganisms and Cell Cultures (Leibniz-Institut DSMZ, Braunschweig, Germany). SuDHL-4 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA). Both cell lines were maintained in Roswell Park Memorial Institute medium-1640 (RPMI-1640) (Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO) and 100 units/ml penicillin and 100ug/ml streptomycin (Life Technologies, Grand Island, NY). All cell cultures were maintained at 37°C in a humidified atmosphere of 5% CO2.
Sudhl 4 cells
Manufactured by American Type Culture Collection
SuDHL-4 cells are a lymphoma cell line derived from a diffuse large B-cell lymphoma. They are useful for research purposes and can be used to study various aspects of lymphoma biology.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Gm12878 cells, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hyclone, by Cytiva (1 mentions)
Db cells, by American Type Culture Collection (1 mentions)
3 protocols using sudhl 4 cells
1
Maintenance of DOHH2 and SuDHL-4 Cell Lines
2
Culturing B-cell Lymphoma Cell Lines
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GM12878 cells, OCI-Ly7 cells, OCI-Ly10 cells and SUDHL-4 cells were obtained from American Type Culture Collection. GM12878 cells and SUDHL-4 cells were grown in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.); OCI-Ly10 cells were grown in Iscove's modified Dulbecco's medium (IMDM; Gibco; Thermo Fisher Scientific, Inc.); OCI-Ly7 cells were grown in IMDM with 0.3 g/ml glutamine. All media included 10% fetal bovine serum (HyClone; Cytiva), and all cells were cultured at 37˚C in a 5% CO2 incubator.
3
NCBP1 and METTL3 Overexpression in DLBCL
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SU-DHL-4 cells and DB cells (American Type Culture Collection, ATCC) were cultured in RPMI-1640 medium containing 10% fetal bovine serum, 100 U/mL penicillin and 100 µg/mL streptomycin, and maintained in a humidified 5% (v/v) CO2 atmosphere at a temperature of 37 °C. Cells stably expressing NCBP1 or METTL3 and control cells were subjected to viral transfection and puromycin pressure.
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