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2 protocols using cd28 fitc

1

Analyzing Cell Surface Signaling

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Anti-SH3P × 1 (SNX9) (1:100, Abcam Australia Pty Ltd, Cat# 181856), CD28-FITC (1:100, eBioscience, Cat# 11028942), Alexa Fluor 647 anti-CD247 (pY142) (1:20, BD Phosflow, Cat# 558489), Anti-phospho-CD28 (pTyr218) (1:20, Merck Cat# SAB4504133), anti-Rabbit Alexa647 (Thermo Fisher Scientific Cat# A-21245).
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2

Multiparameter Flow Cytometry Immunophenotyping

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The following conjugated Anti-human monoclonal antibodies (mAbs) were purchased from BD Pharmingen, USA: CD3 PE-Cy7, CD4 FITC, CD4 PerCP-Cy5.5, CD4 APC, CD8 PerCP-Cy5.5, CD14 PErCP-Cy5.5, CD14 PE, CD80 FITC, CD86 PE, CD152 PE, HLA-DR APC, Ki67 PE, Annexin V FITC, and Propidium Iodide. CD28 FITC and CD45 Pacific blue were purchased from eBioscience, USA.
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