Anti sqstm1 p62 8025

Manufactured by Cell Signaling Technology

Anti-SQSTM1/p62 (8025) is a product offered by Cell Signaling Technology. It is a laboratory equipment used for research purposes. The product's core function is to detect the SQSTM1/p62 protein, which plays a role in various cellular processes.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Anti lc3b, by Abcam (1 mentions) Alexa fluor 488 and 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Anti icp5, by Abcam (1 mentions) Prolong diamond antifade mounting media, by Thermo Fisher Scientific (1 mentions) Axio observer, by Zeiss (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti lamin b1 12987 1 ap, by Proteintech (1 mentions) Lysotrakerred, by Thermo Fisher Scientific (1 mentions) Anti p53 10442 1 ap, by Proteintech (1 mentions) Anti gpx4 sc 166570, by Santa Cruz Biotechnology (1 mentions) Mitosox red, by MedChemExpress (1 mentions)

2 protocols using anti sqstm1 p62 8025

Immunofluorescence Analysis of Autophagy Markers

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Cells were grown at 50% confluency on 13 mm diameter coverslips and were fixed with 100% methanol on ice, before washing them with 1X phosphate buffered saline (PBS). Blocking was done in 10%-FBS. PBS and cells were permeabilized with 0.1% triton. Staining was done in a moist chamber. Images were taken with 63X oil immersion lens on widefield fluorescence microscope (Zeiss Axio Observer). Antibodies used for immunofluorescence were anti-LC3B (1:500 dilution, Abcam), anti-SQSTM1/p62 (8025, Cell Signaling 1:500), anti-ICP5 (1:500 dilution, Abcam), Alexa Fluor-488 and -594 conjugated secondary antibodies (1:750 and 1:1000 dilutions respectively, Life Technologies). Coverslips were mounted to the slides using DAPI-containing ProLong Diamond antifade mounting media (Thermo Fisher, Waltham, MA). Lysotracker staining was performed following the instructions of the manufacturer (ThermoFisher, Waltham, MA).

Hait A.S., Olagnier D., Sancho-Shimizu V., Skipper K.A., Helleberg M., Larsen S.M., Bodda C., Moldovan L.I., Ren F., Brinck-Andersen N.S., Thomsen M.M., Freytag M.R., Darmalinggam S., Parkes I., Kadekar D.D., Rahbek S.H., van der Horst D., Kristensen L.S., Eriksson K., Kjems J., Mostowy S., Christiansen M., Mikkelsen J.G., Brandt C.T., Paludan S.R, & Mogensen T.H. (2020). Mutations in LC3B2 and ATG4A underlie recurrent HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans. Science immunology, 5(54), eabc2691.

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Ferroptosis-related Molecular Mechanisms

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Erastin (HY-15763), ferrosatatin-1 (HY-100579), N-acetylcystein (HY–B0215) and MitoSOX Red (HY-D1055) were purchased from MedChem Express (Monmouth Junction, NJ). All-trans retinoic acid (ATRA, R2625), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, M2003) and 4′,6-diamidino-2-phenylindole (DAPI, D9542) were from Merck KGaA (Darmstadt, Germany). Anti-CYTL1 (ab129767) antibody was purchased from Abcam (Cambridge, UK). Anti-CSE (12217-1-AP), anti-ATG5 (10181-2-AP), anti-Keap1 (10503-2-AP), anti-Lamin B1 (12987-1-AP) and anti-p53 (10442-1-AP) antibodies were from Proteintech (Chicago, IL). Anti-HA (3724), anti-LC3B (3868), anti-Myc (2276) and anti-SQSTM1/p62 (8025) antibodies were from Cell Signaling Technology (Beverly, MA). Anti-GAPDH (sc-47724), anti-β-tubulin (sc-5274), anti-β-actin (sc-47778), anti-CBS (sc-133154), anti-Nrf2 (sc-365949), and anti-GPX4 (sc-166570) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). Lipofectamine 3000 (L3000), Lipofectamine RNAi MAX (13778), the lysosome-specific dye LysoTraker Red (L7528), and Alexa Fluor 488 goat anti-rabbit IgG (R37116) were purchased from Thermo Fisher Scientific (Waltham, MA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

Xue W., Yu Y., Yao Y., Zhou L., Huang Y., Wang Y., Chen Z., Wang L., Li X., Wang X., Du R., Shen Y, & Xu Q. (2024). Breast cancer cells have an increased ferroptosis risk induced by system xc− blockade after deliberately downregulating CYTL1 to mediate malignancy. Redox Biology, 70, 103034.

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