Rabbit anti par2

The expression of protease-activated receptor 2 (PAR-2), claudin-1, and claudin-2 was studied by using immunofluorescence. The histologic sections of the stomach, jejunum, ileum, and colon segments at 3 hours or 6 hours after the operation were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned in 4 μm thick sections. After deparaffinization, rehydration, and rinsing with standard methods, the slide sections were incubated with the primary antibody rabbit anti-PAR2 (1:500; Santa Cruz Biotechnology) overnight at 4°C, and then incubated with goat anti-rabbit IgG-fluorescein isothiocyanate (FITC) (1:200; Santa Cruz Biotechnology) for 1 hour at 37°C. Similarly, tight junction (TJ) proteins were measured with immunofluorescence staining by using the procedure described above. Tissues were incubated with the primary antibody for claudin-1 (1:50; Invitrogen, South San Francisco, CA, USA) or claudin-2 (1:200; Invitrogen) overnight at 4°C, then washed and incubated with the secondary antibody goat anti-rabbit IgG-FITC (1:200; Santa Cruz Biotechnology) for 30 minutes at 37°C. The stained images were examined under a fluorescence microscope (Zeiss Axio Imager Z1; Carl Zeiss). Images analysis was performed by using MetaMorph (MDS Analytical Technologies, Sunnyvale, CA, USA).