White 96 well clear bottomed plates

Manufactured by Corning

The Corning 96-well clear-bottomed plates are a laboratory equipment product designed for various experimental applications. These plates feature a clear bottom to facilitate optical measurements and observations.

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Lab products found in correlation

Infinite 2000 pro reader, by Tecan (3 mentions) Celltiter glo kit, by Promega (3 mentions) Crystal violet, by Merck Group (1 mentions) A scanner, by Epson (1 mentions) Clariostar spectrophotometer, by BMG Labtech (1 mentions) Crystal violet, by BMG Labtech (1 mentions) Celltiter glo, by Corning (1 mentions)

3 protocols using white 96 well clear bottomed plates

Quantitative Melanoma Cell Growth Assay

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For the purpose of monitoring growth rate, 5×10³ melanoma cells were seeded in white 96-well clear-bottomed plates (Corning) and growth was monitored every 24h using CellTiter Glo kit (Promega) according to the manufacturer’s recommendations. Luminescence intensity was measured using an Infinite 2000 Pro reader (Tecan). Alternatively, 5 × 10⁵ cells transfected with siRNA were seeded per well, in 6-well plates. After 24h, cells were fixed and stained using a ready-to-use solution of crystal violet (Merck). Plates were pictured using a scanner (Epson). Growth was quantified by solubilization of crystal violet in 10% acetic acid and monitoring optical density at 595nm using a Clariostar spectrophotometer (BMG Labtech).

Lazar I., Fabre B., Feng Y., Khateb A., Turko P., Martinez-Gomez M.J., Frederick D.T., Levesque M.P., Feld L., Zhang G., Zhang T., James B., Shklover J., Avitan Hersh E., Livneh I., Scortegagna M., Brown K., Larsson O., Topisirovic I., Wolfenson H., Herlyn M., Flaherty K., Dummer R, & Ronai Z.A. (2020). SPANX control of Lamin A/C modulates nuclear architecture and promotes melanoma growth. Molecular cancer research : MCR, 18(10), 1560-1573.

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Cell Viability and Growth Assay

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Cell viability and growth were assayed using the CellTiter Glo kit (Promega) according to the manufacturer’s recommendations. Cell lines were plated in white 96-well clear-bottomed plates (Corning) at a density of 7 × 10³ cells/well, and growth was monitored every 24 h using CellTiter Glo reagent. Viability was quantified by measuring luminescence intensity with an Infinite 2000 Pro reader (Tecan).

Khateb A., Deshpande A., Feng Y., Finlay D., Lee J.S., Lazar I., Fabre B., Li Y., Fujita Y., Zhang T., Yin J., Pass I., Livneh I., Jeremias I., Burian C., Mason J.R., Almog R., Horesh N., Ofran Y., Brown K., Vuori K., Jackson M., Ruppin E., Deshpande A.J, & Ronai Z.A. (2021). The ubiquitin ligase RNF5 determines acute myeloid leukemia growth and susceptibility to histone deacetylase inhibitors. Nature Communications, 12, 5397.

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Cell Viability Assay Using CellTiter Glo

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Cell viability and growth were assayed using CellTiter Glo kit (Promega) according to the manufacturer's recommendations. Cell lines were plated in white 96-well clear-bottomed plates (Corning) at a density of 7 × 10 3 cells/well and growth was monitored every 24 h using CellTiter Glo reagent. Viability was quantified by measuring luminescence intensity with an Infinite 2000
Pro reader (Tecan).

Khateb A., Deshpande A., Feng Y., Lee J.S., Lazar I., Fabre B., Li Y., Finlay D., Fujita Y., Zhang T., Yin J., Pass I., Livneh I., Burian C., Mason J.R., Almog R., Horesh N., Ofran Y., Brown K.M., Vuori K., Jackson M.R., Ruppin E., Deshpande A.J., & Ronai Z.A. (2020). RNF5 Regulation of RBBP4 Defines Acute Myeloid Leukemia Growth and Susceptibility to Histone Deacetylase Inhibitors.

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