Rabbit anti neurod1 antibody

Manufactured by Abcam

Sourced in United States

Rabbit anti-NeuroD1 antibody is a primary antibody that specifically recognizes the NeuroD1 protein. NeuroD1 is a transcription factor that plays a critical role in the development and differentiation of neuronal cells. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to detect and study the expression of NeuroD1 in biological samples.

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Lab products found in correlation

Horseradish peroxidase hrp conjugated secondary antibody, by Proteintech (1 mentions) Mouse anti acetylated tubulin monoclonal antibody, by Proteintech (1 mentions) Anti gapdh antibody, by Abcam (1 mentions) Actin, by Abcam (1 mentions) Hrp conjugated anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti ascl1 antibody, by BD (1 mentions) Mini protean tgx stain free protein gel, by Bio-Rad (1 mentions) Mouse anti kpnb1 antibody, by Santa Cruz Biotechnology (1 mentions) Pierce protein g agarose beads, by Thermo Fisher Scientific (1 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (1 mentions)

2 protocols using rabbit anti neurod1 antibody

Protein Extraction and Western Blot Analysis

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For protein extraction, RIPA buffer was used to lyse cells and tissue (10 mm of the spinal cord containing the injury epicenter). Protein concentrations were detected by using a Bicinchoninic acid (BCA) assay kit. The denatured proteins were electrophoretically transferred to a 10% SDS-PAGE gel and subsequently transferred to polyvinylidene fluoride (PVDF) membranes. The membranes were incubated at 4°C overnight with a mouse anti-acetylated tubulin monoclonal antibody (1:1,000; Proteintech, USA), rabbit anti-H3K27me3 antibody (1:1,000; CST, USA), rabbit anti-NeuroD1 antibody (1:1,000; Abcam, USA), rabbit anti-UTX antibody (1:1,000; Abcam, USA), actin (1:5,000; Abcam, USA), rabbit anti-β-tubulin antibody (1:1,000; CST, USA), and anti-GAPDH antibody (1:5,000; Abcam, USA). After the membranes were washed with 1% Tris-HCl+Tween (TBST) 3 times (15 min each), they were incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:5,000; Proteintech, USA) for 90 min. Immunoreactive bands were visualized using enhanced chemiluminescence.

Guo Z., Li C., Cao Y., Qin T., Jiang L., Xu Y., Li M., Luo Z., Hu J, & Lu H. (2020). UTX/KDM6A deletion promotes the recovery of spinal cord injury by epigenetically triggering intrinsic neural regeneration. Molecular Therapy. Methods & Clinical Development, 20, 337-349.

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KPNB1 and NEUROD1 Immunoprecipitation

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Nuclear fractions from 4×10⁶ NCI-H2107, NCI-H889, NCI-H524, or NCI-H2171 cells were prepared (NE-PER™ Nuclear and Cytoplasmic Extraction kit, ThermoFisher #78835). 100 μg nuclear protein lysates from NCI-H2107 and -H889 cells were incubated overnight (4°C) with 5 μg mouse anti-KPNB1 antibody (Santa Cruz #sc-137016) and NCI-H524 and -H2171 lysates were incubated with 5 μg rabbit anti-NEUROD1 antibody (Abcam #ab109224). Normal mouse or rabbit IgG antibodies (Santa Cruz #sc-2025; Cell Signaling Technology #2729S) were used as respective controls. Immunoprecipitates were incubated for 90 minutes (4°C) with 70 μL Pierce™ Protein G Agarose beads (ThermoFisher #20397), washed in PBS containing 1% Triton-X100, eluted in Laemmli buffer, and resolved on 4–15% Mini- PROTEAN® TGX Stain- Free™ Protein Gels (Bio-Rad), transferred to PVDF membranes, and probed with mouse anti- ASCL1 antibody (1:1000, BD Biosciences #556604) or mouse anti-KPNB1 antibody (1:1000, Santa Cruz #sc-137016), respectively, followed by HRP-conjugated anti-mouse secondary antibody (1:10000, Invitrogen #31432) or HRP-conjugated Trueblot® anti-rabbit secondary antibody (1:1000, Rockland #18-8816-33).

Kelenis D.P., Rodarte K.E., Kollipara R.K., Pozo K., Choudhuri S.P., Spainhower K.B., Wait S.J., Stastny V., Oliver T.G, & Johnson J.E. (2022). Inhibition of karyopherin β1-mediated nuclear import disrupts oncogenic lineage-defining transcription factor activity in small cell lung cancer. Cancer research, 82(17), 3058-3073.

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