EGCG was obtained from Ebeikar Tea & Extracts Co., Ltd. (Hangzhou, China). Melatonin, quercetin (Que), luteolin, chlorogenic acid, DEDTC, 2′, 7′-dichlorofluorescin diacetate (DCFH-DA), coumarin-3-carboxylic acid (3-CCA), bathocuproinedisulfonic acid disodium salt (BCS), neocuproine, catalase, mannitol, superoxide dismutase (SOD), histidine, and methionine were purchased from Sigma (St. Louis, Missouri, USA). Plasmid pBR322 DNA was obtained from New England Biolabs (Beijing, China). ECL Plus reagent and Polyvinylidene difluoride (PVDF) membrane were products of Bio-Rad Laboratories, Inc. (Hercules, CA, USA). The primary antibodies against γ-H2AX (sc-101696) and anti-mouse (sc-2489) secondary antibody were obtained from Santa Cruz (Dallas, TX, USA). The primary antibodies against β-actin (A5441) were purchased from Sigma. The primary antibodies against cleaved caspase 3 (9662), cleaved caspase 8 (8592), PARP (9542) and anti-rabbit (7074) secondary antibodies were products of Cell Signal Technology, Inc. (Danvers, MA, USA). The primary antibody against thrombospondin-1 (TSP-1) (ab85762) and ceruloplasmin (ab48614) were products of Abcam (Cambridge, UK). Other chemicals were of the highest grade available.
Dedtc
Manufactured by Merck Group
Sourced in United States
DEDTC is a chemical compound used in laboratory applications. It is a chelating agent that forms stable complexes with various metal ions. The core function of DEDTC is to facilitate the analysis and separation of these metal ions in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Chlorogenic acid, by Merck Group (1 mentions)
Ecl plus reagent, by Bio-Rad (1 mentions)
Luteolin, by Merck Group (1 mentions)
Catalase, by Merck Group (1 mentions)
Mannitol, by Merck Group (1 mentions)
Melatonin, by Merck Group (1 mentions)
Primary antibodies against β actin a5441, by Merck Group (1 mentions)
Methionine, by Merck Group (1 mentions)
Ab48614, by Abcam (1 mentions)
Ab85762, by Abcam (1 mentions)
Polyvinylidene difluoride pvdf membrane, by Bio-Rad (1 mentions)
Quercetin que, by Merck Group (1 mentions)
Lsm510 meta confocal laser microscope, by Zeiss (1 mentions)
Znaf 2 da, by Enzo Life Sciences (1 mentions)
2 protocols using dedtc
1
Antioxidant Activity of EGCG and Melatonin
2
Zn2+ Imaging in Taste Bud Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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SD rats (3 rats per group) were perfused with saline under deep isoflurane anesthesia, and epithelial tissues, including taste buds, were removed from the tongue by treatment with 1.0 mg/mL collagenase D, 2.5 mg/mL dispase II, and 1.0 mg/mL trypsin inhibitor. As negative controls, rats received 0.7 g/kg diethyldithiocarbamate (DEDTC; Sigma-Aldrich) intraperitoneally 80 min before perfusion with saline. DEDTC was used as a chelating agent for Zn2+ and other group IIB metal ions.44 (link) Subsequently, the detached epithelial tissues were treated with Tyrode’s solution (140 mM NaCl, 5 mM KCl, 1 mM CaCl2 (link), 1 mM MgCl2 (link), 10 mM D-glucose, 10 mM HEPES, and 10 mM sodium pyruvate) containing 100 mM ZnAF-2 DA (Enzo Life Sciences, Farmingdale, NY, USA) and 0.04% Pluronic F-127 for 90 min at 23°C, followed by washing in Tyrode’s solution. Fluorescence images were taken using an LSM510 META confocal laser microscope (Carl Zeiss, Jena, Germany).
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