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5 protocols using sodium lignosulfonate

1

Cationic Polymer-Based Dye Adsorption

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Sodium lignosulfonate (C20H24Na2O10S2,
MW 534.51 g/mol, 96%), chitosan (C6nH11nNO4n, 95% deacetylated,
viscosity 100–200 mPa s), Rhodamine B (C28H31ClN2O3, MW 479.01 g/mol, AR), and Congo
red (C32H22N6Na2O6S2, MW 696.66 g/mol, BS) were obtained from Shanghai
Macklin Biochemical Co., Ltd. Potassium dichromate (K2Cr2O7, MW 294.18 g/mol, GR) was provided by Sinopharm
Chemical Reagent Co., Ltd. Acetic acid (CH3COOH, MW 60.05
g/mol, AR) was purchased from Beijing Chemical Works. In addition,
all other solutions were prepared by distilled water. All chemicals
in the experiments were used without further purification.
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2

Platinum-based Catalytic Conversion Protocol

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Hexachloroplatinic acid (H2PtCl6·6H2O) and potassium chloroplatinate (K2PtCl6·6H2O) were purchased from
Aladdin. Sodium lignosulfonate was purchased from Macklin and dried
in a vacuum oven for 24 h before use. Sodium type 732 cation exchange
resins were purchased from Sinopharm. α-Pinene was purchased
from Jiangxi Hessence Chemicals Co., Ltd., China. The remaining reagents
used in the catalytic tests were purchased from Macklin and were used
without any further treatment.
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3

Lignocellulosic Biomass Pretreatment Protocol

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Sodium lignosulfonate (96%, 534.15 MW) was purchased from Shanghai Macklin Biochemical Ltd (China). Sulfuric acid (AR), hydrochloric acid and hydrogen peroxide (30 wt%, AR) were obtained from Guangzhou Chemical Reagent Factory (China). d-Xylose (AR) and calcium carbonate (AR) were purchased from Tianjin Fuchen Chemical Reagent Factory (China). All reagents were used without purification. Corncob powder was acquired from a farm in Shandong Province, China. It was ground into particles with a size of 40–60 mesh and then oven-dried at 80 °C for 12 h.
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4

Bamboo-Based Biomaterial Fabrication

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Selected 60–100 mesh wood natural bamboo (Phyllostachys heterocycle) (China Guangdong Shenglong Bamboo & Wood Co., Ltd., Guangzhou, China) was used as the experimental raw material. Sodium hydroxide (≥96%) was used in the experiment and was purchased from Sinopharm Reagent Group Co., Ltd. (Shanghai, China) Chitosan, deacetylation degree ≥ 95%, was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China) and sodium lignosulfonate was purchased from Shanghai Macklin Biochemical Technology Co., Ltd.
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5

Formulation and Analysis of Fluopyram

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Fluopyram (97.5% purity) was purchased from Bayer (China) Co., Ltd. (Leverkusen, Germany). 1-Dodecanesulfonic acid sodium salt (SDS), sodium dodecyl benzene sulfonate (SDBS), sodium lignosulfonate (SL, methoxy group: 5~7%, carbon: 45~47%), hydroxypropyl methyl cellulose (HPMC, hydroxylpropoxyl: 4~12%, methoxy: 19~24%, 100 mPa.s), sodium salt of polynaphthalene sulphonic acid (NNO), white carbon, diatomite, glucose, sucrose, mannitol, anhydrous d-trehalose, and sorbitol were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Pluronic F127 was obtained from Sigma-Aldrich (Shanghai, China). Polycarboxylate (TERSPERSE 2700) was provided by Huntsman Technology Corporation (USA). Morwet D425 and Morwet EFW were provided by Nanjing Jierun Technology Co., Ltd. (Nanjing, China). LC/MS-grade and HPLC-grade methanol and acetonitrile were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Ultrapure water was used in all experiments. Qiagen RLT Lysis solution was purchased from Beijing Huaxia Ocean Technology Co., Ltd. (Beijing, China).
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