Mibefradil (Sigma Aldrich, Dorset, UK) and verapamil (Sigma Aldrich) were made from 10 mM stock solutions in distilled water and stored at 4 °C, and every two months, fresh stock solutions were prepared. TH1177 was a kind gift from Dr Lloyd Gray, University of Virginia, Charlottesville, Va., USA. TH1177 was prepared from a 10 mM stock solution in 100% ethanol and stored at -20 °C.
Mibefradil
Manufactured by Merck Group
Sourced in United States, United Kingdom, France, Japan
Mibefradil is a laboratory equipment product from Merck Group. It is a calcium channel blocker used for research and scientific applications. The core function of Mibefradil is to modulate calcium ion channels in cellular environments.
Automatically generated - may contain errors
Lab products found in correlation
Nifedipine, by Merck Group (7 mentions)
Verapamil, by Merck Group (5 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Nnc 55 0396, by Merck Group (2 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (2 mentions)
Fluo 4 am, by Thermo Fisher Scientific (2 mentions)
Ruthenium red, by Merck Group (2 mentions)
Capsaicin, by Merck Group (2 mentions)
Probenecid, by Merck Group (2 mentions)
Efonidipine, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Fujifilm (2 mentions)
Mouse igg1 isotype control antibody, by BioLegend (1 mentions)
Glutamine, by Sartorius (1 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Anti human cd3, by Thermo Fisher Scientific (1 mentions)
Hepes, by Sartorius (1 mentions)
Cd45 proteins, by BioLegend (1 mentions)
Rpmi 1640, by Sartorius (1 mentions)
Ionomycin, by Alomone (1 mentions)
33 protocols using mibefradil
1
Small Molecule Compound Preparation
2
Mibefradil Infusion for Lateral Hippocampus
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Within 6 h after SE onset, the rats were anesthetized and fixed into the stereotaxic apparatus. Each mini-osmotic pump (ALZET® micro-osmotic pump, 1007D; Durect Corporation, Cupertino, CA, USA) was attached to a cannula and placed in a subcutaneous pocket. Subsequently, the cannula was implanted into the lateral hippocampus (positions: −4.0 mm posterior to bregma, 3.0 mm bilateral to the midline, and 3.1 mm under the skull surface) with infusion of Mibefradil (1.5 mg/ml in 0.1 M PBS; Sigma-Aldrich) for seven consecutive days (19 (link),20 (link)). The cannula was secured with dental acrylic cement.
3
Anti-amyloid beta aggregates therapy
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The monoclonal antibody 3A1 was generated by Dr. Brian O’Nuallain against dityrosine cross-linked Aß1–40 with no detectible binding to APP and an ∼700 fold preference for soluble cross-linked Aß aggregates over Aß monomers in Capture/Sandwich ELISA (Frost et al., 2017 ), and mouse IgG1 isotype control antibody (Biolegend, United Kingdom) were administered in 5 i.c.v. injections (20 μg in 5 μl per injection) over 3 days with the last injection 2 h prior to HFS. We chose this regimen because we found a similar protocol was effective for other anti-Aß strategies in this model. A 20 μg dose of 3A1 was selected because a 10 μg treatment regimen did not reverse the LTP deficit in pilot experiments (n = 2, data not shown). Mibefradil (50 nmol in 5 μl i.c.v., Sigma) and (R)-3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 7 mg/kg, i.p., Ascent Scientific) were dissolved in distilled water and administered 30 min and 2 h prior to HFS, respectively. The doses were chosen based on their ability to inhibit LTP with different HFS protocols in WT rats (Doyle et al., 1996 (link); Ryan et al., 2010 (link)).
4
Cell Culture and Protein Reagents
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Complete Medium (medium): RPMI-1640, DMEM medium, heat-inactivated fetal calf serum (FCS), penicillin, streptomycin, glutamine, sodium pyruvate, and HEPES obtained from Biological Industries (Kibbutz Beit Haemek, Israel). Mibefradil from Sigma-Aldrich (St. Louis, MO, USA). CD45 proteins were purchased from BioLegend (San Diego, CA, USA). Anti-human CD3 from eBioscience Inc. (ThermoFisher Scientific, Waltham, MA, USA).
5
Calcium Signaling in Sperm Capacitation
6
Calcium Channel Blocker Evaluation
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All animal protocols were approved by the Institutional Animal Ethical and Welfare of West China Hospital of Sichuan University
Two types of CCBs were evaluated in the present study. Both Nifedipine (L-CCB) and Mibefradil (T&L-CCB) were obtained from Sigma-Aldrich (St. Louis, MO).
Two types of CCBs were evaluated in the present study. Both Nifedipine (L-CCB) and Mibefradil (T&L-CCB) were obtained from Sigma-Aldrich (St. Louis, MO).
7
Sensitivity to Trastuzumab in Cancer Cells
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Cells were treated with mibefradil (0.01–1 µM; Sigma Aldrich) [33 (link)] or ML218 (0.1–10 µM; Sigma Aldrich) [34 (link)] either alone or in combination with trastuzumab (10 µg/mL), 24 h after seeding (2000 cells/well in a 96 well plate). All treatments and media changes were repeated every 2 days in antibiotic-free media. Sensitivity to trastuzumab was assessed using a MTS assay 192 h after treatment start.
8
Pharmacological Evaluation of Ion Channel Modulators
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Drugs were made up in dimethyl sulphoxide (DMSO), ethanol, or water depending on solubility. Stock solutions were added to the drug delivery reservoirs containing Hanks solution to make up the final concentrations. Drugs used were as follows: KB‐R7943, Tocris (Bristol, UK); Mibefradil, Sigma (Wicklow, Ireland); Nifedipine, Bayer (Leverkusen, Germany); SEA0400 was synthesized by Taisyo Pharmaceutical Co., Ltd., Saitama, Japan. Mibefradil was water soluble, while SEA0400 and KB‐R7943 were dissolved in DMSO and diluted with Hanks solution to give a final DMSO concentration of 0.1% and 0.05%, respectively. Nifedipine was first dissolved in ethanol and diluted with Hanks solution to give a final ethanol concentration of 0.1%. Control experiments showed that these concentrations of vehicle had no significant effects on the responses measured in this study.
The cell under study was continuously superfused with Hanks solution by means of a close delivery system consisting of a pipette (tip diameter 200 μm) placed approximately 300 μm away. This could be switched, with a dead space time of around 5 sec, to a solution containing a drug. All experiments were carried out at 35–37°C.
The cell under study was continuously superfused with Hanks solution by means of a close delivery system consisting of a pipette (tip diameter 200 μm) placed approximately 300 μm away. This could be switched, with a dead space time of around 5 sec, to a solution containing a drug. All experiments were carried out at 35–37°C.
9
Intravitreal Injection Procedure for Ophthalmic Research
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Intravitreal injection was performed according to previously described method (16 (link),24 (link)). When the pupil of the anesthetized eye was dilated with tropicamide drops, clustered EphB2-Fc (2 µl,), or Mibefradil (no. M5441, 3 mM; Sigma-Aldrich; Merck KGaA) dispersed in 2 µl of 0.9% saline, was injected into the vitreous space through a postlimbus spot using stereoscopic microscope (Carl Zeiss). A 30-gauge needle was then inserted 2 mm behind the temporal limbus and directed toward the optic nerve. Eyes of vehicle or negative control (Ctr) group were injected with saline or clustered human Ig-Fc (2 µg; R&D Systems, Inc.), respectively.
10
Serotonin Signaling Pathway Analysis
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