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5 protocols using periodate

1

DMEM-based Cell Culture Protocol

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Dulbecco’s Modified Eagle’s Medium high Glucose (DMEM), Fetal Bovine Serum (FCS), L-Glutamine, Penicillin-streptomycin (pen-strep) Dulbecco’s PBS were all purchased from biological industries; Trizma-hydrochloride, Tween-20, Ovalbumin (Grade V), Ophenylenediamine (OPD) and periodate were all purchased from Sigma-Aldrich, EDTA was purchased from Fisher Scientifics, Hydrogen peroxide 30% was purchased from Merck.
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2

Phage Adsorption Modulation by Proteinase K and Periodate

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To test whether proteinase K or periodate affects phage adsorption, K. pneumoniae isolates were pretreated with 20 μg/mL proteinase K (Solarbio, China), 100 mM periodate (Sigma, USA), and 50 mM sodium acetate (Sigma) for 3 h at 37°C and then coincubated with phage at room temperature for 5 min. The residual phage proportion was calculated as the percentage of phage quantities after proteinase K, periodate, or lipopolysaccharides treatment compared to the initial phage quantities. To further validate the phage inactivation caused by LPS, the LPS were extracted from host strains using an LPS extraction kit (catalog no. 17141; Intron Biotechnology, South Korea) and used in a phage inactivation test as previously described (37 (link)). Phages treated with LPS of Escherichia coli O111:B4 (Sigma) were used as a control group.
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3

Phage Adsorption Modulation by Proteinase K and Periodate

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To test whether proteinase K or periodate affects phage adsorption, K. pneumoniae isolates were pretreated with 20 μg/mL proteinase K (Solarbio, China), 100 mM periodate (Sigma, USA), and 50 mM sodium acetate (Sigma) for 3 h at 37°C and then coincubated with phage at room temperature for 5 min. The residual phage proportion was calculated as the percentage of phage quantities after proteinase K, periodate, or lipopolysaccharides treatment compared to the initial phage quantities. To further validate the phage inactivation caused by LPS, the LPS were extracted from host strains using an LPS extraction kit (catalog no. 17141; Intron Biotechnology, South Korea) and used in a phage inactivation test as previously described (37 (link)). Phages treated with LPS of Escherichia coli O111:B4 (Sigma) were used as a control group.
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4

Biodiesel Production Catalyst Evaluation

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The raw materials employed to produce biodiesel, degummed soy oil, methanol (99.9%), and pure glycerol (>99.5%), were supplied by Sigma. Sodium stearate (99%) was chosen as the soap model and supplied by Serisa Química SRL. Glyceryl monostearate (98%) was used as the monoglyceride model and supplied by Cloretil SACIF. Silica samples were TriSyl 3000, 300B and 450 (provided by W.R. Grace Argentina SA). TEOS (99% purity) and periodate were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received. Chitosan (deacetylation degree of 80%) was purchased from TCI-America (Portland, OR, USA).
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5

Spectrophotometric Analysis of Anionic Salts

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Rosmarinic acid, sodium borohydride, ascorbic
acid, HEPES buffer, sodium phosphate monobasic, and agarose (low melting
point) were purchased from Sigma-Aldrich. AgNO3 was purchased
from S. D. Fine Chemicals. Tetrabutylammonium (TBA+) salts
of fluoride, bromide, chloride, iodide, periodate, cyanide, dihydrogen
phosphate, acetate, nitrate, and bisulfate were purchased from Sigma
Aldrich. Potassium dichromate was purchased from Merck Ltd. Solutions
of all the chemicals were prepared in Milli-Q water.
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