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Progres capturepro software

Manufactured by Jenoptik
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Sourced in Germany, Japan

ProgRes CapturePro software is a tool for capturing and managing digital images. It provides a user interface for controlling connected cameras and saving the captured images to a computer.

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Lab products found in correlation

Progres cfscan camera, by Jenoptik (3 mentions) Dm irb microscope, by Leica (3 mentions) N plan 10 0.25 numerical aperture objective lens, by Leica (2 mentions) Progres mf camera, by Jenoptik (2 mentions) Bx60 microscope, by Olympus (2 mentions) Progres c14 plus camera, by Jenoptik (2 mentions) Haematoxylin gill 3, by Leica (2 mentions) Eosin y, by Leica (2 mentions) Progres c10plus camera, by Jenoptik (1 mentions) Thms600, by Linkam (1 mentions) Axioscope 7 microscope, by Zeiss (1 mentions) Zen software, by Zeiss (1 mentions) Lsm 780, by Zeiss (1 mentions) Optical microscope, by Olympus (1 mentions) Tissue tek oct compound, by Electron Microscopy Sciences (1 mentions) Vectashield mounting media with dapi, by Vector Laboratories (1 mentions) Axioplan 2 fluorescence microscope, by Zeiss (1 mentions) Progres cf cool ccd camera, by Jenoptik (1 mentions) Hematoxylin, by Vector Laboratories (1 mentions) Biotinylated goat anti rabbit, by Jackson ImmunoResearch (1 mentions)

24 protocols using progres capturepro software

1

Detecting β-galactosidase Expression in Hearts

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β-galactosidase expression was detected by X-gal staining. Embryonic, neonatal or adult hearts were dissected in cold phosphate-buffered saline (PBS) and fixed in 2% paraformaldehyde (PFA), 0.2% glutaraldehyde in PBS at 4 °C for 30 min to 2 h, depending on age, followed by two washes in Rinse solution (2 mM MgCl2, 0.2% Nonidet P40, 0.1% sodium deoxycholate in PBS). They were then incubated in Staining solution (Rinse solution containing 1 mg/ml 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-gal), 5 mM K4Fe(CN)6 and 5 mM K3Fe(CN)6) at room temperature for 3 h to overnight, depending on staining intensity and age of sample. Imaging of whole hearts was performed using a stereo microscope (Leica M165C) equipped with a ProgRes CF Scan camera and ProgRes CapturePro software (Jenoptik).
For samples at embryonic day (E)13 and younger, following whole-mount imaging the X-gal-stained hearts were dehydrated through an ethanol series, cleared using Histo-Clear (National Diagnostics) and paraffin-embedded for sectioning. Sections measuring 10 μm were de-waxed, counter-stained using Nuclear Fast Red (Electron Microscopy Services) and imaged using an Axioplan 2 upright microscope (Zeiss) with a ProgRes C5 camera and ProgRes CapturePro software (Jenoptik).
Payne S., Gunadasa-Rohling M., Neal A., Redpath A.N., Patel J., Chouliaras K.M., Ratnayaka I., Smart N, & De Val S. (2019). Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart. Nature Communications, 10, 3276.
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2

Isolation and Differentiation of Murine Osteoclasts

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Mouse bone marrow cells were isolated from tibiae and femurs of 6-week-old ICR mice by flushing the bone marrow with α-minimal essential medium (α-MEM; Hyclone Lab Inc., Logan, UT, USA). Bone marrow cells were cultured for 3 days in α-MEM (Hyclone Lab Inc.) containing 10% fetal bovine serum (FBS; Hyclone Lab Inc.) in the presence of M-CSF (30 ng/mL). Adherent cells were further cultured in the presence of M-CSF (30 ng/mL) and RANKL (150 ng/mL) for 3 days. Cultured cells were fixed with 3.7% formalin and stained for tartrate-resistant acid phosphatase (TRAP). TRAP-positive cells with more than 3 nuclei were considered TRAP-positive MNCs. Cells were observed using a Leica DMIRB microscope equipped with an N Plan 10×0.25 numerical aperture objective lens (Leica Microsystems, Wetzlar, Germany). Images were captured with a ProgRes CFscan camera (Jenoptik, Jena, Germany) using ProgRes Capture Pro software (Jenoptik).
Kim J.H., Yang Y.R., Kwon K.S, & Kim N. (2021). Anti-Müllerian Hormone Negatively Regulates Osteoclast Differentiation by Suppressing the Receptor Activator of Nuclear Factor-κB Ligand Pathway. Journal of Bone Metabolism, 28(3), 223-230.
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3

Histological Analysis of Liver and Intestine

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Samples of liver and intestine fixed in 10% buffered formalin were dehydrated in a graded ethanol series and embedded in paraffin. Sections of 4 mm were stained with haematoxylin and eosin (H&E) (Martoja and Martoja-Pierson, 1970) for morphological observations using a Leica DM5000B microscope (Jenoptik, Germany). Images were taken with ProgRes® CapturePro software (Jenoptik, Germany).
Trullàs C., Fontanillas R., Tres A., Barroeta A.C, & Sala R. (2016). Acid and re-esterified rapeseed oils as alternative vegetable oils for rainbow trout diets: Effects on lipid digestibility and growth. Aquaculture (Amsterdam, Netherlands), 451.
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4

Quantifying Drosophila Egg Size

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Females were incubated with fresh ground yeast in the presence of males for ~24 hours prior to moving them to apple juice plates with wet yeast. Mature eggs were collected at the indicated times and temperatures (Table S1), rinsed from apple juice plates with distilled water, and imaged using the 10x objective on a standard Zeiss compound microscope equipped with a ProgRes MF camera (Jenoptik). Images were captured using the ProgRes Capture Pro software (Jenoptik). Fiji/ImageJ was utilized to measure the length and width of the mature eggs, and the following formula was used to calculate the volume of the mature eggs: Volume = 1/6(π)(width)2(length) as done previously (Kline et al., 2018 (link)).
Thestrup J., Tipold M., Kindred A., Stark K., Curry T, & Lewellyn L. (2020). The Arp2/3 complex and the formin, Diaphanous, are both required to regulate the size of germline ring canals in the developing egg chamber. Developmental biology, 461(1), 75-85.
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5

Starch Birefringence Microscopy Analysis

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Samples of biscuits (i.e., ground to crumbs) and flour ingredients were suspended in deionised water and examined directly using an Olympus BX60 microscope (Olympus Corporation, USA) under polarised light to display starch birefringence. Images were captured with a ProgRes® C10 plus camera (Jenoptik, Germany) and ProgRes® CapturePro software (Jenoptik, Germany). Starch was stained with 1% iodine-potassium iodide (w/w).
Heated stage polarised microscopy was used to monitor changes in ordered structure during hydrothermal treatment. Composite flours (20 mg) were suspended in excess deioinised water (400 µL), mixed by inversion, and then 50 µL of this suspension mounted on microscope slides, sealed, and then viewed under polarised light while being heated from 25 °C to 80 °C in excess water on a heated-stage (THMS600, Linkam Scientific Instruments Ltd, UK). Images were captured at regular intervals (with LINK System Software, Linkam Scientific Instruments Ltd, UK) to observe changes in starch birefringence.
Delamare G.Y., Butterworth P.J., Ellis P.R., Hill S., Warren F.J, & Edwards C.H. (2020). Incorporation of a novel leguminous ingredient into savoury biscuits reduces their starch digestibility: Implications for lowering the Glycaemic Index of cereal products. Food Chemistry: X, 5, 100078.
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6

DIC Imaging and Lipid Droplet Analysis

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Differential interference contrast (DIC) imaging was done for all replicates from the table with an Olympus BX-60 microscope (Olympus, Japan) with a ProgRes C14plus camera and the ProgRes CapturePro Software (version 2.9.01) (JENOPTIK AG). The morphology of chosen conditions (Fig. 1, Extended Data Figs. 4–6 and Supplementary Fig. 1) of Mesotaenium cells that were 89 h on the table was analysed.
For algae that were used for quantifying the abundance of LD per cell, a ZEISS Axioscope 7 microscope (Carl Zeiss) was used including the Zen software (Carl Zeiss). The LD count was carried out in Fiji159 (link). For statistical analysis of the LD count data, we first used a Shapiro–Wilk test160 (link) to assess normality and used Mann–Whitney U tests161 (link) with R (version 3.6.1) accordingly.
Confocal laser scanning microscope was done on a Zeiss LSM780 (Carl Zeiss) set as in Müller et al.162 (link). For the staining of the LD structures, we used the neutral lipid specific stain BODIPY 493/503 (EM/EX) (Merck). Mesotaenium cells were grown for 22 days on WHM medium at 70–80 µmol photons m−2 s−1 and 22 °C. These cells were ultrasonicated for 1 min with 1:500 BODIPY and incubated on a shaker for 5 min before visualization.
Dadras A., Fürst-Jansen J.M., Darienko T., Krone D., Scholz P., Sun S., Herrfurth C., Rieseberg T.P., Irisarri I., Steinkamp R., Hansen M., Buschmann H., Valerius O., Braus G.H., Hoecker U., Feussner I., Mutwil M., Ischebeck T., de Vries S., Lorenz M, & de Vries J. (2023). Environmental gradients reveal stress hubs pre-dating plant terrestrialization. Nature Plants, 9(9), 1419-1438.
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7

Histomorphometric Analysis of Jejunum Tissue

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Jejunum segments were fixed with 4% paraformaldehyde and embedded in paraffin after 48 h. For morphological examination, tissue sections (5 μm) were stained with hematoxylin and eosin. Nine complete villi were measured in each section. The villus height was measured from the tip of the villus to the villus-crypt junction, and crypt depth was measured from the bottom of the villus to the lamina propria. Then, the villus height/crypt depth ratio was calculated. All the observations and measurements were performed with an Olympus optical microscope and ProgRes CapturePro software (version 2.7; Jenoptik, Jena, Germany).
Huang L., Luo L., Zhang Y., Wang Z, & Xia Z. (2018). Effects of the Dietary Probiotic, Enterococcus faecium NCIMB11181, on the Intestinal Barrier and System Immune Status in Escherichia coli O78-Challenged Broiler Chickens. Probiotics and Antimicrobial Proteins, 11(3), 946-956.
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8

Measuring Drosophila Egg Morphology

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Female flies of the appropriate genotype were incubated with ground yeast in the presence of males for ∼24 h before being transferred to apple juice plates with wet yeast. The apple juice plates were replaced every ∼24 h. Mature eggs were collected from the apple juice plates and imaged using a Zeiss-LP520 microscope with a ProgRes MF camera (Jenoptik) using ProgRes Capture Pro Software (Jenoptik). The lengths and widths of the eggs were determined using the line tool in Fiji/ImageJ. The volume was calculated using the equation volume=1/6π(length)(width)2. Embryonic viability was assessed by placing mature eggs on fresh apple juice plates and recording the percentage that has hatched after >24 h at 25°C.
Stark K., Crowe O, & Lewellyn L. (2021). Precise levels of the Drosophila adaptor protein Dreadlocks maintain the size and stability of germline ring canals. Journal of Cell Science, 134(8), jcs254730.
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9

Histomorphological Analysis of Jejunum

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On d 21, the midregions of the jejunum (approximately 1 cm) were collected in 4% paraformaldehyde solution and then embedded in paraffin. Transverse 5-μm sections were stained with hematoxylin and eosin, and histomorphological parameters were examined using an Olympus optical microscope and ProgRes Capture Pro Software (version 2.7, Jenoptik, Jena, Germany). The villus height and crypt depth of the slice samples were read, and the ratio of villus height to crypt depth was calculated. Ten complete and vertical villi were selected for each slice sample. The villus height is the height from the top of the villus to the crypt opening, and the crypt depth is the distance from the crypt opening to the base of the crypt.
Zhao Y., Fu J., Li P., Chen N., Liu Y., Liu D, & Guo Y. (2021). Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens. Poultry Science, 101(1), 101553.
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10

Histological Analysis of Cardiac Tissue

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Hearts were harvested at the time points specified and flash frozen in Tissue-Tek OCT compound (Electron Microscopy Sciences; Hatfield, PA) using liquid nitrogen. Frozen hearts were sectioned at 12 μm thickness. For Picro-Sirius Red: Sections were incubated Picro-Sirius Red solution and then rinsed with 0.5% acetic acid and 100% ethanol, sequentially. For Oil Red O: Sections were fixed with 4% PFA and rinsed with 60% isopropanol, and then incubated with freshly filtered Oil Red O solution at room temperature. Sections were mounted with VECTASHIELD® Mounting Media with DAPI (Vector Laboratories; Burlingame, CA), and visualized with ProGres Capture Pro Software (Jenoptik; Jena, Germany) using a Leica DM2000 microscope equipped with a Jenoptik ProgRes C14plus camera.
Mazurek S.R., Calway T., Harmon C., Farrell P, & Kim G.H. (2017). MicroRNA-130a Regulation of Desmocollin 2 in a Novel Model of Arrhythmogenic Cardiomyopathy. MicroRNA (Shariqah, United Arab Emirates), 6(2), 143-150.
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