Infrared spectra were collected on a Thermo-Nicolet Avatar 360 FT-IR spectrophotometer equipped with a single reflection Smart Orbit diamond ATR aperture in the range of 4000 – 400 cm-1. Fluorescence spectra were collected on a Varian Cary Eclipse Fluorescence spectrophotometer. Diffuse reflectance UV-Vis spectroscopy measurements were collected on powder samples using a Cary 5000 spectrophotometer. Bandgap values are estimated using Kubelka-Munk theory.32 , 33 Raman data was collected on a Thermo Nicolet 870 instrument that was coupled to an FT-Raman module. X-ray photoelectron spectroscopy was conducted on a PHI 5000 Versaprobe II Scanning ESCA microprobe using a monochromatic Al Kα X-ray source (1486.6 eV). The base vacuum in the chamber was better than 1.5 × 10-10 torr. The samples used in this study were probed by an X-ray source with a power of 100W and a beam diameter of 150μm. Survey scans were collected on several different areas to study the relative composition of the sample. High resolutions scans were performed on each elemental region to improve the signal-to-noise ratio. Sample charging effects were minimized using a low energy electron gun and Ar+ ions. The binding energy scale was referenced to the C1s peak (284.8 eV) to accommodate peak shifts as a consequence of sample charging effects.
Cary 5000 spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States, Canada, Australia, France
The Cary 5000 spectrophotometer is a versatile instrument designed for advanced spectroscopic analysis. It measures the absorption, transmission, or reflectance of light through a sample across a wide range of wavelengths, from the ultraviolet to the near-infrared spectrum.
Automatically generated - may contain errors
Lab products found in correlation
Fluorolog 3 spectrofluorometer, by Horiba (4 mentions)
Fluoromax 4, by Horiba (3 mentions)
8453 spectrophotometer, by Agilent Technologies (3 mentions)
241 polarimeter, by PerkinElmer (3 mentions)
Invia raman spectrometer, by Renishaw (3 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (2 mentions)
Dra 2500 integrating sphere, by Agilent Technologies (2 mentions)
Jem 1010 microscope, by JEOL (2 mentions)
Jsm 6700f feg, by JEOL (2 mentions)
D8 advance, by Bruker (2 mentions)
Apreo s lovac, by Thermo Fisher Scientific (2 mentions)
Jem 2100, by JEOL (2 mentions)
Phi 5500, by PerkinElmer (2 mentions)
S 4800 scanning electron microscope, by Hitachi (2 mentions)
Tecnai 12 transmission electron microscope, by Philips (2 mentions)
S 4800 2 field emission scanning electron microscope, by Hitachi (2 mentions)
Lsm 700 3d laser scanning microscope, by Zeiss (2 mentions)
Cary 610 670 micro infrared spectrometer, by Agilent Technologies (2 mentions)
Fluorescence spectrofluorometer, by Horiba (2 mentions)
Tecnai f30 electron microscope, by Thermo Fisher Scientific (1 mentions)
116 protocols using cary 5000 spectrophotometer
1
Comprehensive Characterization of Material Properties
2
Cationic Oxidation of Cycloparaphenylenes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Et3O+SbCl6– was used to generate the cations and dications in 10–4 M solution of [n]CPPs in CH2Cl2. Oxidations were monitored by UV-Vis-NIR absorption using a Cary 5000 spectrophotometer.
3
Nanorod-Virus Binding Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PEGylated nanorods or nanorods loaded with annexinV were centrifuged at 5000 rpm for 10 min. The supernatant was discarded and 17 µL of the pellet was added to 50 µL of MLV/luc, followed by overnight incubation at 4 °C. Then, 500 µL of IgG antibody (anti-p24gag monoclonal antibody; Clone 183-H12-5C; NIH AIDS Research and Reference Reagent Program, contributed by Dr. Bruce Chesebro) in RPMI medium was added to the mixture and the mixture was diluted by adding 1x Tris (pH 7) or PBS (pH 7.4) buffer to final volume of 1 ml. The nanorod to virus ratio was more than 10000:1. The concentration of the nanorods was quantified using a UV-vis spectrometer (Cary 5000 spectrophotometer).
4
Tuning Nanoparticle Plasmon Shift
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RIS measurements where performed in water-glycerol mixtures of varying volume ratios to tune the refractive index of the media surrounding the nanoparticles. As-prepared hollow nanoparticles were concentrated 20 times by centrifugation (i.e., 5 mL of NP solution was dispersed in 250 μL of DI water). Then 10 μL of nanoparticle dispersion were added to 2.0 mL of the water–glycerol solutions. Solutions containing 0%, 2.5%, 5%, 7.5%, 10%, 15%, 20%, 30%, 40% and 50% v/v of glycerol yielded refractive indices ranging from 1.333 to 1.403. Extinction spectra of the resulting nanoparticle solutions were measured on a Cary 5000 spectrophotometer. The plasmon shift in nm was plotted as a function of the refractive index and the RIS was determined by linear fitting (Δλsp/Δnm).
5
Kinetic Analysis of Laccase-Catalyzed Oxidation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enzyme assays were performed in triplicate in 1 mL at 30 °C using a Cary 5000 spectrophotometer fitted with a thermostatted cuvette holder. The steady-state kinetics parameters for ABTS oxidation were obtained by monitoring the change in absorbance at 414 nm (ε 36.6 mM−1 cm−1) in reaction mixtures containing 0.01–10 mM ABTS, 0.1 μM sLac, and 50 mM sodium acetate at pH 5. Those for syringol were obtained by monitoring the change in absorbance at 468 nm (ε = 37.5 mM−1 cm−1) in reaction mixtures containing 0.05–10 mM syringol, 0.1 μM sLac, and 50 mM Tris-HCL (pH 8).
6
Absorption and Luminescence Spectroscopy of Mn(II) Crystals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The
absorption spectra of monocrystals were measured on a Cary 5000
spectrophotometer. The temperature measurements of the crystals were
performed as follows: the sample was placed into a small teflon holder,
which was fixed in a 1 cm quartz cuvette filled with paraffin oil.
The temperature of the samples was regulated using a temperature controller
TC 125.
The experimental oscillator strengths (Pexp) were determined by usingeq 1 where c is the concentration
of the Mn(II) ion in M, d is the length of the optical
way in cm, and A(ν̅) is the absorbance
as a function of the wavenumber in cm–1. The luminescence
decay curves of crystals were detected on an Edinburgh Instruments
FLS 920 spectrometer with the monitored emission at 530 nm.
absorption spectra of monocrystals were measured on a Cary 5000
spectrophotometer. The temperature measurements of the crystals were
performed as follows: the sample was placed into a small teflon holder,
which was fixed in a 1 cm quartz cuvette filled with paraffin oil.
The temperature of the samples was regulated using a temperature controller
TC 125.
The experimental oscillator strengths (Pexp) were determined by using
of the Mn(II) ion in M, d is the length of the optical
way in cm, and A(ν̅) is the absorbance
as a function of the wavenumber in cm–1. The luminescence
decay curves of crystals were detected on an Edinburgh Instruments
FLS 920 spectrometer with the monitored emission at 530 nm.
7
Characterization of Nanoparticle Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy (TEM) images were acquired using a JEOL JEM-2010 machine operated at 200 kV acceleration voltage. A FEI Tecnai F30 electron microscope was used for high-resolution imaging (HRTEM). All of the absorption spectra were recorded using a Cary 5000 spectrophotometer. Solution-phase photoluminescence was measured using Horiba Jobin Yvon Fluorolog spectrometer.
8
Dispersion and Characterization of MWNTs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A dispersal solution (10 mL) containing 0.2 mg/mL MWNTs in an aqueous solution of 5 v/v % Triton X-100 was sonicated in an ice-bath with a microprobe (QSonica, Q700) for 20 min with 2 s pulse on and 3 s pulse off time at 50% amplitude and a power of 4–5 W. Aqueous dispersions were centrifuged at 14,000 rpm for 10 min to remove MWNTs that were not dispersed. The black-colored supernatant was pipetted into a clean falcon tube. Concentration of MWNT dispersions were determined by absorbance spectroscopy (Cary 5000 spectrophotometer) using the specific extinction coefficient for MWNTs at 500 nm (ɛ500 = 46 mL mg−1 cm−1) [26 (link)].
9
Physicochemical Characterization of Lauromacrogol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The study of physicochemical properties of lauromacrogol was performed using optical spectroscopy techniques, including UV-Vis-NIR absorption, infrared and micro-Raman spectroscopy. The absorption spectrum was determined using a Cary 5000 spectrophotometer (Santa Clara, CA, USA) with the 175–3300 nm range on the quartz plate. The spectrum obtained was in the 300–2500 nm range with a resolution of 0.5 nm. A Nicolet iS10 FT-IR spectrometer (Waltham, MA, USA) equipped with an automated beam splitter exchange system (iS50 ABX containing a DLaTGSKBr detector) with a HeNe laser as an IR radiation source and built-in all-reflective diamond ATR module (iS50 ATR), Thermo Scientific Polaris™, was used for the infrared spectrum measurements. The spectra detected were in the range of 400–4000 cm−1 with a frequency resolution of 4 cm−1. A potassium bromide (KBr) plate was used for the measurement. The Micro-Raman spectrum was determined with a Renishaw InVia micro-Raman system (Wotton-under-Edge, Gloucestershire, UK) equipped with a Leica DM 2500 M microscope and a CCD camera for detection (3500–200 cm−1). The spectrum recorded was in the range 50–3300 cm−1 when excited at 514 nm.
10
Nanomaterial Characterization Techniques
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy (TEM) images and selected area electron diffraction (SAED) patterns were obtained on a JEOL-1011 (100 kV). Samples for the TEM analysis were prepared by drop-casting 10 μL dispersion of nanocrystals diluted in toluene onto carbon-coated copper or titanium TEM grids followed by solvent evaporation. High resolution (HR) TEM images were obtained with a Philips CM 300 UT microscope operated at 200 kV. X-ray diffraction (XRD) measurements were performed with a Philips X'Pert System with Bragg–Brentano geometry, equipped with a copper anode (Kα X-ray wavelength of 0.154 nm). Samples were prepared by drop-casting the colloidal nanocrystal solution onto silicon wafer substrates (<911> or <711> cut) with subsequent solvent evaporation. Atomic force microscopy (AFM) measurements were performed on a JPK Instruments system (JPK Nano Wizard 3) in intermittent contact mode. UV-VIS-NIR absorption spectra were obtained with a Cary 5000 spectrophotometer equipped with an integration sphere.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!