Mouse anti his

TSP or purified proteins were separated using 10–12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under the reducing condition, and the proteins on the gels were either stained with 0.25% Coomassie blue R-250 (AMRESCO, cat. no: 6104-59-2, Zottegem, Belgium) solution or analyzed by Western blotting using mouse anti-His (Qiagen, Valenica, CA, USA), mouse anti-LIF (abcam, cat. no., ab34427, Cambridge, UK), mouse anti-GFP (Clontech, cat. No., 632381, CA, USA), rat anti-HA (Roche, cat. No., 1583 816, Basel, Switzerland), mouse anti-IL6 (abcam, cat. no., ab9324, Cambridge, UK), and anti-CBD (Bioapp, Pohang, Korea) antibodies. The horseradish peroxidase (HRP)-conjugated sheep anti-mouse IgG (Bethyl, cat. no., A90-146P, TX, USA), and goat anti-rat IgG (Bethyl, cat. no., A110-105P, TX, USA) were used as secondary antibody. Bands on the immunoblots were detected using chemiluminescence detection reagents (GE healthcare, Illinois, USA), and images were captured with a LAS 4000 image capture system (Fujifilm, Tokyo, Japan).

Mouse anti-Plk4 antibody has been described previously (Cizmecioglu et al., 2010 (link)) and used at a final concentration of 1 µg/ml. Mouse anti-Flag M2 (F3165), mouse anti-α-tubulin (T5168) and mouse anti-γ-tubulin (T6557) were from Sigma. Mouse anti-Myc (9E10), mouse anti-Plk1 (F-8), and mouse anti-cyclin E (HE12) were obtained from Santa Cruz Biotechnology. Mouse anti-actin (JLA20) was from Calbiochem, mouse anti-His from Qiagen, rabbit anti-GFP (NB600-303) from Novus, rabbit anti-CP110 (A301-343A) and rabbit anti-STIL (A302-442A) for western blotting from Bethyl, rabbit anti-STIL (ab89314) for immunofluorescence and rabbit anti-pericentrin (ab4448) from Abcam. Rabbit anti-cyclin B1 has been described previously (Hoffmann et al., 1993 (link)). Secondary antibodies for western blotting were peroxidase-conjugated donkey anti-rabbit (Jackson Laboratories) and goat anti-mouse (Novus). Secondary antibodies for immunofluorescence were goat anti-mouse IgG and goat anti-rabbit IgG coupled to Alexa Fluor 488 or Alexa Fluor 594 (Molecular Probes).