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2 protocols using nitrosodiethylamine

1

Evaluation of Nanoassemblies for CML Detection

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Sprague-Dawley rats, Swiss albino mice, newly synthesized nanoassemblies (PFT-HSA-TFT-Cy7 and PFT-Hcy-HSA-Cy7), CML detection ELISA kit (bioassay technology laboratory), TRIzol Reagent (Ambion), primers (Eurofins), Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific), real-time PCR (Himedia). Hi-SYBr Master Mix, Agarose, Tris buffer, ethidium bromide, bromophenol blue, Protein Estimation Kit (G Biosciences), DNPH, methylglyoxal, bovine serum albumin, PBS, antibiotic antimycotic solution, and trypsin (all these chemicals were purchased from Himedia), glucometer (One Touch Select simple), 0.2 μm syringe filter (Sartorius), DCFDA dye, nitrosodiethylamine, alloxan monohydrate (all these chemicals were purchased from Sigma Aldrich), UV-spectrophotometer (Eppendorf), spectrofluorometer (Agilent technologies, Carry Eclipse), phenobarbitone (Abbot), RPMI (Cell Clone), fetal bovine serum (Gibco), and RNA later (Invitrogen).
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2

Liver Tissue Characterization Protocol

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Chemicals. Acetaminophen (AP), α-naphthylisothiocyanate (ANIT), chloramphenicol (CH), chlorpromazine (CP), colchicine (CL), cyclosporine (CS), ethinyl estradiol (EE), methyl testosterone (MT), and nitroso diethylamine (ND) were purchased from Sigma-Aldrich (St.Louis, MO, USA). Benziodarone (BD) was purchased from Kemprotec Limited (Middlesbrough, United Kingdom). Stock solutions were prepared for all the compounds in DMSO (VWR, Briare, France) except for APAP. For APAP, stock solutions were prepared using the WME medium. RNAlater reagent was purchased from Sigma-Aldrich (St.Louis, MO, USA).
Human Liver Tissue. Human tissue was obtained from the remaining liver tissue after split liver transplantation (TX) from six donors. The characteristics of the human livers used in the experiments are described in Table 1. The experimental protocols were approved by the Medical Ethical Committee of the University Medical Center Groningen.
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