Images for chemotaxis and dispersion assays (on gels and glass) were acquired every 5-min at 18°C using an upright microscope (DMR XA2, Leica) equipped with a motorized stage (Prior Scientific), and a camera (Orca-5G Hamamatsu). Filter wheels, camera, stage, and shutters were controlled with SimplePCI software (Hamamatsu). A 20x objective (HCX APO L 20x/0.50 W U-V-I FWD= 3.50 mm, Leica) was used.
Orca 5g
Manufactured by Hamamatsu Photonics
The Orca-5G is a high-performance scientific camera developed by Hamamatsu Photonics. It features a large active area, high quantum efficiency, and high-speed data readout. The Orca-5G is designed for a variety of scientific and industrial applications that require precise and sensitive image capture.
Automatically generated - may contain errors
2 protocols using orca 5g
1
Time-Lapse Microscopy of Chemotaxis
2
Time-Lapse Microscopy of Chemotaxis
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