Facs calibur cell sorter

Manufactured by BD

Sourced in United States

The BD FACS Calibur cell sorter is a flow cytometry instrument designed for cell analysis and sorting. It is capable of detecting and measuring multiple parameters of individual cells within a sample, including size, granularity, and fluorescence. The FACS Calibur provides users with the ability to identify and isolate specific cell populations of interest.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor conjugated foxp3 antibody, by Miltenyi Biotec (3 mentions) Cellquest software, by BD (2 mentions) Facs permeabilizing solution 2, by BD (2 mentions) Cellrox green reagent, by Thermo Fisher Scientific (2 mentions) Green filters, by Leica (1 mentions) Cellquest software version 3, by BD (1 mentions) Facsdiva 8, by BD (1 mentions) Apc anti human cd56, by BD (1 mentions) Anti human cd8 pe, by BD (1 mentions) Cell staining buffer, by BioLegend (1 mentions) Percp anti human cd95, by BD (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Phycoerythrin conjugated anti cd133 1 antibody, by Miltenyi Biotec (1 mentions) Recombinant murine granulocyte macrophage colony stimulating factor, by Thermo Fisher Scientific (1 mentions) Rneasy kit, by Qiagen (1 mentions) Doxycycline, by Merck Group (1 mentions) α amanitin, by Merck Group (1 mentions) Aphidicolin, by Merck Group (1 mentions) 4 oht, by Merck Group (1 mentions) Recombinant human il 2, by R&D Systems (1 mentions)

Spelling variants of this product

Calibur (250 citations) BD FACSTM (2 citations)

30 protocols using facs calibur cell sorter

Lentiviral Transduction Efficiency Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Expression lentiviral particles were produced in 293 T cells in DMEM medium according to the manufacturer’s protocol. The viruses were collected and filtered through 0.45-μm filters. Virus titers were measured via GFP-positive cells after transduction into TH1080 cells; the concentrations of the titers were 1.25 × 10⁷ IFU/mL for Mock and 1.15 × 10⁷ IFU/mL for GLS2.
The T98G, U87MG and LN18 cell lines were transduced with the corresponding lentiviruses for 24 hours in the presence of 4 μg/mL polybrene. The transduced cells were selected using puromycin (Cat. No. A7793, Sigma-Aldrich, St Louis, MO, USA), and the transduction efficacy was observed by fluorescence microscopy using green filters (Leica, Wetzlar, Germany). The transduction efficiency of each cell line expressing GFP was over 90%, and then the cells were analyzed using a FACS Calibur cell sorter (BD Bioscience, San Jose, CA, USA) equipped with a 530-nm filter (bandwidth, 15 nm), a 585-nm filter (bandwidth, 21 nm), and Cell-Quest software (BD Bioscience, San Jose, CA).

Kim S., Kim J.E., Kim Y.H., Hwang T., Kim S.K., Xu W.J., Shin J.Y., Kim J.I., Choi H., Kim H.C., Cho H.R., Choi A., Chowdhury T., Seo Y., Dho Y.S., Kim J.W., Kim D.G., Park S.H., Kim H., Choi S.H., Park S., Lee S.H, & Park C.K. (2017). Glutaminase 2 expression is associated with regional heterogeneity of 5-aminolevulinic acid fluorescence in glioblastoma. Scientific Reports, 7, 12221.

+ Open protocol

+ Expand

Cytotoxicity Assessment of Luc-loaded Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Luc alone and Luc-loaded O-GNR-PEG-DSPEs at different concentrations (5–80 μM) were incubated with CMV/U251, A1-5/CMV/U251 and CG-4 (5 × 10⁵ cells/3ml in 6-well plates) for 4 h. Unexposed cells were used as control. Unloaded O-GNR-PEG-DSPEs in amounts equivalent to the loaded nanoparticles were also exposed to all the cell types as delivery agent control. The viability of the cells was analyzed by flow cytometry after Annexin V/PI staining using FACS Calibur Cell Sorter (BD Biosciences, San Jose, CA).

Chowdhury S.M., Surhland C., Sanchez Z., Chaudhary P., Kumar M.A., Lee S., Peña L.A., Waring M., Sitharaman B, & Naidu M. (2014). Graphene Nanoribbons as a Drug Delivery Agent for Lucanthone Mediated Therapy of Glioblastoma Multiforme. Nanomedicine : nanotechnology, biology, and medicine, 11(1), 109-118.

+ Open protocol

+ Expand

Apoptosis Detection by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

According to the published protocols, flow cytometry was used to detect cell apoptosis. Cells were collected after corresponding treatments and centrifugation for 5 min at 1,000 rpm. PI (Propidium iodide; 10 μl) and FITC (fluorescein isothiocyanate)-Annexin V (5 μl) were added into the cells (100 5 μl) followed by incubation for a quarter in darkness at room temperature. The cells were immediately analyzed after the aforementioned treatment by using the flow cytometer. FACS Calibur cell sorter (BD FASAria Cell Sorter) was used in this assay. Three independent experiments were conducted and triplicated samples were used.

Qu Y., Cao J., Wang D., Wang S., Li Y, & Zhu Y. (2022). 14,15-Epoxyeicosatrienoic Acid Protect Against Glucose Deprivation and Reperfusion-Induced Cerebral Microvascular Endothelial Cells Injury by Modulating Mitochondrial Autophagy via SIRT1/FOXO3a Signaling Pathway and TSPO Protein. Frontiers in Cellular Neuroscience, 16, 888836.

+ Open protocol

+ Expand

Cell Cycle Arrest Analysis of SKOV-3 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

SKOV-3 cells (5 × 10⁴) were placed in 500 μl of complete media seeded into 6-well plates and treated with Fe₃O₄ and CS-Fe₃O₄ (10 μg/ml) at different pH values for 48 h before performing cell cycle arrest analysis. The cells were harvested via centrifugation at 1,000 rpm for 5 min at room temperature, washed with ice-cold phosphate-buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 2 mM KH₂PO₄ pH 7.2) three times, then fixed with 70% ethanol at 4°C overnight. Fixed cells were treated with 100 μg/ml propidium iodide (PI) and 100 μg/ml RNase A in PBS and incubated at room temperature for 25 min. Finally, cell cycle arrest was analyzed using a FACSCalibur cell sorter (BD Biosciences, Franklin Lakes, NJ, United States) and CellQuest software version 3.3 (BD Biosciences) according to the manufacturer’s instructions.

Zhang T., Wang L., He X., Lu H, & Gao L. (2022). Cytocompatibility of pH-sensitive, chitosan-coated Fe3O4 nanoparticles in gynecological cells. Frontiers in Medicine, 9, 799145.

+ Open protocol

+ Expand

Prostate Cancer Stem Cell Profiling

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

FACS analysis for prostate cancer stem cells was based on prior publications (1 (link),19 (link),21 (link)-23 (link)). Before labeling, the cells were blocked with normal mouse IgG in 1:100 dilution for 30 min and then incubated with fluorescein isothiocyanate (FITC) or phycoerythrin (PE)-labeled rat anti mouse Sca-1 (clone E13-161.7, Phamingen) (1/100-1/200), PE-labeled rat anti-mouseCD133 (1:10) (clone MB9-3G8, Miltenyl Biotec), PE/Cy5-labeled rat anti-human/mouse CD44 (1:200) (clone IM7, BioLegend, San Diego), PE/Cy5-labeled rat anti-human/mouse CD49f (1/10) and/or FITC-lebeled goat anti mouse Trop2 (FAB1122F, R&D) for 1 h. All experiments were conducted at 4°C. Cell sorting was performed on a FACS Calibur cell sorter (BD Biosciences). The data were analyzed with FlowJo software (Tree Star, Inc., Ashland, OR).

Ju X., Jiao X., Ertel A., Casimiro M.C., Di Sante G., Deng S., Li Z., Di Rocco A., Zhan T., Hawkins A., Stoyanova T., Ando S., Fatatis A., Lisanti M.P., Gomella L.G., Languino L.R, & Pestell R.G. (2016). SRC ONCOGENE INDUCES TROP2 PROTEOLYTIC ACTIVATION VIA CYCLIN D1. Cancer research, 76(22), 6723-6734.

+ Open protocol

+ Expand

Annexin V Apoptosis Assay for RGCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

RGCs were trypsinized and washed gently with PBS, and then resuspended in 1 × Binding Buffer. Fluorescein isothiocyanate Annexin Ⅴ (5 μL) and PI (5 μL) (556,457; BD Biosciences) were added to 100 μL of cell suspension (1 × 10⁶ cells/mL). Cells were subsequently incubated for 15 min at RT in the dark. Following incubation, we added 400 μL 1 × Binding Buffer. A FACSCalibur Cell Sorter (BD Biosciences) was used to detect cell apoptosis and data were analyzed by FlowJo V10 software.

Ge Y., Zhang R., Feng Y., Lu J, & Li H. (2021). Mbd2 deficiency alleviates retinal cell apoptosisvia the miR-345-5p/Atf1 axis in high glucoseinjury and streptozotocin-induced diabetic mice. Molecular Therapy. Nucleic Acids, 26, 1201-1214.

+ Open protocol

+ Expand

EGFR Expression Quantification by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was used toquantify the activated EGFR expression before and after siRNA transfection (against EGFR and HPV18 E5), and the transfection efficiency and expression of the MSCV-FLAG-HPV16 E5 plasmid in A431 and C33A cells. To prepare for flow cytometry, the cells were fixed using 2.5% glutaraldehyde, treated with 0.1% Triton X 100 for 5 minutes. Next, the cells were treated with appropriate antibodies, trypsinized, and resuspended in FACS buffer (Phosphate buffered saline containing 20% fetal bovine serum). Flow cytometry was performed immediately after all samples were prepared using a FACS Calibur Cell Sorter (BD Biosciences, San Jose, CA). BD FACS Diva 8.0 software was used for data analysis.

Chowdhury S.M., Mannepalli P, & Sitharaman B. (2014). Graphene Nanoribbons Elicit Cell Specific Uptake and Delivery Via Activation of Epidermal Growth Factor Receptor Enhanced by Human PapillomaVirus E5 Protein. Acta biomaterialia, 10(10), 4494-4504.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

After isolation of PBMCs, the CD8⁺ T cells were stained with PE anti-human CD8 (BD Biosciences) and PerCP anti-human CD95 (BD Biosciences) antibodies for 30 min in the dark. NK cells were stained with PE anti-human CD16 (BD Bioscience), APC anti-human CD56 (BD Biosciences), and PerCP anti-human CD95 (BD Biosciences) antibodies for 30 min in the dark. After staining, the cells were washed with cell staining buffer (Biolegend, USA) and stained using FITC anti-human annexin-V (Biolegend) antibody for 30 min in the dark. After staining, the cells were fixed with 4% paraformaldehyde at room temperature for 10 min in the dark. All data were collected using a FACSCalibur cell sorter (BD Biosciences, USA). Data were analysed using the FlowJo software (ver. 10.2; Tree Star Inc., [Ashland/OR] USA).

Lee J.Y., Seo E.H., Oh C.S., Paik J.H., Hwang D.Y., Lee S.H, & Kim S.H. (2017). Impact Of Circulating T Helper 1 And 17 Cells in the Blood on Regional Lymph Node Invasion in Colorectal Cancer. Journal of Cancer, 8(7), 1249-1254.

+ Open protocol

+ Expand

CD133 Expression in Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were harvested with 0.5 mM trypsin/EDTA (Invitrogen), incubated at 4 °C with phycoerythrin-conjugated anti-CD133/1 antibody (Miltenyi Biotec, Auburn, CA, USA), and analyzed with a FACSCalibur cell sorter (BD Biosciences, Pharmingen, San Jose, CA, USA). The CD133⁺ and CD133^– cells were sorted using the FACSCalibur cell sorter. Isotype-matched mouse IgG was used as a control.

Lee Y.K., Hur W., Lee S.W., Hong S.W., Kim S.W., Choi J.E, & Yoon S.K. (2014). Knockdown of 14-3-3ζ enhances radiosensitivity and radio-induced apoptosis in CD133+ liver cancer stem cells. Experimental & Molecular Medicine, 46(2), e77-.

+ Open protocol

+ Expand

Cellular Uptake of PI-loaded O-GNR-PEG-DSPEs

Check if the same lab product or an alternative is used in the 5 most similar protocols

O-GNR-PEG-DSPEs were loaded with PI and purified using the same method used for Luc loading. CMV/U251 and A1-5/CMV/U251 were grown in 10 cm dishes at 37 °C and 5% CO₂ in DMEM. Cells were either incubated with PI-loaded O-GNR-PEG-DSPEs at a concentration of 40 μg per mL (previously reported to be a non-toxic concentration) (15) of media, or left untreated. After 24 h, cells were trypsinized, resuspended in FACS buffer (1X PBS containing 20% fetal bovine serum) and placed on ice. Flow cytometry was performed immediately after all samples were prepared using a FACS Calibur Cell Sorter (BD Biosciences, San Jose, CA).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!