Signaling studies in MEFs were performed as described19 (link). RIPK1 immunoprecipitations were performed using anti-RIPK1 (Cell Signaling) and Dynabeads M270 Epoxy (Invitrogen). K63-ubiquitin immunoprecipitations were performed using anti-K63 (Millipore) and Dynabead Protein A (Invitrogen). Studies of RIPK3 mutants in A20−/−Ripk3−/− MEFs were performed by generating RIPK3 lysine mutants, introducing mutant RIPK3-encoding cDNAs into GFP expressing lentiviral constructs, and infecting A20−/−Ripk3−/− MEFs with these viruses. Productively infected cells were FACS-sorted to obtain pure populations of RIPK3-expressing cells prior to being tested in necroptosis assays. Cell survival of MEFs was quantitated using the CellTiter-Glo Luminescent Cell Viability Assay per manufacturer’s instructions (Promega).
Anti a20
Anti-A20 is a laboratory reagent that can be used to detect and quantify the A20 protein in various biological samples. A20 is a key regulator of inflammatory signaling pathways. This product can be used in applications such as Western blotting, immunoprecipitation, and ELISA to study the expression and function of A20 in cells and tissues.
Lab products found in correlation
7 protocols using anti a20
RIPK1-Mediated Necroptosis Signaling
Ubiquitin-mediated protein regulation assay
Evaluating CD40-mediated Activation in U2OS and iDCs
Protein Extraction and Western Blot Protocol
RIPK1-Mediated Necroptosis Signaling
Signaling studies in MEFs were performed as described19 (link). RIPK1 immunoprecipitations were performed using anti-RIPK1 (Cell Signaling) and Dynabeads M270 Epoxy (Invitrogen). K63-ubiquitin immunoprecipitations were performed using anti-K63 (Millipore) and Dynabead Protein A (Invitrogen). Studies of RIPK3 mutants in A20−/−Ripk3−/− MEFs were performed by generating RIPK3 lysine mutants, introducing mutant RIPK3-encoding cDNAs into GFP expressing lentiviral constructs, and infecting A20−/−Ripk3−/− MEFs with these viruses. Productively infected cells were FACS-sorted to obtain pure populations of RIPK3-expressing cells prior to being tested in necroptosis assays. Cell survival of MEFs was quantitated using the CellTiter-Glo Luminescent Cell Viability Assay per manufacturer’s instructions (Promega).
THP-1 Cells Immunoblotting Protocol
Immunoblotting of Apoptosis Regulators
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