Concentrations of mouse BUN (MyBioSource, Inc., MBS751125), Creatinine (Cr, MyBioSource, Inc., MBS2540563), ASpartate Transaminase (AST, MyBioSource, Inc., MBS8800779), ALanine Transaminase (ALT, MyBioSource, Inc., MBS2022063), LDH (MyBioSource, Inc., MBS8801243), CK-MB ELISA kit (BioVision, Milpitas, E4607), Troponin I (MyBioSource, Inc., MBS5316602), Caspase-3 (MyBioSource, Inc., MBS733100), ATP (MyBioSource, Inc., MBS724442), Caspase-9 (LifeSpan BioSciences, LS-F32788), GSH (MyBioSource, Inc., MBS267424), GPX (MyBioSource, Inc., MBS776262), SOD (MyBioSource, Inc., MBS034842), Mitochondrial complex respiration I (complex I, MyBioSource, Inc., MBS912812), Mitochondrial complex respiration II (complex II, MyBioSource, Inc., MBS108909), Mitochondrial complex respiration III (complex III, Abcam, ab287844), Lactic Acid (Creative Diagnostics, DEIA-BJ2418), Bax (Abcam, ab233624) were measured using ELISA kits according to the manufacturer’s instructions.
Caspase 3
Manufactured by MyBioSource
Sourced in United States
Caspase-3 is a protein that plays a crucial role in the process of apoptosis, or programmed cell death. It is an essential enzyme involved in the cleavage of various cellular substrates, leading to the characteristic morphological and biochemical changes associated with apoptosis. Caspase-3 is considered a key executioner caspase, as it is responsible for the proteolytic cleavage of many key cellular proteins, ultimately resulting in cell death.
Automatically generated - may contain errors
Lab products found in correlation
Glutathione (gsh), by MyBioSource (1 mentions)
Ck mb elisa kit, by Abcam (1 mentions)
Adenosine triphosphate (atp), by MyBioSource (1 mentions)
Troponin 1, by MyBioSource (1 mentions)
Kim 1, by Abcam (1 mentions)
Cystatin c, by Abcam (1 mentions)
Tnf α, by Sangon (1 mentions)
Hmgb1, by Sangon (1 mentions)
Neuron specific enolase, by R&D Systems (1 mentions)
Bcl 2, by MyBioSource (1 mentions)
Bcl2 associated x (bax), by MyBioSource (1 mentions)
Kits for the quantification of, by MyBioSource (1 mentions)
Ck mb, by MyBioSource (1 mentions)
Rat enzyme linked immunosorbent assay, by MyBioSource (1 mentions)
5 protocols using caspase 3
1
Comprehensive Cardiac and Metabolic Profiling
2
Quantifying Kidney Injury Biomarkers
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To determine the level of renal tissue injury, we quantified the total protein levels of NGAL, KIM‐1, cystatin C, and casapse‐3 by ELISA in whole kidneys obtained from all experimental groups 7 days post‐I/R injury. Each marker level was measured in duplicates by sandwich ELISA techniques using commercially available rat NGAL (Enzo life science), KIM‐1, cystatin C (Abcam), and caspase‐3 (Mybiosource, San Diego, CA) immunoassay kits according to the manufacturer's instructions.
3
Biomarkers in Epilepsy Seizure Pathogenesis
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Around 5 mL of fasting blood was collected from each TLE patient within 12 h after an epileptic seizure and from each control. After centrifugation of peripheral blood at a speed of 3000 r/min for 15 min, supernatants were gathered and stored at −80℃. Then, the levels of interleukin-2 (IL-2) (Sangon, Shanghai, China), tumor necrosis factor-α (TNF-α) (Sangon), Interferon-γ (IFN-γ) (eBioscience, San Diego, CA, USA), high mobility group box protein 1 (HMGB-1) (Sangon), S100B (Boster, Wuhan, China), neuron specific enolase (NSE) (R&D systems, Minneapolis, MN, USA), glial fibrillary acidic protein (GFAP) (Boster, Wuhan, China), calcitonin gene related peptide (CGRP) (R&D systems), Bcl-2 (MyBioSource, San Diego, CA, USA), Bax (MyBioSource), and Caspase-3 (MyBioSource) were determined using respective ELISA kits.
4
Cardioprotective Effects of CCK-8 in Rats
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The experiments were approved by the Central Hospital Affiliated to Shandong First Medical University, under the number: 2020-0618-01. All experiments were performed as per institutional ethical guidelines in the Research Center of the Central Hospital Affiliated to Shandong First Medical University, Jinan, China.
In this investigation, Wistar albino rats of weight 210-240g were used. The doses of CCK-814 , Proglumide15 , heaxmethonium16 were selected as per literature reports. The kits for the quantification of lactate dehydrogenase 1 (LDH-1), MB isoform of creatine kinase (CK-MB), cardiac troponins (cTnT), Bcl-2, caspase 3 and CCK were procured from MyBioSource, Inc. San Diego, CA USA.
In this investigation, Wistar albino rats of weight 210-240g were used. The doses of CCK-8
5
Rotenone-Induced Neuroinflammation and Apoptosis
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Sigma-Aldrich,
USA, provided the rotenone and other chemicals acquired from authenticate
sources were of analytical quality. Hirsutidin was received as a gifted
from SRL, India. Rat enzyme-linked immunosorbent assay (ELISA) kit
analysis of tumor necrosis factor-α (TNF-α), interleukins-1β
(IL-1β), IL-6, and caspase-3 (MyBioSource, USA) were used to
quantity.
USA, provided the rotenone and other chemicals acquired from authenticate
sources were of analytical quality. Hirsutidin was received as a gifted
from SRL, India. Rat enzyme-linked immunosorbent assay (ELISA) kit
analysis of tumor necrosis factor-α (TNF-α), interleukins-1β
(IL-1β), IL-6, and caspase-3 (MyBioSource, USA) were used to
quantity.
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