The catalase, peroxidase, and SOD enzyme activities in the spermathecal fluid were assayed using a Catalase Activity Colorimetric/Fluorometric Assay Kit (BioVision Inc.), Peroxidase Activity Colorimetric/Fluorometric Assay Kit (BioVision Inc.), and SOD Activity Assay Kit (BioVision Inc.) according to the manufacturer’s instructions.
Catalase activity colorimetric fluorometric assay kit
Manufactured by Abcam
Sourced in United States
The Catalase Activity Colorimetric/Fluorometric Assay Kit is a laboratory tool used to measure the activity of the enzyme catalase. Catalase is an important antioxidant enzyme that helps to break down hydrogen peroxide, a potentially harmful byproduct of cellular metabolism. The assay kit provides a quantitative method for determining catalase levels in various biological samples.
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Lab products found in correlation
Glutathione peroxidase activity colorimetric assay kit, by Abcam (3 mentions)
Peroxidase activity colorimetric fluorometric assay kit, by Abcam (2 mentions)
Superoxide dismutase sod activity colorimetric assay kit, by Abcam (2 mentions)
Superoxide dismutase activity assay kit, by Alexis Biochemicals (2 mentions)
Sod1 mouse elisa kit, by Abcam (2 mentions)
Superoxide dismutase sod activity assay kit, by Abcam (2 mentions)
Sod activity assay kit, by Abcam (1 mentions)
Spectrofluorometer, by Molecular Devices (1 mentions)
Glucose and sucrose colorimetric fluorometric assay kit, by Merck Group (1 mentions)
Activity colorimetric assay kit, by Abcam (1 mentions)
Mda colorimetric fluorometric assay kit, by Abcam (1 mentions)
Quick cell proliferation colorimetric assay kit, by Abcam (1 mentions)
Bisbenzimide h 33342 trihydrochloride, by Merck Group (1 mentions)
Glutathione colorimetric assay kit, by Abcam (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorodihydrofluorescein diacetate h2dcf da, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Trypan blue solution, by Merck Group (1 mentions)
Lipid peroxidation mda colorimetric fluorometric assay kit, by Abcam (1 mentions)
Glutathione assay kit, by Cayman Chemical (1 mentions)
17 protocols using catalase activity colorimetric fluorometric assay kit
1
Spermathecal Fluid Enzyme Assays
2
Colorimetric Assay for Astrocyte Catalase
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Catalase activity in astrocytes was detected using the catalase activity colorimetric/fluorometric assay kit (BioVision, USA). The cells (106) were incubated with 12 μl of fresh 1-mM H2O2 at 25 °C for 30 min, and 10 μl of stop solution was added. The samples were then incubated with 50 μl of Developer Mix (46 μl of assay buffer, 2 μl of OxiRed™ Probe and 2 μl HRP solution) at 25 °C for 10 min. Catalase activity was determined using a spectrofluorometer (Molecular Devices, USA) to measure the absorbance at 570 nm.
3
Antioxidant Enzyme Activity in Moth Orchids
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Leaf samples of the new fully expanded leaves (L1) of moth orchids with or without BL acclimation for 12 days (T0) and ML or HL exposure for two weeks were collected. A total of 100 mg of frozen leaf samples were homogenized. The SOD enzyme activity was determined using a Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (BioVision, Milpitas, CA, USA #K335-100) according to the instructions of the manufacturer. The SOD activity was calculated based on the percentage inhibition at 20 min at 37 °C. For the detection of the catalase enzyme activity, 100 mg of frozen leaf samples were homogenized and determined using a Catalase Activity Colorimetric/Fluorometric Assay Kit (BioVision, Milpitas, CA, USA #K773-100) according to the manufacturer’s instruction.
4
Evaluating Antioxidant Responses and Carbohydrate Dynamics in Vanilla Orchid Leaves
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Vanilla orchid newly expanded L3 leaf samples, with or without BL-acclimation for 12 days, were exposed to ML500 or HL1000 for two weeks and then collected and stored at −80 °C freezer. A total of 100 mg of frozen leaf samples were homogenized. SOD enzyme activity was determined using a Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (BioVision #K335-100), following the instruction of the manufacturer. SOD enzyme activity was calculated based on the percentage inhibition after 20 min of incubation at 37 °C. For detection of catalase (CAT) enzyme activity, 100 mg frozen leaf samples were homogenized and determined using a Catalase Activity Colorimetric/ Fluorometric Assay Kit (BioVision, #K773-100), as described previously [22 (link)].
Vanilla orchid L3 leaves, with or without BL-acclimation for 12 days, which had then been exposed to moderate light (ML500) or high light (HL1000) irradiation for two weeks, were collected. A total of 100 mg of leaf samples were homogenized, 500 μL of 1 × PBS buffer was added, and then the mixture was incubated for 30 min at 37 °C. Then, glucose and sucrose concentrations were determined using a Glucose and Sucrose Colorimetric/Fluorometric Assay Kit (Sigma #MAK013), following the instructions provided by the manufacturer.
Vanilla orchid L3 leaves, with or without BL-acclimation for 12 days, which had then been exposed to moderate light (ML500) or high light (HL1000) irradiation for two weeks, were collected. A total of 100 mg of leaf samples were homogenized, 500 μL of 1 × PBS buffer was added, and then the mixture was incubated for 30 min at 37 °C. Then, glucose and sucrose concentrations were determined using a Glucose and Sucrose Colorimetric/Fluorometric Assay Kit (Sigma #MAK013), following the instructions provided by the manufacturer.
5
Antioxidant Enzyme Activity Assays
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Mn-SOD and CuZn-SOD activity was determined using a Superoxide Dismutase Activity Assay Kit (Alexis Biochemicals). The catalase activity was measured using a Catalase Activity Colorimetric/Fluorometric Assay Kit (BioVision, Inc.), and GSH-Px was determined using the Glutathione Peroxidase Assay Kits (Biocompare, Inc.) following the manufacturers’ guidelines.
6
Antioxidant Enzyme Assays in Mice
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Dorsal skin tissues were collected from mice, and the activities of SOD, CAT, and GPx were measured using respective assay kits purchased from BioVision, Inc. (Milpitas, CA, USA); superoxide dismutase 1 (SOD1) (Mouse) ELISA Kit, Catalase Activity Colorimetric/Fluorometric Assay Kit, and Glutathione Peroxidase Activity Colorimetric Assay Kit, according to methods described previously [24 (link)].
7
Measuring Antioxidant Enzyme Activities
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The Superoxide Dismutase Activity Assay Kit (Alexis Biochemicals) and Catalase Activity Colorimetric/Fluorometric Assay Kit (BioVision Inc.) were used for the detection of Mn-SOD and catalase activity, following the manufacturer's guidelines. Enzyme activities were displayed as fold change compared to the control.
8
Oxidative Stress Biomarkers in Tissue
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After the routine skin tissue homogenization and centrifuge processes, the BioVision brand Glutathione Peroxidase Activity Colorimetric Assay Kit was used to determine the GSH. The reading was performed using a BMG LABTECH 96 Microplate Reader at 340 nm.
To determine the MDA in the tissue, the BioVision brand Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit was used, and the measurements were used with the Colorimetric method. The reading was performed using a BMG LABTECH 96 Microplate Reader at 532 nm. A BioVision brand Catalase Activity Colorimetric/Fluorometric Assay Kit was used with the Colorimetric method to determine the catalase in the tissue. The reading was performed with a BMG LABTECH 96 Microplate Reader at 570 nm. We used a Beckman Coulter AU640 brand auto-analyzer applique and a Randox Ransod brand commercial kit to determine the SOD activity in the tissue.
To determine the MDA in the tissue, the BioVision brand Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit was used, and the measurements were used with the Colorimetric method. The reading was performed using a BMG LABTECH 96 Microplate Reader at 532 nm. A BioVision brand Catalase Activity Colorimetric/Fluorometric Assay Kit was used with the Colorimetric method to determine the catalase in the tissue. The reading was performed with a BMG LABTECH 96 Microplate Reader at 570 nm. We used a Beckman Coulter AU640 brand auto-analyzer applique and a Randox Ransod brand commercial kit to determine the SOD activity in the tissue.
9
Enzymatic Activities in Mouse Skin
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Skin tissues from mice were lysed, and the activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) were measured using the superoxide dismutase 1 (SOD1) (Mouse) ELISA Kit, catalase activity colorimetric/fluorometric assay kit, and glutathione peroxidase activity colorimetric assay kit (BioVision Inc., Milpitas, CA, USA), respectively.
10
Cell Proliferation and Catalase Assay
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Cell proliferation and catalase activity were analyzed using Quick Cell Proliferation Colorimetric Assay Kit (BioVision) and Catalase Activity Colorimetric/Fluorometric Assay Kit (BioVision) according to the manufacturer’s instructions.
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