Accu chek performa

Manufactured by Roche

Sourced in Germany, United States, Switzerland, Australia, France, Brazil, China, Japan, Argentina, Thailand, United Kingdom, Canada

The Accu-Chek Performa is a blood glucose monitoring system designed to measure the level of glucose in the user's blood. It provides accurate and reliable readings to help individuals manage their diabetes.

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Close spelling variants of this product

Accu-Chek (690 citations) Accu-Chek Performa Nano (27 citations) Accu-Chek Performa Glucometer (25 citations) Accu-Chek Performa System (13 citations) Accu-Chek Performa II (11 citations) Accu-Chek Performa blood glucose meter (10 citations) Accu-chek Performa test strips (6 citations) ACCU-CHEK® Performa Kit (4 citations) Accu-Chek Performa Nano System (4 citations) Accu-Chek Performa Strips and Glucometer (4 citations)

477 protocols using accu chek performa

Glucose and Insulin Tolerance Tests

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OGTT and ITT were performed after the mice had received the test diet for 11 weeks at 19 weeks of age. After 8 h of fasting, the baseline blood glucose level was measured by tail vein puncture. For OGTT, a solution of 40% glucose (2 g/kg body weight) was administered by oral gavage. After glucose administration, blood samples were collected from the tail vein at 15, 30, 60, 90, and 120 min. Blood glucose levels were measured using Accu-Chek Performa glucometer (Boehringer-Mannheim, Indianapolis, IN, USA).
For ITT, the mice were fasted for 8 h under nonanesthetized conditions. Insulin-R (I9278, Sigma-Aldrich, St. Louis, MO, USA) was intraperitoneally injected (0.75 U/kg body weight) and blood samples from the tail vein were collected at 15, 30, 60, 90, and 120 min after insulin injection. Glucose levels were evaluated with Accu-Chek Performa glucometer (Boehringer-Mannheim, Indianapolis, IN, USA).

Cho Y., Lee H., Park H.K., Choe E.Y., Wang H.J., Kim R.H., Kim Y, & Kang E.S. (2020). Differential Diabetogenic Effect of Pitavastatin and Rosuvastatin, in vitro and in vivo. Journal of Atherosclerosis and Thrombosis, 27(5), 429-440.

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Glucose and Insulin Metabolism Assays

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INS-1 b-cells were transfected with shRNA of mouse Erg2 (OriGene) or scramble shRNA (as a negative control) using Lipofectamine 2000 (Invitrogen). The target sequences are listed as follows: 3 0 -ACACGCAGATGCTGCGTGTCAAGGAGTTC-5 0 .
Intraperitoneal Glucose tolerance test (IPGTT), intraperitoneal insulin releasing test (IPIRT) and Insulin tolerance test (ITT) IPGTT and IPIRT were performed in mice after they fasted overnight for 16 has described previously (Niu et al., 2008) . At 0, 15, 30, 60, and 120min after glucose (2g/kg body weight for IPGTT or 3g/kg body weight for IPIRT) injection. For IPGTT about 1 mL blood sample was taken from the angular vein for testing blood glucose concentration using an automatic glucometer ACCU-CHEKâ Performa (Roche). For IPIRT about 20 mL blood sample was taken from the angular vein for testing insulin concentrations during IPGTT. Insulin concentration was assayed by an ELISA kit (Millipore). GLP-1 and glucagon were detected using ELISA kit (Millipore and R&D) according to the manufacturer's protocol.
ITT was performed in mice after fasted for 4h. At 0,15, 30, 60, 90, and 120 min after Human regular insulin (Eli Lilly) (0.1 U/ml in saline; 0.75 U/kg body weight) injection, about 1 mL blood sample was taken from the angular vein for testing blood glucose concentration using an automatic glucometer ACCU-CHEKâ Performa (Roche).

Yang J.K., Lu J., Yuan S.S., A.s.a.n., Cao X., Qiu H.Y., Shi T.T., Yang F.Y., Li Q., Liu C.P., Wu Q., Wang Y.H., Huang H.X., Kayoumu A., Feng J.P., Xie R.R., Zhu X.R., Liu C., Yang G.R., Zhang M.R., Xie C.L., Chen C., Zhang B., Liu G., Zhang X.Q, & Xu A. (2018). From Hyper- to Hypoinsulinemia and Diabetes: Effect of KCNH6 on Insulin Secretion. Cell reports, 25(13).

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Glucose Homeostasis Assessment in Mice

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The insulin tolerance test (ITT) was performed four days before euthanasia. Animals were bled at the tail vein and glucose levels were measured by a glucometer (Accu-Chek Performa; Roche, Diagnostics, USA) before, and 15, 30 and 60 minutes after 0.75 U/kg insulin injection i.p. Two days before euthanasia, oral glucose tolerance test (OGTT) was carried out after 6 hours of fasting. Mice were given glucose (2g/kg of body weight) by gavage and blood glucose levels were measured by a glucometer (Accu-Chek Performa; Roche) before, and 15, 30, 60 and 120 minutes after gavage.

Queiroz-Glauss C.P., Vieira M.S., Gonçalves-Pereira M.H., Almeida S.S., Freire R.H., Gomes M.A., Alvarez-Leite J.I, & Santiago H.C. (2022). Helminth infection modulates number and function of adipose tissue Tregs in high fat diet-induced obesity. PLoS Neglected Tropical Diseases, 16(5), e0010105.

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Intraperitoneal Glucose and Insulin Tolerance Tests

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For intraperitoneal glucose tolerance tests (ipGTT), mice were fasted for 16 h overnight. Subsequently, 5 µl of a 20% glucose solution (Glucosteril, Fresenius Kabi) per g body weight were injected intraperitoneally. Blood was withdrawn from the tail vein and glucose concentrations were measured before and 20, 40, 60, and 120 min after glucose application (ACCU-CHEK Performa, Roche).
For insulin tolerance tests (ITT), mice were fasted for 6 h before intraperitoneal injection of 1 mU human insulin/g body weight (Insuman Rapid, Sanofi). Blood glucose levels were measured from tail vein blood before and 15, 30, 45, and 60 min after insulin application (ACCU-CHEK Performa, Roche).

Dietzsch A.N., Al-Hasani H., Altschmied J., Bottermann K., Brendler J., Haendeler J., Horn S., Kaczmarek I., Körner A., Krause K., Landgraf K., Le Duc D., Lehmann L., Lehr S., Pick S., Ricken A., Schnorr R., Schulz A., Strnadová M., Velluva A., Zabri H., Schöneberg T., Thor D, & Prömel S. (2024). Dysfunction of the adhesion G protein-coupled receptor latrophilin 1 (ADGRL1/LPHN1) increases the risk of obesity. Signal Transduction and Targeted Therapy, 9, 103.

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Assessing Glucose Tolerance in Dams and Offspring

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IPGTTs were performed after an overnight fast on dams at the end of lactation as well as on the offspring at 3wks and 12wks of age. Baseline blood samples were collected from the tail vein and a glucose bolus (2g/kg of 50% dextrose in sterile 0.9% saline) was then injected intraperitoneally. Blood samples were collected from the tail vein at 5, 10, 15, 30, 60 and 120 minutes following glucose delivery. Glucose concentrations were determined using a handheld Accu-Chek Performa glucometer (Accu-Chek Performa©, Roche, Germany) at each timepoint.

Gugusheff J., Sim P., Kheng A., Gentili S., Al-Nussairawi M., Brand-Miller J, & Muhlhausler B. (2016). The effect of maternal and post-weaning low and high glycaemic index diets on glucose tolerance, fat deposition and hepatic function in rat offspring. Journal of developmental origins of health and disease, 7(3).

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Glycemic Index of Plantain Dishes

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The study was performed in accordance with the international standard GI testing protocol [14 ], in line with procedures recommended by the FAO/WHO Expert Consultation [13 ]. Subjects were invited to attend the test sessions on seven consecutive occasions with a 2-day interval between test days. Fifty (50) grams of anhydrous glucose powder dissolved in 250 mL water was used twice as the reference food; plantain dishes were prepared on the day of testing following the common practices used by the local food sellers in Côte d’Ivoire. All foods were tested in portions containing equivalent available carbohydrate amounts (50 g). On the day before a test, subjects were asked to restrict their intake of alcohol and caffeine-containing drinks and to avoid intense physical activity. The order of test foods was randomized, and all the foods were tested after a 12-h overnight fast. Blood glucose concentrations were measured in the capillary whole blood obtained by finger prick (Accu-Chek^® Fastclix Lancing Device, Castle Hill, NSW, Australia) in the fasted state and at 15, 30, 45, 60, 90, and 120 min after the start of the meal. Blood glucose was measured using a calibrated Accu-Chek^® Performa glucometer (Accu-Chek Performa, Roche Diagnostic, Castle Hill, NSW, Australia).

Kouamé C.A., Kouassi N.K., Abodo J.R., Pereko K.K., Casiraghi M.C., N’dri D.Y, & Amani G.N. (2017). Glycemic Responses, Glycemic Index, and Glycemic Load Values of Some Street Foods Prepared from Plantain (Musa spp., AAB Genome) in Côte d’Ivoire. Foods, 6(9), 83.

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Blood Glucose Measurement Protocol

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Blood glucose levels were measured using an Accu-Chek Performa glucose meter (Roche, Basel, Switzerland) and Accu-Chek Performa testing strips (06454011; Roche, Basel, Switzerland).

La Rose A.M., Bazioti V., Hoogerland J.A., Svendsen A.F., Groenen A.G., van Faassen M., Rutten M.G., Kloosterhuis N.J., Dethmers-Ausema B., Nijland J.H., Mithieux G., Rajas F., Kuipers F., Lukens M.V., Soehnlein O., Oosterveer M.H, & Westerterp M. (2021). Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia. Molecular Metabolism, 53, 101265.

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Glucose Reabsorption and TEER Measurement

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Glucose reabsorption was measured using a glucose meter (Accu-Chek Performa, Roche Diagnostics, Kwai Chung, Hong Kong) and test strip. In the glucose reabsorption assay, the glucose level of the medium was measured using a commercial glucose meter (Accu-Chek Performa; Roche Diagnostics). After analysis, 20 μL of the medium was collected in the medium chamber of the chip and dropped on a glucose meter test strip to measure the glucose concentration.
Trans-epithelial electrical resistance (TEER) is a well-known quantitative technique for measuring the integrity of tight junction dynamics in endothelial and epithelial fault cell culture models. Prior to evaluating the transport properties of drugs and chemicals, TEER levels are used as a reliable indicator of cell barrier integrity. TEER measurements have the advantage of being able to be performed in real time without cell damage. To measure TEER, an insert containing cells cultured on a chip for 14 days was transferred to a 6-well plate and then filled with badges. As TEER values can be affected by external factors such as temperature, paper discharge form, and cell algebra, the measurement was conducted with maximum error [22 (link)].

Lee J.B., Kim H., Kim S, & Sung G.Y. (2022). Fabrication and Evaluation of Tubule-on-a-Chip with RPTEC/HUVEC Co-Culture Using Injection-Molded Polycarbonate Chips. Micromachines, 13(11), 1932.

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Glucose and Insulin Tolerance Tests

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After overnight fasting, the tail blood glucose concentration (mM) was recorded (designated time = 0 min), mice were injected intraperitoneally with glucose at a dose of 2 g/kg of body weight, and blood glucose levels were monitored using a handheld glucometer (ACCU-CHEK Performa, Roche) at 15, 30, 60, and 120 min.
For ITTs, all mice were starved for 12 h, injected with a bolus of insulin (0.3 unit/kg of body weight), and their blood glucose levels were monitored using a handheld glucometer (ACCU-CHEK Performa, Roche) at 15, 30, 60, and 120 min.

Zhang R., Kan J.B., Lu S., Tong P., Yang J., Xi L., Liang X., Su D., Li D, & Liu Y. (2019). S100A16-induced adipogenesis is associated with up-regulation of 11 β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). Bioscience Reports, 39(9), BSR20182042.

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Glycerol Aerosol Exposure Impacts on Glucose and Glycerol Metabolism

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To assess the impact of glycerol aerosol exposure on glucose and glycerol circulating levels, glucose and glycerol tolerance tests were conducted following 6 and 7 weeks of exposure, respectively. Mice were fasted for 12 h (from 20:00 to 08:00) prior to experiments. For glycerol tolerance test, all mice were subjected to a 2 g/kg glycerol intraperitoneal injection. Glucose was measured using a glucometer (Accu‐Chek Performa; Roche) and blood was collected from the tail vein before and at 30, 60, 90, and 120‐min post‐injection. For glucose tolerance test, all mice were injected with 1 g/kg of d‐glucose. Glucose was measured using whole blood and a glucometer (Accu‐Chek Performa; Roche) before and at 15, 30, 45, 60, 90, and 120‐min post‐injection. Serum was isolated from blood and treated with Carrez Clarification Reagent Kit (ab202373; Abcam). Free glycerol was measured according to the manufacturer's instructions (ab65337; Abcam).

Lechasseur A., Mouchiroud M., Tremblay F., Bouffard G., Milad N., Pineault M., Maranda‐Robitaille M., Routhier J., Beaulieu M., Aubin S., Laplante M, & Morissette M.C. (2022). Glycerol contained in vaping liquids affects the liver and aspects of energy homeostasis in a sex‐dependent manner. Physiological Reports, 10(2), e15146.

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