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Cell lab quanta sc flow cytometer

Manufactured by Beckman Coulter
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The Beckman Coulter Cell Lab Quanta SC is a flow cytometer that measures and analyzes the physical and biochemical characteristics of cells or other particles in a fluid suspension. It is designed to rapidly analyze multiple parameters of single cells flowing through a laser beam. The Quanta SC provides high-resolution data on cell size, granularity, and fluorescence intensity.

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Lab products found in correlation

Fitc conjugated anti brdu antibody, by BD (2 mentions) Fitc annexin 5 apoptosis detection kit, by BD (2 mentions) Kaluza analysis 1, by Beckman Coulter (2 mentions) Kaluza analysis software, by Beckman Coulter (2 mentions) Annexin 5 fitc propidium iodide apoptosis detection kit, by Keygen Biotech (1 mentions) Fitc brdu flow kit, by BD (1 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions) Modfit program, by Verity Software House (1 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions) Model 295e, by PerkinElmer (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) White 96 well plate, by Corning (1 mentions) Annexin 5 and 7 aad, by BioLegend (1 mentions) Caspase glo 3 7 assay, by Promega (1 mentions) Ab187284, by Abcam (1 mentions) Ab33858, by Abcam (1 mentions) Annexin 5, by BD (1 mentions) Aldefluor kit, by STEMCELL (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Rnase a, by Solarbio (1 mentions)

33 protocols using cell lab quanta sc flow cytometer

1

Cell Cycle and Apoptosis Analysis by Flow Cytometry

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For cell cycle analysis, cells were harvested by trypsinization, washed once in ice-cold 1X PBS, centrifuged at 300× g; the resulting cell pellets were fixed in 75% ethanol. Fixed cells were subsequently stained with 250 μL of propidium iodide (PI) solution (20 μg/mL PI, 200 μg/mL DNase-free RNase A in 1X PBS) for 30 min at 37 °C. DNA content was analyzed using a Cell LabQuanta SC flow cytometer (Beckman Coulter Inc., Brea, CA, USA). For apoptosis analysis, cells were stained with the Apoptosis Assay Kit (Invitrogen, Carlsbad, CA, USA), followed by labeling with Alexa FluorR488 annexin V and propidium iodide. For each sample, 10,000 cells were analyzed immediately using the Cell Lab Quanta™ SC flow cytometer (Beckman Coulter, Brea, CA, USA) and software. Apoptotic cells were visualized with an Olympus IX71 fluorescence microscope.
Jeong Y.S., Lam T.G., Jeong S, & Ahn S.G. (2020). Metformin Derivative HL156A Reverses Multidrug Resistance by Inhibiting HOXC6/ERK1/2 Signaling in Multidrug-Resistant Human Cancer Cells. Pharmaceuticals, 13(9), 218.
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2

Cell Proliferation Assay with Flow Cytometry

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Cancer cell proliferation assay was performed via BrdU incorporation and coupled with flow cytometry analysis as described previously (6 (link),7 (link)). RB-proficient and deficient H1299 and H460 cells and SMAC overexpressing H1299 and H460 cells treated with either PD 0332991 or DMSO were pulse-labeled with BrdU for 2 hours prior to harvest. Cells were fixed in 75% ethanol, pelleted, re-suspended in 2N HCl + 0.5 mg/mL pepsin, and incubated at room temperature for 30 minutes. Sodium tetraborate (0.1 mol/L) was added to neutralize 2N HCl. The cell pellets were washed with IFA buffer, centrifuged, and then washed with IFA + 0.5% Tween 20 solution. The pellet was resuspended in IFA solution containing FITC conjugated anti-BrdU antibody (BD Bioscience), incubated in the dark at room temperature for 45 minutes, washed with IFA + 0.5% Tween-20 and pelleted. The pellet was then suspended in PBS containing propidium iodide (0.2 μg/μL) and analyzed for BrdU incorporation using Beckman Coulter Cell Lab Quanta SC flow cytometer. Flow cytometry analysis was performed using FlowJo software (GE Healthcare, Piscataway, NJ, USA).
Thangavel C., Boopathi E., Liu Y., McNair C., Haber A., Perepelyuk M., Bhardwaj A., Addya S., Ertel A., Shoyele S., Birbe R., Salvino J.M., Dicker A.P., Knudsen K.E, & Den. R.B. (2018). Therapeutic Challenge with a CDK 4/6 Inhibitor Induces an RB-dependent SMAC Mediated Apoptotic Response in Non-Small Cell Lung Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(6), 1402-1414.
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3

Quantifying Podocyte Apoptosis

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Apoptosis of the podocytes was assessed 48 h after exposure to various treatments (NG, MA, HG, HG + Sam68-siRNA and Con-siRNA). Apoptosis was determined with an Annexin V-FITC/propidium iodide (PI) apoptosis detection kit according to the manufacturer's instructions (Nanjing KeyGEN Biotech. Co., Ltd.). Briefly, podocytes were trypsinized and centrifuged at 878 × g for 3 min at room temperature. The cells were then suspended in 1X binding buffer and incubated with 5 µl Annexin V-FITC and 5 µl PI in the dark for 10 min. Cell fluorescence was then assessed by using a Cell Lab Quanta™ SC flow cytometer (Beckman Coulter, Inc., Brea, CA, USA). Cells that stained positive for Annexin V-FITC and negative for PI were considered apoptotic.
Chen Y., Zhang L., Liu S., Yao B., Zhang H., Liang S., Ma J., Liang X, & Shi W. (2019). Sam68 mediates high glucose-induced podocyte apoptosis through modulation of Bax/Bcl-2. Molecular Medicine Reports, 20(4), 3728-3734.
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4

BrdU Cell Cycle Analysis by Flow Cytometry

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Cells were pulsed with 10 μM bromodeoxyuridine (BrdU) for one hour. Cells were then fixed and stained for BrdU and DNA content with an anti-BrdU FITC-conjugated antibody and with a 7-aminoactinomycin D (7-AAD) dye, respectively, according to the instructions of the BD Pharmingen FITC BrdU Flow Kit (BD Biosciences FITC BrdU Flow Kit 559619, 557891). All data were collected using the BD FACSCalibur software, and results were analyzed by flow cytometry (Cell Lab Quanta™ SC Flow Cytometer, Beckman Coulter).
Su W.P., Ho Y.C., Wu C.K., Hsu S.H., Shiu J.L., Huang J.C., Chang S.B., Chiu W.T., Hung J.J., Liu T.L., Wu W.S., Wu P.Y., Su W.C., Chang J.Y, & Liaw H. (2017). Chronic treatment with cisplatin induces chemoresistance through the TIP60-mediated Fanconi anemia and homologous recombination repair pathways. Scientific Reports, 7, 3879.
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5

Apoptosis Assay of Drug-Treated Cells

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Cells were plated at 200,000 cells/well in 6-well plates, starved, and treated for 48 hr with drugs. Apoptosis was determined using FITC Annexin V Apoptosis Detection Kit 1 (556547; BD Pharmingen), and analyzed by flow cytometry using a Cell Lab Quanta SC Flow Cytometer (Beckman Coulter). About 10,000 gated events were collected per treatment. Results were calculated using the Mod-fit program (Verity Software House).
Borcherding D.C., Tong W., Hugo E.R., Barnard D.F., Fox S., LaSance K., Shaughnessy E, & Ben-Jonathan N. (2015). Expression and Therapeutic Targeting of Dopamine Receptor-1 (D1R) in Breast Cancer. Oncogene, 35(24), 3103-3113.
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6

Cultivation and Enumeration of P. gingivalis

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P. gingivalis strain W50 (ATCC 53978) was obtained from the culture collection of the Oral Health Cooperative Research Centre at the Melbourne Dental School. P. gingivalis W50 was grown and harvested as described (O'Brien-Simpson et al., 2000 (link); Lam et al., 2016 (link)). Growth conditions of batch cultures were monitored at 650 nm using a spectrophotometer (model 295E, Perkin-Elmer, Germany). As before (Cecil et al., 2016 (link)), cells were harvested during late exponential growth by centrifugation (7,000 g, 20 min at 4°C) and enumerated (number/ml) by flow cytometry using a Cell Lab Quanta SC flow cytometer (Beckman Coulter, Australia) and a LIVE/DEAD BacLight™ Bacterial Viability Kit (Life Technologies, Australia). Bacteria were resuspended in 0.01 M phosphate buffered saline (PBS, Sigma-Aldrich), pH 7.4, before incubation with macrophages. Aliquots of P. gingivalis were heat-killed at 70°C for 1 h, as described (Palm et al., 2013 (link)).
Fleetwood A.J., Lee M.K., Singleton W., Achuthan A., Lee M.C., O'Brien-Simpson N.M., Cook A.D., Murphy A.J., Dashper S.G., Reynolds E.C, & Hamilton J.A. (2017). Metabolic Remodeling, Inflammasome Activation, and Pyroptosis in Macrophages Stimulated by Porphyromonas gingivalis and Its Outer Membrane Vesicles. Frontiers in Cellular and Infection Microbiology, 7, 351.
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7

Annexin V-FITC/PI Cell Apoptosis Assay

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Cells from different conditions were trypsinized and combined with cells in the medium by centrifugation. Harvested cells were washed with PBS and incubated with annexin V binding buffer containing annexin V-FITC/propidium iodide (PI, 556547, BD) at room temperature for double staining for 15 min. Flow cytometry was used to analyze cell apoptosis with a Cell Lab Quanta SC Flow Cytometer (Beckman Coulter). The experiment was repeated for three times.
Shieh J.M., Shen C.J., Chang W.C., Cheng H.C., Chan Y.Y., Huang W.C., Chang W.C, & Chen B.K. (2014). An Increase in Reactive Oxygen Species by Deregulation of ARNT Enhances Chemotherapeutic Drug-Induced Cancer Cell Death. PLoS ONE, 9(6), e99242.
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8

Apoptosis Quantification by Flow Cytometry

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Cells were plated at 300,000 cells/well in 6-well plates. For inhibitor studies, ODQ or KT5823 was added 1 h before treatment with other drugs. After 72 h, apoptosis was determined using FITC/Annexin V Apoptosis Detection Kit (BD Biosciences, San Jose, CA). Briefly, cells were incubated with Annexin V and propidium iodide for 15 m and then analyzed by flow cytometry using a Cell Lab Quanta SC Flow Cytometer with accompanying software (Beckman Coulter). About 10,000 gated events were collected per treatment.
Tuttle T.R., Mierzwa M.L., Wells S.I., Fox S.R, & Ben-Jonathan N. (2015). The cyclic GMP/protein kinase G pathway as a therapeutic target in head and neck squamous cell carcinoma. Cancer letters, 370(2), 279-285.
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9

Cell Cycle Analysis by Flow Cytometry

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Twenty-four hours after treatment of B16 cells with concentrations of 1 and 5 μM of each compound, cells were harvested and fixed with pre-chilled 70% ethanol solution at −20 °C. Cells were incubated with 0.2 mg/mL RNAse and 20 μg/mL propidium iodide (PI) in a 0.1% Triton TX-100 solution, in the dark, for 30 min at 37 °C. Cell cycle distribution was analyzed using a Beckman Coulter Cell Lab Quanta SC Flow Cytometer (Indianapolis, IN, USA), and the data were analyzed using the Quanta Analysis software.
Olar R., Maxim C., Badea M., Bacalum M., Raileanu M., Avram S., Korošin N.Č., Burlanescu T, & Rostas A.M. (2022). Antiproliferative Copper(II) Complexes Bearing Mixed Chelating Ligands: Structural Characterization, ROS Scavenging, In Silico Studies, and Anti-Melanoma Activity. Pharmaceutics, 14(8), 1692.
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10

Caspase-3/7 and Apoptosis Assays

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Cells were treated with a 1:4 dilution series ranging from 20 µM to 0.078 µM LMK-235 in 96-well cell polystyrene cell culture plates (Greiner Bio-One). Caspase-Glo 3/7 assay (Promega, Mannheim, Germany) was performed after 0, 8, 24, and 32 h according to the manufacturer’s protocol. Supernatants including the reaction mixture were transferred for luminescence measurements into white 96-well plates (Corning, Kaiserslautern, Germany). Luminescence measurements were performed on the Infinity M200 reader with an integration time of 5 s.
After 24 h incubation with a LMK-235 1:4 dilution series (0.078–20 µM) in 6-well polystyrene cell culture plates (TPP), cells were harvested and stained according to the manufacturer’s instructions with Annexin-V and 7-AAD (both BioLegend, Koblenz, Germany) for FACS analysis of apoptosis on a Cell Lab Quanta SC flow cytometer (Beckman-Coulter, Vienna, Austria) and the Kaluza Analysis 1.3 software (Beckman-Coulter).
Wanek J., Gaisberger M., Beyreis M., Mayr C., Helm K., Primavesi F., Jäger T., Di Fazio P., Jakab M., Wagner A., Neureiter D, & Kiesslich T. (2018). Pharmacological Inhibition of Class IIA HDACs by LMK-235 in Pancreatic Neuroendocrine Tumor Cells. International Journal of Molecular Sciences, 19(10), 3128.
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