Pluronic pe 10500

Isomeric purity of (Z)-4-undecenal (Z4-11Al) was 98.6%, according to gas chromatography coupled to mass spectrometry (6890 GC and 5975 MS, Agilent Technologies, Santa Clara, CA, USA). Isomeric purity of (Z)-4-nonenal (Z4-9Al) and (Z)-6-undecenal (Z6-11Al) were 97.4 and 96%, repectively. Chemical purity of these synthetic aldehydes was >99.9%. Ethanol (redistilled; Merck, Darmstadt, Germany) was used as solvent.
For the OR screening assays, the following chemicals were used: Dulbecco’s MEM medium (#F0435), FBS superior (#S0615), L-glutamine (#K0282), penicillin (10000 U/ml)/streptomycin (10000 μg/mL) (#A2212), trypsin/EDTA solution (#L2143) (Biochrom, Berlin, Germany), CaCl2∗2H2O (#22322.295), D-glucose (#101174Y), dimethyl sulfoxide (DMSO) (#83673.230), HEPES (#441476L), potassium chloride (#26764.230), and sodium hydroxide (#28244.295) (VWR Chemicals BDH Prolabo, Leuven, Belgium), sodium chloride (#1064041000, Merck, Darmstadt, Germany), ViaFect™ Transfection Reagent (#E4981, Promega, Walldorf, Germany), D-luciferin (beetle) monosodium salt (#E464X, Promega, Walldorf, Germany), Pluronic® PE 10500 (#500053867, BASF, Ludwigshafen, Germany), (R)-(−)-carvone (#W224908, Sigma-Aldrich, Steinheim, Germany).

Concentration–response assays
were measured 42 h post-transfection as described previously.^{102 (link)} In short, the supernatant was removed, and
cells were loaded with a physiological salt buffer (pH 7.5) containing
140 mmol/L NaCl, 10 mmol/L HEPES, 5 mmol/L KCl, 1 mmol/L CaCl2, 10
mmol/L glucose, and 2% of beetle luciferin sodium salt (Promega, Madison,
USA). For luminescence measurements, the GloMax Discover microplate
reader (Promega, Madison, USA) was used. After incubation for 50 min
in the dark, the basal luminescence signal of each well was recorded
thrice. Then the odorant, serially diluted in the physiological salt
buffer with added Pluronic PE-10500 (BASF, Ludwigshafen, Germany),
was applied to the cells, and luminescence was measured thrice after
10 min of incubation time. The final Pluronic PE-10500 concentration
on the cells was 0.05%.