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10 protocols using acunav

1

Ablation Procedures Under General Anesthesia

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All ablation procedures were performed under general anesthesia. Bilateral femoral access was obtained with ultrasound guidance using the modified Seldinger technique. Intravenous heparin was administered as boluses and continuous infusion to maintain a target ACT of >300 s. In both groups, esophageal temperature monitoring was performed using a commercial temperature probe (SensiTherm, Abbott). Ablation lesion delivery was halted if the esophageal temperature exceeded 39°C.
Under ICE catheter (AcuNav, Biosense Webster Inc) guidance, a transseptal puncture was made using the modified Brockenbrough technique. An 8.5 F SL1 sheath (Abbott Laboratories) was advanced into the left atrium. For cases undergoing RF‐WACA, a second transeptal puncture was performed in a similar fashion.
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2

Cryoballoon Ablation for Pulmonary Vein Isolation

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All 100 patients underwent cryoballoon ablation (CB) (Artic Front Advance™, Medtronic, Minneapolis, MN). Standard procedures were used, including use of intracardiac echocardiography (AcuNav, Biosense Webster), fluoroscopy, and electroanatomic mapping (NavX, Abbott, and CARTO, Biosense Webster). Briefly, for CB, a minimum of two cryoablations were delivered per pulmonary vein (PV) with ablation lasting 3–4 minutes to a nadir temperature < 55°C. If PV isolation (PVI) was not achieved with CB, then focal ablation was performed using either Cryocatheter (Cryocath, Medtronic) or radiofrequency catheter to achieve PVI. Entry block was confirmed by pacing from the distal poles of the coronary sinus catheter for the left sided PVs and elimination of right sided PVs. Exit block in all PVs was evaluated by pacing around the circular mapping catheter placed sequentially in each PV.
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3

Comprehensive Cardiac Ablation Procedure

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The right femoral vein and left median cubital vein of the forearm were used to gain vascular access. A 6 Fr 20-pole steerable catheter (BeeAT, Japan Lifeline, Tokyo, Japan), capable of recording from three sites simultaneously, was inserted through the left median cubital vein and placed into the coronary sinus, the right atrium, and the superior vena cava for pacing, recording, and internal cardioversion (23 (link)). An intracardiac echocardiography (ICE) probe (AcuNav or SoundStar, Biosense Webster, Diamond Bar, USA) was placed on the right atrial septum via the femoral vein. Transseptal access to the left atrium was established using an RF needle (Baylis Medical, Montreal, Canada) and an 8Fr or 8.5Fr long sheath (SL0, Abbott, MN, USA) under fluoroscopic and ICE guidance. During the procedure, a state of systemic anticoagulation was ensured through repetitive intravenous heparin administration to maintain an activated clotting time of 300-350 seconds. A 7Fr esophageal catheter (Esophastar, Japan Lifeline) was inserted nasally and advanced into the esophagus, posterior to the left atrium, under fluoroscopic guidance. Esophageal temperature was continuously monitored during the ablation and the application was discontinued if the temperature reached 39℃ (RF-PVI) or 15℃ (CB-PVI).
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Comprehensive Cardiac Function Assessment in Rats

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Baseline cardiac function was assessed prior to the index procedure. Rats were weighed, and drug dosages were calculated per weight. Sedation was performed by placing the rat in an induction chamber with 5% isoflurane in 100% oxygen for few minutes, and confirming sedation with a toe pinch. Maintaining anesthesia on a nose cone thereafter, transthoracic echocardiography was performed (Vevo 2100, FUJIFILM Visual Sonics, Inc). The rat was then intubated with a 16G angiocath, moved onto a heated surgical platform and placed right lateral decubitus. Mechanical ventilation (Inspira AV; Harvard Apparatus, Holliston, MA) was started with 2% isoflurane in 1 lpm of oxygen at 66 breaths/minute, with a tidal volume of 1ml/100g body weight. Six-lead electrocardiogram was recorded throughout the procedure (Mouse monitor, Indus Instruments, TX), body temperature was monitored, and heating was adjusted as needed. An 8Fr intracardiac echo probe (AcuNav, Biosense Webster) was inserted into the esophagus for transesophageal cardiac imaging of the left sided chambers and mitral valve hemodynamics.
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5

PV Mapping and Ablation Procedure Protocol

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All patients underwent an electrophysiological study in the fasting state under conscious sedation. A 20‐pole catheter was inserted through the right jugular vein (BeeAT® Japan Lifeline). The proximal portion of the catheter was positioned along the superior vena cava and crista terminalis, and the distal portion was positioned in the coronary sinus (CS) for pacing and internal cardioversion.
Following a trans‐septal puncture under guidance with an intracardiac echocardiography catheter (5.5–10 MHz, 8Fr, AcuNav™, Biosense Webster), two or three long sheaths (SL1®, AF Division, St. Jude Medical) were introduced into the LA via the same trans‐septal puncture site. After a left atriography was performed, 20‐pole circular mapping catheters (1–5–1 mm interelectrode spacing, 20 mm in diameter, and/or 1–3.5–1 mm interelectrode spacing, 15 mm in diameter) and a 3.5 mm open‐irrigated‐tip ablation catheter (Navistar® Thermocool®, Biosense Webster or Thermocool Smarttouch®, Biosense Webster, or TactiCath™, Abbott) were positioned in the PVs for PV mapping (Figure 1A). In four patients, PV mapping was also performed with a 64‐pole basket catheter (Constellation®, Boston Scientific) (Figure 1B). The electrophysiological studies were performed under support of an electroanatomical mapping system with the CARTO® system (Biosense Webster) or Ensite Velocity™ system (St. Jude Medical).
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6

Sonothrombolysis using endosonography probe

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A 10 F endosonography probe (AcuNav, Biosense Webster®) was used in combination with the ACUSON SEQUOIA™ 512 ULTRASOUND SYSTEMS Siemens. The probe was placed via the drainage catheter in the clot core for sonothrombolysis. The endosonography probe has a cone shaped field of view (22° x 90°) absorbing ultrasound laterally. Ultrasound treatment was performed for 1h, while after 30 min it was turned 180° in order to reach a maximum field of the clot (Fig 1). Different ultrasound frequencies were investigated (10 MHz, 8.5 MHz, 7.5 MHz, 5.5 MHz). Furthermore the lowest possible mechanical index (MI) was adjusted on our device (10 MHz: MI 0.55; 8.5 MHz: MI 1; 7.5 MHz: MI 1.1; 5.5 MHz: MI 1).
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7

Percutaneous Cardiac Intervention Protocol

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Animals were anesthetized with atropine, butorphanol, ketamine, and xylazine, and maintained on isoflurane and mechanical ventilation. Percutaneous arterial and venous access was obtained. If patent foramen ovale was not present, standard Mullins technique transseptal puncture was performed. Atrial septal communications were enlarged by inflation of large (18–20 mm diameter) balloon angioplasty catheters. Experiments were guided by X-ray fluoroscopy (Siemens Medical, Erlangen, Germany) and intracardiac echocardiography (AcuNav, Biosense-Webster, Diamond Bar, CA).
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8

Cryoballoon Ablation for Pulmonary Vein Isolation

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A single transseptal puncture was performed under fluoroscopic and intracardiac echocardiographic guidance (AcuNav, Biosense Webster). Thereafter, heparin was administered intravenously to maintain an activated clotting time over 300 seconds. The second‐generation 28 mm CB catheter was introduced into the LA through a steerable sheath (FlexCath Advance, Medtronic). A mapping catheter (Achieve, Medtronic) was advanced within the CB to the PV. The CB was inflated and advanced to the ostium of each PV. Cryothermal energy was applied for 180 seconds with the CB. The order of CB application was as follows; left superior PV (LSPV), left inferior PV (LIPV), right inferior PV (RIPV), and right superior PV (RSPV). If necessary, PV isolation was completed with additional CB applications or an irrigated radiofrequency catheter.
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9

Fluoroscopy-free Cardiac Catheterization

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Most procedures were performed in conscious sedation, except in paediatric patients, where general anaesthesia was used. Right femoral vein punctures were performed to access the heart. All procedures were performed with the three dimensional (3D) electro-anatomic mapping (EAM) system (NavX™, Abbott, Abbott Park, Illinois, USA) and intra-cardiac echocardiography (AcuNav™, Biosense Webster, Irvine, California, USA) without the use of fluoroscopy.
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10

Intracardiac Echocardiography Protocol

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Intracardiac echocardiography (ICE) was performed from the right atrium and right ventricle, using the AcuNav (Biosense Webster, Diamond Bar, CA) or the ViewFlex Xtra (St. Jude Medical, St. Paul, MN) ultrasound catheter. If visualization was unsatisfactory, ICE from the aortic arch was also performed.
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