Anti ubiquitin z0458

Antibodies were from the following companies; mouse mAb anti-myostatin (MstnPP) (6H12) (Abcam & ThermoFischer Scientific); goat pAb anti-human myostatin (amino acid residues 268–376) (R & D systems); mouse mAb anti-APP 6E10 against Aβ epitope RHDSGYE (BioLegend); mouse mAb anti-APP 22C11 against the aminoterminal residues 66–81 (Merck Millipore); rabbit pAb anti-Giantin ab24586 (Abcam); rabbit pAb anti-LC3B NB100-2220 (Novus biological); rabbit pAb anti-Lamp1 ab24170 (Abcam); rabbit pAb anti-GRP-78 H-129 (Santa Cruz); rabbit pAb anti-GFP A-6455 (ThermoFischer Scientific); rabbit mAb anti-Calreticulin ERP3924 (Merck Millipore); rabbit pAb anti-Calnexin C4731 (Sigma); rabbit pAb anti-Ubiquitin Z0458 (DAKO); mouse mAb anti-Actin (MP Biomedicals); Alexa Fluor-conjugated secondary antibodies (Molecular Probes); HRP-coupled secondary goat antibodies (Dianova). Chemicals were purchased from Sigma or Roth.

Cell culture reagents including high glucose DMEM (Dulbecco’s Modified Eagle Medium), (β-D-arabinofuranosyl cytosine, D-glucose, ploy L-lysine, BCA protein estimation kit), and primary antibodies such as mouse monoclonal anti-β-actin (A5316), anti-Ube3a (E8655), and anti-Tuj1 (T8660) were purchased from Sigma. Fetal bovine serum (FBS), trypsin (0.25%), penicillin-streptomycin, neurobasal medium, minimum essential medium, Lipofectamine^®2000, Opti-MEM, sodium pyruvate were procured from Gibco/Thermo Fisher Scientific. Mouse monoclonal anti-Ube3a was purchased from Santa Cruz Biotechnology (SC-16689) and BD Bioscience (611416). Rabbit monoclonal anti-H3(4499), anti-H3(K9) (9649), anti-H4(13919), anti-H4(K12) (2591) and anti-RAD23A; mouse monoclonal anti-HDAC1(5356) and anti-HDAC2(5113) were purchased from Cell Signaling Technology. Rabbit polyclonal anti-BDNF (NBP1-59304) was from Novus Biologicals and anti-ubiquitin (Z0458) was from DAKO. Horseradish peroxidase (HRP) conjugated and biotinylated secondary antibodies, VectaStain ABC kit, ImmPACT NovaRED HRP substrate kits were purchased from Vector laboratories. Ube3a siRNA and control siRNA were procured from Santa Cruz Biotechnology.