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Retiga exi

Manufactured by Zeiss
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The Retiga ExI is a high-performance, cooled CCD camera designed for scientific imaging applications. It features a large sensor area, high quantum efficiency, and low noise to capture detailed and sensitive images. The core function of the Retiga ExI is to provide accurate and reliable image capture for a variety of scientific and research applications.

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Lab products found in correlation

Axiovert s100 microscope, by Zeiss (1 mentions) Dyecycle green, by Thermo Fisher Scientific (1 mentions) Axio observer, by Zeiss (1 mentions)

2 protocols using retiga exi

1

Pollen Staining and Imaging Protocol

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Fresh pollen was collected from each plant, immediately fixed in ethanol: acetic acid (3:1 ratio) solution, and later rehydrated through an ethanol series for size measurements and staining. To visualize nuclei, fixed pollen grains were stained with DyeCycle Green (Thermo Fisher Scientific, Cat no. V35004) at a final concentration of 1 μM in water, incubated in dark for 30 min and washed with sterile water. Stained pollen was then imaged on a Zeiss Axiovert S100 microscope equipped with HBO 50 Illuminator and Chroma #41017 filter set (470/40 excitation, 495 long-pass dichroic, 525/50 emission) using Qimaging (Retiga ExI) camera.
Zhou L., Vejlupkova Z., Warman C, & Fowler J.E. (2021). A Maize Male Gametophyte-Specific Gene Encodes ZmLARP6c1, a Potential RNA-Binding Protein Required for Competitive Pollen Tube Growth. Frontiers in Plant Science, 12, 635244.
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2

Whole-mount in situ RNA hybridization for mbp

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Whole-mount in situ RNA hybridization was performed as described (Thisse and Thisse, 2008 ) to detect mbp (Brösamle and Halpern, 2002 (link)). Following hybridization, tissues were fixed with 4% paraformaldehyde, equilibrated in 70% glycerol and mounted on glass coverslips for whole-mount imaging. Images were collected using a Zeiss Axio Observer equipped with DIC optics, Retiga Exi digital color camera and Volocity software. All images were imported into Adobe Photoshop software and image processing was limited to changes in levels, contrast, brightness and cropping.
Blasky A.J., Pan L., Moens C.B, & Appel B. (2014). Pard3 regulates contact between neural crest cells and the timing of Schwann cell differentiation but is not essential for neural crest migration or myelination. Developmental dynamics : an official publication of the American Association of Anatomists, 243(12), 1511-1523.
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