Cg prkdcscid il2rgtm1wjl szj

NOD. Cg-Prkdc^scid IL2rg^tm1Wjl/SzJ, most often known by their branded name, NOD scid gamma (abbreviated as NSG) mice (3–4 weeks old) were purchased from The Jackson Laboratory (Bar Harbor, ME). All mice were housed in a pathogen free facility in microisolator cages following the established protocols of the Ethics Committee of the Science Faculty and National and International Legislation for Live Animal Experimentation (Colombia Republic, Resolution 08430, 1993; National Academy of Sciences, 2010) (Act 006-2014, approved on June 20, 2014). Mice were housed in polyethylene cages with sterile food and water ad libitum, controlled temperature, and a 12 h light/dark cycle. Before treatment, the mice were familiarized for 1 week under standard conditions. The ethics committee of the science faculty approved the format for animals use on June 24, 2014.
1 × 10⁶ MDA-MB-468 cells were orthotopically transplanted on NSG mice. After 8 days, the mice (4 mice per group) were treated intraperitoneally with P2Et (18.7 mg/kg) or PBS twice per week, until endpoint. Tumours were measured with Vernier callipers twice a week, and the experiment was finished when mice showed end-point symptoms, humanely defined in the protocol. The tumour volume was calculated using the following formula: tumour volume (mm³) = [(Width)² × Length]/2.

All pancreatic tumour tissue specimens were obtained during clinically indicated collection procedures with informed consent and authorised through institutional review board (IRB)-approved bio-specimen protocol number 14-091 at Memorial Sloan Kettering Cancer Center. Samples were de-identified before receipt for use in these approved studies. Samples were either used within 30 min to 1 h of collection for ex vivo studies or were minced, mixed with matrigel (50:50) and implanted subcutaneously in the flank of female NSG mice (Jackson Laboratory, Bar Harbour, NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ, female, 20–25 g, 8 weeks old, IMSR Cat# JAX:005557, RRID: IMSR_JAX:005557) to generate PDX models, as previously described^{51 (link)}. All mice were cared for in accordance with guidelines approved by the Memorial Sloan Kettering Cancer Center Institutional Animal Care and Use Committee under an approved IACUC protocol (04-03-009).