All human fibroblasts from Parkinson patients and healthy control individuals are from the Blood- and Tissue-library Hertie Institute for Clinical Brain Research Tuebingen (Ethics-Application 287/2004V). Human fibroblasts are cultured in RPMI media (Millipore, F1215) containing 10% (v/v) FCS (Gibco by life technologies, 10270), 1 mM Sodium pyruvate (Millipore, L0473) and 1x Glutamax-I (Gibco by life technologies, 35050-038).
Sodium pyruvate
Sodium pyruvate is a chemical compound that functions as an energy source and metabolic intermediate in cell culture media. It is commonly used as a supplement in cell culture applications to support cell growth and metabolism.
Lab products found in correlation
1 753 protocols using sodium pyruvate
Isolation and Culture of Mammalian Fibroblasts
All human fibroblasts from Parkinson patients and healthy control individuals are from the Blood- and Tissue-library Hertie Institute for Clinical Brain Research Tuebingen (Ethics-Application 287/2004V). Human fibroblasts are cultured in RPMI media (Millipore, F1215) containing 10% (v/v) FCS (Gibco by life technologies, 10270), 1 mM Sodium pyruvate (Millipore, L0473) and 1x Glutamax-I (Gibco by life technologies, 35050-038).
Metabolic Tolerance Tests in Mice
Hydrogen Peroxide Scavenging Assay
In the assay the sample is mixed with an aliquot of 2 mM H2O2, 2.56 mM ammonium iron (II) sulphate·6H2O and 111 µM xylenol orange disodium salt and after 30 min incubation, the absorbance of ferric-xylenol orange complex at 560 nm was measured.) Sodium pyruvate (Sigma-Aldrich Chemie GmbH, St. Louis, MO, USA) was used as a reference compound. Reagents: H2O2 (Merck KGaA, Darmstadt, Germany), ammonium iron (II) sulphate·6H2O (BDH Prolabo, Dubai, UAE), xylenol orange disodium salt (Sigma-Aldrich Chemie GmbH, St. Louis, MO, USA), Sodium pyruvate (Sigma-Aldrich Chemie GmbH, St. Louis, MO, USA).
Cell Culture Conditions for BS153 and DKMG
Cultivation of Cancer Cell Lines
Casein-Casein-Yeast Medium for PVL Production
Modulating Oxidative Phosphorylation in Cells
Chondrogenic and Hypertrophic Differentiation of BMSCs
Propagation and Maintenance of H1299-E3 and VeroE6 Cell Lines
Culturing Oral Cancer Cell Lines
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