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8 protocols using ammonium formiate

1

Analytical Standards for Ciguatoxin Detection

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C-CTX1 pure standard solution (5 ng·mL−1) was kindly provided by Dr. Robert Dickey (previously, U.S. Food and Drug Administration) via Dr. Ronald Manger (Fred Hutchinson Cancer Research Center, Seattle, USA). A qualitative mixture of P-CTXs standard solution containing: CTX1B, 2,3-dihydroxyCTX3C, 51-hydroxyCTX3C, 52-epi-54-deoxyCTX1B/54-deoxyCTX1B, 2-hydroxyCTX3C, 49-epiCTX3C, CTX3C, CTX4A and CTX4B, was kindly provided by Prof. Takeshi Yasumoto (Japan Food Research Laboratories, Japan)
Acetone, diethyl ether, methanol, water, hexane and ethyl acetate used for extraction and purification were of HPLC grade (Merck KGaA, Darmstadt, Germany). Methanol, Acetonitrile, formic acid, ammonium formiate (Merck KGaA, Darmstadt, Germany) and water (J. T. Baker, Center Valley, PA, USA) for LC-MS analysis were of LC-MS grade. Methanol, formic acid, ammonium formiate (Merck KGaA, Darmstadt, Germany) for HPLC fractionation were of HPLC grade. Water for fractionation was deionized and purified at 15 MΩ·cm−1 through a Milli-Q Gradient A10 system (Millipore, Molsheim, France)
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2

Ciguatoxins Extraction and Purification

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Acetone, diethyl ether, methanol, water, hexane and ethyl acetate used for ciguatoxins extraction and purification were of HPLC grade (Merck KGaA, Darmstadt, Germany). Methanol, formic acid, ammonium formiate (Merck KGaA, Darmstadt, Germany) and water (J. T. Baker, Center Valley, PA, USA) were of LC–MS grade.
Pure standard solution of CTX1B, a mixture of Pacific Ciguatoxins standard solution containing CTX1B, 2,3-dihydroxi-CTX3C, 51-hydroxi-CTX3C, 52-epi-54-deoxy-CTX1B/54-deoxy-CTX1B, 49-epi-CTX3C/CTX3C, CTX4A/CTX4B were kindly supplied by Prof. Takeshi Yasumoto (Japan Food Research Laboratories). C-CTX1 pure standard solution was kindly provided by Dr. Robert Dickey (previously, U.S. Food and Drug Administration) via Dr. Ronald Manger (Fred Hutchinson Cancer Research Center, Seattle, USA).
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3

Quantification of Duloxetine Hydrochloride

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The reference standard duloxetine hydrochloride and the internal standard D3‐duloxetine oxalate were purchased from AlsaChim, Illkirch Graffenstaden, France. Ammonium acetate, ammonium formiate, acetic acid, dimethylsulfoxide, methanol, n‐hexane, tert‐butyl‐methyl ether, and water for chromatography were of analytical or HPLC grade, purchased from either Merck (Darmstadt, Germany) or Fluka (Leipzig, Germany).
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4

Lipidomic Analysis of Oxidized Phospholipids

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Hexane, hexadecane, Na2SO4, MES, TRIS, EGTA, ammonium formiate, 5-doxyl stearic acid, DOPC, DOPE, cardiolipin and arachidonic acid were purchased from Sigma Aldrich GmbH (Germany). ONE, HNE and HHE came from Cayman Chemicals. E. coli polar lipid and DPhPC, were purchased from Avanti polar lipids. ULC/MS grade methanol was supplied by Biosolve BV (Valkenswaard, Netherlands). Chloroform was from Merck KGaA (Darmstadt, Germany).
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5

Analytical Grade Chemical Characterization

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All chemicals and reagents used were of analytical grade. Pure water was obtained from a Milli-Q system (Millipore, Darmstadt, Germany). Acyclovir, ganciclovir, bromouracil, methanol, acetonitrile, trifluoracetic acid, ammonium formiate, and formic acid were purchased by Sigma-Aldrich (Milan, Italy). Phree Phospholipid Removal Products were purchased from Phenomenex (Bologna, Italy).
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6

Quantification of Polyphenols by HPLC

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Formic acid, HPLC grade methanol, acetone, hydrochloric acid, trifluoroacetic acid, ammonium formiate, L-ascorbic acid, and phloroglucinol were purchased from Sigma Aldrich (St Louis, MO, USA). Deionized water was obtained from a Milli-Q purification system (Millipore, Molsheim, France). Standards of (+)-catechin, (–)-epicatechin, (–)-epicatechin 3-O-gallate, reduced L-glutathione, oxidized L-glutathione, piceatannol, p-coumaric acid, protocatechuic acid, syringic acid, trans-caftaric acid, and trans-resveratrol were purchased from Sigma-Aldrich (St Louis, MO, USA). Standards of (–)-epigallocatechin, gallic acid, hydroxytyrosol, malvidin 3-O-glucoside chloride, malvidin 3,5-di-O-glucoside chloride, procyanidin B2, quercetin 3-O-glucuronide, and taxifolin were purchased from Extrasynthese (Geney, France). Standards of caffeic acid, ferulic acid, and vanillic acid were purchased from Fluka (Buchs, Switzerland). Standards of 1-O-Galloyl-β-D-glucose and quercetin 3-O-glucoside were purchased from PlantMetaChem, Transmit GmbH (Gießen, Germany). Standard of trans-piceid was purchased from Selleckchem (Houston, TX, USA).
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7

Analysis of Polyphenolic Compounds

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Formic acid and HPLC grade methanol were purchased from VWR Prolabo (Fontenay-sous-Bois, France). Deionized water was obtained from a Milli-Q purification system (Millipore, Molsheim, France). Standard of trans-piceid was purchased from Selleckchem (Houston, TX, USA). Standards of caffeic acid, vanillic acid, gallic acid and ferulic acid were purchased from Fluka (Buchs, Switzerland). Standards of syringic acid, p-coumaric acid, protocatechuic acid, ethyl protocatechuate, tyrosol, tryptophol, tryptophan, trans-resveratrol, quercetin, catechin, epicatechin, epicatechin 3-gallate, taxifolin, oxidized and reduced glutathione were purchased from Sigma-Aldrich (St. Louis, MO, USA), as well as hydrochloric acid, phloroglucinol, L-ascorbic acid, and ammonium formiate. Standards of ethyl gallate, ethyl caffeate, tyrosine, hydroxytyrosol, epigallocatechin, malvidin 3-O-glucoside chloride, and malvidin 3,5-diglucoside chloride were purchased from Extrasynthese (Geney, France). Standards of trans-caftaric acid, quercetin glucoside and procyanidin dimers B1, B2, B3, B4, and trimer C1 were purchased from Phytolab (Vestenbergsgreuth, Germany).
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8

Analytical Standards for Phenolic Compounds

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HPLC grade formic acid, methanol and N,N-dimethyformamide were purchased from VWR Prolabo (Fontenay-sous-Bois, France). Acetaldehyde, acetic acid, acetone, hydrochloric acid, lithium chloride, phloroglucinol, L-ascorbic acid, sodium hydrogen sulfite and ammonium formiate were purchased from Sigma Aldrich. Deionized water was obtained from a Milli-Q purification system (Millipore, Molsheim, France).
Standards of caffeic acid (>99%), vanillic acid (>99%) and ferulic acid (>99%) were purchased from Fluka (Buchs, Switzerland). Standards of syringic acid (>99%), p-coumaric acid (>98%), quercetin (>95%), (+)catechin (>99%), (-)-epicatechin (>98%), (-)-epicatechin 3-O-gallate (>97.5%), protocatechuic acid (>95%), reduced and oxidized glutathione (>98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Standards of malvidin 3-O-glucoside chloride (>95%) and malvidin 3,5-diglucoside chloride (>97%) were purchased from Extrasynthese (Geney, France). Standards of trans-caftaric acid (>94%), quercetin 3-O-glucoside (>90%) and procyanidin dimer B2 (>90%) were purchased from Phytolab (Vestenbergsgreuth, Germany).
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