Cellular senescence was induced in proliferative UC-MSCs using two models: (1) DDIS: the UC-MSCs were cultured with DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin, 1% (v/v) streptomycin (all from Sigma-Aldrich, Madrid, Spain) and 1-μM Etoposide (MedChemexpress, New Jersey, USA) (Table A1 ) for two days. The cells were then washed with PBS and cultured with DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and 1% (v/v) streptomycin (all from Sigma-Aldrich, Madrid, Spain) for four days (Figure 2 A). (2) TIS: the UC-MSCs were cultured with DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin, 1% (v/v) streptomycin (all from Sigma-Aldrich, Madrid, Spain) and 1-μM Palbociclib (CDK4/6 inhibitor) (MedChemexpress, New Jersey NJ, USA) (Table A1 ) for three days. After that, the cells were washed with PBS and cultured with DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and 1% (v/v) streptomycin (all from Sigma-Aldrich, Madrid, Spain) for three days. The cells were then washed with PBS and cultured with DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and 1% (v/v) streptomycin (all from Sigma-Aldrich, Madrid, Spain) for three days (Figure 2 A).
Penicillin
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, France, Switzerland, Brazil, China, Poland, Macao, Spain, Canada, Japan, Australia, Austria, Belgium, Israel, Sao Tome and Principe, Netherlands, India, Sweden, Ireland, Argentina, Czechia, Denmark, New Zealand, Hungary, Mexico, Holy See (Vatican City State), Ukraine
Penicillin is a type of antibacterial drug that is widely used in medical and laboratory settings. It is a naturally occurring substance produced by certain fungi, and it is effective against a variety of bacterial infections. Penicillin works by inhibiting the growth and reproduction of bacteria, making it a valuable tool for researchers and medical professionals.
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Lab products found in correlation
Streptomycin, by Merck Group (6938 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1929 mentions)
L glutamine, by Merck Group (1166 mentions)
Fetal bovine serum (fbs), by Merck Group (1038 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (753 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (469 mentions)
Amphotericin b, by Merck Group (367 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (365 mentions)
Streptomycin, by Thermo Fisher Scientific (357 mentions)
Penicillin, by Thermo Fisher Scientific (349 mentions)
Rpmi 1640 medium, by Merck Group (346 mentions)
Rpmi 1640, by Merck Group (260 mentions)
Glutamine, by Merck Group (247 mentions)
L glutamine, by Thermo Fisher Scientific (242 mentions)
Rpmi 1640, by Thermo Fisher Scientific (227 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (222 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (204 mentions)
Fetal calf serum (fcs), by Merck Group (184 mentions)
Sodium pyruvate, by Merck Group (179 mentions)
Hepes, by Merck Group (174 mentions)
7 834 protocols using penicillin
1
Senescence Induction in UC-MSCs
2
Cell Line Cultivation Protocols
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NIH:OVCAR-3 cell line was grown in RPMI-1640 culture medium (Sigma-Aldrich, Germany), supplemented with 2 mM L-glutamine, 100 U/mL penicillin/100 µg/mL streptomycin (Sigma-Aldrich), 15% fetal bovine serum (FBS) (Sigma-Aldrich, Germany), and 0.01 mg/mL bovine insulin (Sigma-Aldrich). A2780 and A2780 Cis cell lines were cultivated in RPMI 1640 medium supplemented with 10% Fetal Bovine Serum (Sigma Aldrich), 2 mM L-glutamine, 1% antibiotics (penicillin + streptomycin) (all reagents from Sigma Aldrich) and with 1 µM cisplatinum in the case of A2780 Cis cell line. Ishikawa cell line was cultivated in Eagle’s Minimal Essential Medium (MEM) supplemented with 10% FBS, 2 mM L-glutamin, 1 mM sodium pyruvate, 1% NEA, and 1% antibiotics [25 (link)]. BJ HEP cell line was cultivated in Eagle’s Minimal Essential Medium (MEM), supplemented with 10% Fetal Bovine Serum (Sigma Aldrich), 2 mM L-glutamine, 1% antibiotics (penicillin + streptomycin), and 1% Non-Essential Aminoacids (NEA) Solution (Sigma Aldrich) [26 (link)]. HUVEC cell line was cultivated in RPMI medium, with 10% Fetal Bovine Serum (Sigma Aldrich), 2 mM L-glutamine, and 1% antibiotics (penicillin + streptomycin) (all reagents from Sigma Aldrich). Cell cultures in the 12th passage were used [27 (link)].
3
Prostate Cancer and Mesenchymal Stem Cell Co-culture
4
Cell Culture Protocols for Diverse Cell Lines
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Human embryonic kidney (HEK) 293T cells (GenHunter Co., Nashville, TN) were maintained in Dulbecco's Modified Eagle's Medium (DMEM)(Sigma-Aldrich) containing 1.0 g/L glucose, 2 mM L-glutamine (Sigma-Aldrich), 100 IU/mL penicillin (Sigma-Aldrich), 0.1 µg/mL streptomycin (Sigma-Aldrich) and 10% fetal bovine serum (FBS) (HyClone). Three human neuronal cell lines, neuroepithelioma cells (HTB-10, or SK-N-MC), neuroblastoma cells (HTB-11 or SK-N-SH, and SH-SY5Y), and glioblastoma cells (HTB-14; or U-87) (ATCC, Manassas, VA), were cultured in Minimum Essential Medium (Eagle) (MEM) (Sigma-Aldrich) supplemented with 2 mM L-glutamine, 1.0 mM sodium pyruvate, 100 IU/mL penicillin, 0.1 µg/mL streptomycin and 10% FBS. The human embryonic microglial cell line CHME-5 (provided by Dr. Pierre Talbot, Universite du Quebec) was cultured in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma-Aldrich) containing 4.5 g/L glucose, 2 mM L-glutamine, 100 IU/mL penicillin, 0.1 µg/mL streptomycin and 10% FBS. Human monocytic cell line U937 (ATCC Manassas, VA), was cultured in RPMI1640 (Sigma- Aldrich) supplemented with 2 mM L-glutamine, 1.0 mM sodium pyruvate, 100 IU/mL penicillin, 0.1 µg/mL streptomycin and 10% FBS.
5
Inducible Reprogramming of Diverse Cell Lines
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HEK293T, SNL, and C57BL/6 primary MEFs carrying a doxycycline-inducible tetracistronic cassette encoding the four murine reprogramming factors Oct4, Sox2, Klf4, and c-Myc (provided by M. Serrano) (Abad et al., 2013 (link)) were grown in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), 100 units/mL penicillin and 100 g/mL streptomycin (Sigma-Aldrich). HFF cells were growth in DMEM (Gibco) supplemented with 20% FBS (ATCC, LGC Promochem) and 100 units/mL penicillin (Sigma-Aldrich). miPSCs were cultured on gelatin-coated plates with DMEM/F12 + GlutaMAX (Gibco) supplemented with 20% knockout serum replacement (Gibco), 1,000 U/mL LIF (Millipore), 1% non-essential amino acids, 0.1 mM β-mercaptoethanol, and 100 units/mL penicillin (Sigma-Aldrich). hiPSCs were cultured on SNL feeders with DMEM/F12 + GlutaMAX (Gibco) supplemented with 20% knockout serum replacement (Gibco), 10 ng/mL basic fibroblast growth factor (Miltenyi Biotec), 1% non-essential amino acids, 0.1 mM β-mercaptoethanol, and 100 units/mL penicillin (Sigma-Aldrich).
6
Cell Culture Protocols for Various Cell Types
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Culture of fibroblasts was performed in Dulbecco-Vogt modification of Eagle’s medium (DMEM, Invitrogen, Waltham, Massachusetts, USA) supplemented with 10% Fetal Bovine Serum (FBS) (Hyclone, GE Healthcare, Little Chalfont, United Kingdom), 100 U/mL penicillin (Sigma-Aldrich, Saint-Louis, MO, USA) and 25 µg/mL gentamicin (Schering, Kenilworth, New Jersey, USA). Keratinocytes were grown in co-culture with irradiated 3T3 murine fibroblasts in a 3:1 DMEM-Ham’s F12 medium supplemented with 5% newborn calf serum (FetalClone II, HyClone), 10−10 M cholera toxin (Sigma-Aldrich, Saint-Louis, Missouri, USA), 5 mg/mL insulin (Sigma-Aldrich), 10 ng/mL human epidermal growth factor (Austral Biologicals, San Ramon, CA), 0.4 mg/mL hydrocortisone (Calbiochem, San Diego, CA, USA) and 100 U/mL penicillin (Sigma-Aldrich) and 25 µg/mL gentamicin (Schering). HMVEC were grown in EGM-2MV medium (Lonza, Basel, Switzerland). A375, RPMI 7951, SK MEL-28 and Malme 3 M were amplified in complete DMEM media like the fibroblasts, whereas WM983A and WM983B cells were cultured in RPMI 1640 supplemented with 10% FBS (Hyclone), 100 U/mL penicillin (Sigma-Aldrich) and 25 µg/mL gentamicin (Schering).
7
Culturing Human Cell Lines and Isolating Neutrophils
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HMEC-1 cells (ATCC® CRL-3243TM, USA) were cultured in complete MCDB 131 medium (Thermofisher Scientific, Horsham, UK) containing 10% foetal bovine serum (FBS, Lonza, Basel; Switzerland), L-glutamine dissolved in sterile water supplemented with 15.7 mL/L sodium bicarbonate solution (7.5% w/v), 10 ng/mL epidermal growth factor (EGF); 1 μg/mL hydrocortisone, 100 μg/mL penicillin and 0.1 mg/mL streptomycin (all Sigma Aldrich, Dorset, UK). HUVECs were cultured in complete endothelium growth medium (ATCC, Virginia, USA) containing Endothelial Cell Growth Kit-BBE (ATCC, USA). Human colorectal adenocarcinoma cells (Caco-2, ATCC® HTB-37TM, USA) were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma Aldrich, USA) containing 10% FBS, L-glutamine dissolved in sterile water with 15.7 mL/L sodium bicarbonate solution (7.5% w/v), 100 μg/mL penicillin and 0.1 mg/mL streptomycin (all Sigma Aldrich, USA). Human primary neutrophils were isolated using Percoll density gradient and suspended in RPMI-1640 medium with 0.5% fatty-acid-free BSA, 2 mM L-glutamine, 100 U/mL penicillin and 0.1 mg/mL streptomycin (all Sigma Aldrich, USA).
8
Maintenance of Myeloma and Lymphoma Cell Lines
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The SP2/0-Ag14 and SP2/0-NS3/4A myeloma cell lines (H-2d)22 (link) was maintained in Dulbecco's modifed Eagle's medium medium supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, St Louis, MO, USA), 2 mM L-glutamine, 10 mM HEPES, 100 U ml−1 penicillin, 100 μg ml−1 streptomycin, 1 mM nonessential amino acids, 50 μM β-mercaptoethanol and 1 mM sodium pyruvate (Sigma Aldrich). SP2/0-Ag14 cells with stable expression of NS3/4A were maintained in 800 μg geneticin (G418) per ml complete Dulbecco's modifed Eagle's medium.
The EL-4 and EL-4-NS3/4A lymphoma cell lines (H-2b)17 (link) were maintained in RPMI 1640 medium supplemented with 10% FBS, 10 mM HEPES, 1 mM sodium pyruvate, 1 mM nonessential amino acids, 50 μM β-mercaptoethanol, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin (Sigma Aldrich). EL-4 cells with stable expression of NS3/4A were maintained in 800 μg geneticin (G418) per ml complete RPMI 1640 medium.
RMA-S cells (H-2Kd, H-2Dd or H-2Ld) were maintained in RPMI 1640 medium supplemented with 5% FBS, 2 mM L-glutamine, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin (Sigma Aldrich). All cells were grown in a humidified 37 °C and 5% CO2 incubator.
The EL-4 and EL-4-NS3/4A lymphoma cell lines (H-2b)17 (link) were maintained in RPMI 1640 medium supplemented with 10% FBS, 10 m
RMA-S cells (H-2Kd, H-2Dd or H-2Ld) were maintained in RPMI 1640 medium supplemented with 5% FBS, 2 m
9
Characterizing miRNA Regulation in Leukemia
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The human promyelocytic cell line NB4 and human embryonic kidney cell line HEK-293T were cultured in RPMI-1640 medium (Gibco, BRL, UK) supplemented with 10% FCS (Gibco), 50 U/ml penicillin, and 50 mg/ml streptomycin (Sigma, St. Louis, MO, USA) at 37°C in 5% CO2. The human promyelocytic cell line HL-60 was cultured in IMDM medium (Gibco) supplemented with 10% FCS, 50 U/ml penicillin, and 50 mg/ml streptomycin (Sigma) at 37°C in 5% CO2. The lentivirus packaging cell line HEK-293TN was maintained in DMEM medium supplemented with 10% FCS, 50 U/ml penicillin, and 50 mg/ml streptomycin (Sigma). HEK-293T cell line was only used for dual-luciferase reporter assay and packaging lentivirus; while NB4 and HL-60 AML cells were used to identify the function of miR-451, YWHAZ, c-Myc and HDAC3.
10
Propagation of HSV-1 and Bovine Coronavirus in Cell Cultures
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HSV-1 McKrae [27 (link)] and HSV-1-GFP [28 (link)] were cultured in Vero cells in Eagle’s Minimum Essential Medium (EMEM) supplemented with 5% fetal bovine serum (FBS) (GeminiBio, Sacramento, CA, USA), penicillin (100 U/mL), and streptomycin (100 μg/mL) (Sigma-Aldrich, Inc., St. Louis, MO, USA). Vero cells were cultured in EMEM supplemented with 10% fetal bovine serum (FBS) (GeminiBio, USA), penicillin (100 U/mL), and streptomycin (100 μg/mL) (Sigma-Aldrich, Inc., St. Louis, MO, USA) at 37 °C with 5% CO2 in a humidified incubator as we described previously [22 (link),29 (link)].
Bovine coronavirus (BCoV) was the NADL-Nebraska Strain isolated by the National Veterinary Services Laboratories (NVSL) in 1981 [30 (link)]. BCoV was grown on human rectal tumor (Hrt-18G) cells [30 (link),31 (link)], which were maintained in EMEM supplemented with 10% FBS (GeminiBio, USA), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Sigma-Aldrich, Inc.) at 37 °C with 5% CO2 in a humidified incubator. The virus was propagated in EMEM supplemented with 2.5 μg/mL trypsin and 2.5 μg/mL pancreatin, 1× insulin–transferrin–selenium (Cat No. 51500-056, GIBCO) [31 (link)].
Bovine coronavirus (BCoV) was the NADL-Nebraska Strain isolated by the National Veterinary Services Laboratories (NVSL) in 1981 [30 (link)]. BCoV was grown on human rectal tumor (Hrt-18G) cells [30 (link),31 (link)], which were maintained in EMEM supplemented with 10% FBS (GeminiBio, USA), penicillin (100 U/mL) and streptomycin (100 μg/mL) (Sigma-Aldrich, Inc.) at 37 °C with 5% CO2 in a humidified incubator. The virus was propagated in EMEM supplemented with 2.5 μg/mL trypsin and 2.5 μg/mL pancreatin, 1× insulin–transferrin–selenium (Cat No. 51500-056, GIBCO) [31 (link)].
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