Mouse monoclonal anti eea1 antibody

Manufactured by BD

Sourced in United States

Mouse monoclonal anti-EEA1 antibody is a laboratory reagent used for the detection and localization of the Early Endosome Antigen 1 (EEA1) protein in various sample types by techniques such as immunohistochemistry and Western blotting.

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Lab products found in correlation

Mouse monoclonal anti gm130 antibody, by BD (2 mentions) Alexa fluor 568 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Hoechst 33258, by Merck Group (2 mentions) Mouse monoclonal anti neun antibody, by Merck Group (1 mentions) Doxycycline, by Fujifilm (1 mentions) D d solubilizer, by Takara Bio (1 mentions) Anti b actin mouse monoclonal antibody, by Applied Biological Materials (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Lysotracker green dnd 26, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti alpha tubulin antibody, by Merck Group (1 mentions) Rabbit polyclonal anti egfr antibody, by Cell Signaling Technology (1 mentions) Dynasore, by Merck Group (1 mentions) Anti egfr antibody, by Cell Signaling Technology (1 mentions) Streptavidin cy3, by Thermo Fisher Scientific (1 mentions) Anti lamp1 antibody, by Cell Signaling Technology (1 mentions) Rat monoclonal anti ha, by Roche (1 mentions) Mouse monoclonal anti β actin, by Merck Group (1 mentions)

5 protocols using mouse monoclonal anti eea1 antibody

Antibody Production and Characterization

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Site-specific polyclonal antibodies against human and mouse SLC18B1 were prepared by repeatedly injecting GST-fusion polypeptides encoding Met1–Thr70 of human SLC18B1 and Glu429–Thr457 of mouse SLC18B1 into rabbits, respectively. Subunit A of V-ATPase rabbit polyclonal antibody and anti-VMAT2 rabbit polyclonal antibody against synthetic peptide (Cys-Pro-Ile-Gly-Glu-Asp-Glu-Glu-Ser-Glu-Ser-Asp) prepared in-house were determined previously56 (link). The following primary antibodies were purchased from the commercial sources indicated: anti-GFAP mouse monoclonal antibody (Thermo Fisher Scientific), anti-synaptophysin mouse monoclonal antibody (Progen), anti-160-kDa Neurofilament medium mouse monoclonal antibody (Abcam), anti-PDI mouse monoclonal antibody (Abcam), anti-NeuN mouse monoclonal antibody (Millipore), anti-VAMP2 mouse monoclonal antibody (Synaptic System), anti-CgA mouse monoclonal antibody (Affinity BioReagents), anti-GM130 mouse monoclonal antibody (BD Biosciences), anti-EEA1 mouse monoclonal antibody (BD Biosciences), anti-secretogranin II mouse monoclonal antibody (Abcam), anti-cathepsin D goat polyclonal antibody (Santa Cruz Biotechnology), anti-Spm/Spd rabbit polyclonal antibody (Novus Biologicals), and anti-VAChT rabbit polyclonal antibody (Abcam).

Hiasa M., Miyaji T., Haruna Y., Takeuchi T., Harada Y., Moriyama S., Yamamoto A., Omote H, & Moriyama Y. (2014). Identification of a mammalian vesicular polyamine transporter. Scientific Reports, 4, 6836.

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Antibody Immunodetection Reagents

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The following antibodies were obtained commercially: anti-Rab11 rabbit polyclonal antibody (Invitrogen, Carlsbad, CA; #71-5300), which recognizes both Rab11A and Rab11B, anti-GM130 mouse monoclonal antibody (BD Biosciences, San Jose, CA; #610823), anti-EEA1 mouse monoclonal antibody (BD Biosciences; #610456), anti-LBPA mouse monoclonal antibody (Applied Biological Materials, Richmond, BC, Canada; #G043), anti-LAMP2 mouse monoclonal antibody (Thermo Fisher Scientific, Waltham, MA; #MA-28269), anti-TfR mouse monoclonal antibody (Invitrogen; #13-6800), anti-b-actin mouse monoclonal antibody (Applied Biological Materials; #G043), anti-ezrin mouse monoclonal antibody (Abcam, Cambridge, UK; #ab4069), horseradish peroxidase (HRP)-conjugated anti-GFP polyclonal antibody (MBL, Nagoya, Japan; #598-7), HRP-conjugated anti-mouse IgG goat polyclonal antibody (SouthernBiotech, Birmingham, AL; #1031-05), Alexa Fluor 555 + -conjugated anti-mouse IgG goat polyclonal antibody (Thermo Fisher Scientific; #A32727), and Alexa Fluor 555 + -conjugated anti-rabbit IgG goat polyclonal antibody (Thermo Fisher Scientific; #A32732). Other reagents used in this study were also obtained commercially: doxycycline (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and D/D solubilizer (Takara Bio, Shiga, Japan).

Osaki F., Matsui T., Hiragi S., Homma Y., & Fukuda M. (2021). RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11.

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Quantitative Analysis of EGF Receptor Trafficking

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Epidermal growth factor biotin conjugate (bEGF), CdSe/ZnS Qdot streptavidin conjugate emitted at 655 nm and 525 nm (savQD) were purchased from Invitrogen (USA). Native EGF and Hoechst 33258 were from Sigma-Aldrich (USA). Rabbit polyclonal anti-EGFR antibody (#2232) was from Cell Signaling Technology (USA), mouse monoclonal anti-EEA1 antibody (#610457) was from BD Transduction Lab (USA), mouse monoclonal anti-alpha-tubulin antibody (#T5168) was from Sigma (USA) and mouse monoclonal anti-Lamp-1 [H4A3] antibody (#ab25630) was from Abcam (USA). Alexa Fluor 568 goat anti-rabbit IgG, Alexa Fluor 488 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG were from Invitrogen (USA). LysoTracker Green DND-26 (#L7526) was from Invitrogen (USA). Other chemicals were from Sigma-Aldrich unless otherwise stated.

Salova A.V., Belyaeva T.N., Leontieva E.A, & Kornilova E.S. (2017). EGF receptor lysosomal degradation is delayed in the cells stimulated with EGF-Quantum dot bioconjugate but earlier key events of endocytic degradative pathway are similar to that of native EGF. Oncotarget, 8(27), 44335-44350.

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Epidermal Growth Factor Receptor Imaging

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Epidermal growth factor biotin conjugate (bEGF), CdSe/ZnS Qdot streptavidin conjugate with emission maximum at 655 nm (savQD) and Cy3-streptavidin (savCy3) were purchased from Invitrogen (USA). Mouse native EGF, dynasore and Hoechst 33258 were from Sigma-Aldrich (USA). Rabbit polyclonal anti-EGFR antibody (#2232) was from Cell Signaling Technology (USA), mouse monoclonal anti-EEA1 antibody (#610457) was from BD Transduction Lab (USA), mouse monoclonal anti-clathrin heavy chain antibody X22 (#CP45) was from Merck Millipore (Germany) and mouse monoclonal anti-HRS antibody (#ALX-804-382-C050) was from EnzoLifeSciences (USA). Alexa Fluor 568 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG were from Invitrogen (USA). Other chemicals were from Sigma-Aldrich unless otherwise stated.

Salova A.V., Belyaeva T.N., Leontieva E.A., Zlobina M.V., Kharchenko M.V, & Kornilova E.S. (2015). Quantum dots implementation as a label for analysis of early stages of EGF receptor endocytosis: a comparative study on cultured cells. Oncotarget, 7(5), 6029-6047.

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Immunoblotting Reagents for Organelle Markers

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The antisera used were rat monoclonal anti-HA (Roche; catalog #11867423001), rabbit monoclonal anti-KIAA0310 (Thermo Fisher Scientific; catalog #A300-648A-M), mouse monoclonal anti-cis-Golgi matrix protein 130 (BD Biosciences; catalog #610823), mouse monoclonal anti-CNX (Sigma-Aldrich; catalog #C7617), mouse monoclonal anti-p230 (BD Biosciences; catalog #611281), sheep polyclonal anti-TGN46 (AbD Serotec; catalog #AHP500G), mouse monoclonal anti-β-actin (Sigma-Aldrich; catalog #A5441), mouse monoclonal ANTI-FLAG M2-peroxidase (HRP; Sigma-Aldrich; catalog #A8592), mouse monoclonal anti-EEA1 antibody (BD Biosciences; catalog #610457), rabbit polyclonal anti-Rab11 antibody (gift from the De Camilli laboratory, Yale School of Medicine, New Haven, CT, self-made), rabbit monoclonal anti-Lamp1 antibody (Cell Signaling; catalog #9091S), and mouse monoclonal anti-CatD antibody (BD Biosciences; catalog #610801).
Secondary antibodies were purchased from Thermo Fisher Scientific (catalog numbers A-11034, A-11029, A-21209, A-21208, A-21203, 32260, 31470, and 32230).
Anti-Cab45 antibody was generated by the animal facility (immunization service) of the Max Planck Institute of Biochemistry as described previously (Crevenna et al., 2016 (link)). Full-length recombinant Cab45 (His-Sumo tagged) was used for rabbit immunization.

Hecht T.K., Blank B., Steger M., Lopez V., Beck G., Ramazanov B., Mann M., Tagliabracci V, & von Blume J. (2020). Fam20C regulates protein secretion by Cab45 phosphorylation. The Journal of Cell Biology, 219(6), e201910089.

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