Cebpb

Manufactured by Proteintech

Sourced in United States

CEBPB is a DNA-binding protein that functions as a transcriptional activator. It binds to regulatory regions of genes and enhances their expression. CEBPB is involved in the regulation of genes related to cellular differentiation, immune response, and inflammation.

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Lab products found in correlation

Interleukin 6 (il 6), by Proteintech (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Gapdh, by Proteintech (1 mentions) Ripa lysis buffer, by Keygene (1 mentions) Cd163, by Abcam (1 mentions) P jak1, by Proteintech (1 mentions) Pg112, by Ipsen (1 mentions) Ipvh00010, by Merck Group (1 mentions) Stat3, by Proteintech (1 mentions) P stat3, by Proteintech (1 mentions) β actin, by Proteintech (1 mentions)

2 protocols using cebpb

Western Blot Analysis of Inflammatory Markers

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Total protein was extracted using the RIPA lysis buffer (1:1000) (KeyGene Biotech, Nanjing, China), and the supernatant was collected. Protein quantification was performed using the bicinchoninic acid assay (KeyGene Biotech). The extracted total protein was separated into a 10% sodium dodecyl sulfate-polyacrylamide (SDS) gel electrophoresis and transferred onto a polyvinylidene difluoride membrane (Merck Millipore, Burlington, MA, USA). The membrane was then blocked for 1 hour in Tris-buffered saline with Tween 20 containing 5% non-fat milk. Next, we incubated the membrane with the following primary antibodies overnight at 4°C: YY1 (1/2000, 68 kDa; Abcam), CD163 (1/2000, 150 kDa; Abcam), P300 (1/2000, 300 kDa; Abcam), IL-6 (1/2000, 20 kDa; Proteintech, Rosemont, IL, USA), P65 (1/2000, 65 kDa; Proteintech), and CEBPB (1/3000, 45 kDa; Proteintech). GAPDH (1/3000, 37 kDa; Proteintech) was used as an internal control.

Chen S., Lu K., Hou Y., You Z., Shu C., Wei X., Wu T., Shi N., Zhang G., Wu J., Chen S., Zhang L., Li W., Zhang D., Ju S., Chen M, & Xu B. (2023). YY1 complex in M2 macrophage promotes prostate cancer progression by upregulating IL-6. Journal for Immunotherapy of Cancer, 11(4), e006020.

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Comprehensive Protein Analysis via Western Blot

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Lysate (Beyotime P0013J) containing protease inhibitor and EDTA was utilized to lyse tissues and cells. Protein was then separated by 10% PAGE gels (Epizyme PG112) and transferred onto PVDF membranes (Millipore IPVH00010). The PVDF membranes were blocked with 10% bovine serum albumin (BSA) and incubated with primary antibodies β-actin (CST 3700S), A1AT (Proteintech 16382-1-AP), c-myc (SANTA sc-40), lamin B1 (CST13435S), p-JAK1 (CST 3331S), JAK1 (CST 3332S), p-STAT3 (CST 9145S), STAT3 (CST 4904S) and CEBPB (Proteintech 23431-1-AP) at 4 °C overnight. Then, the membranes were incubated with secondary antibody (Zsbio zb-2301 and Zsbio zb-2305) at room temperature for 1 h.

Ma Y., Chen Y., Zhan L., Dong Q., Wang Y., Li X., He L, & Zhang J. (2024). CEBPB-mediated upregulation of SERPINA1 promotes colorectal cancer progression by enhancing STAT3 signaling. Cell Death Discovery, 10, 219.

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