Anti mouse cd4 antibody

For CD4⁺ T cells depletion, mice were inoculated intraperitoneally with 500 μg of anti-mouse CD4 antibody (Clone: GK1.5; Bio X Cell) at −1 and 4 dpi. For GM-CSF depletion, mice were inoculated intraperitoneally with 250 μg of anti-mouse GM-CSF antibodies (Clone: MP1-22E9; Bio X Cell) at 4 dpi. For IFNγ depletion, mice were inoculated intraperitoneally with 500 μg of anti-mouse IFNγ antibody (Clone: XMG1.2; Bio X Cell) at 0, 2, and 4 dpi. All drugs given were prepared in 100 μL volume diluted in sterile PBS. Control animals were given 100 μL of PBS, to ensure that in these animals, the infection will resemble as closely as possible to non-treated animals. Appendix Fig. S1 shows that disease severity and viral load clearance were comparable between animals receiving PBS and isotype control antibodies. Macrophages were depleted with clodronate liposomes (LIPOSOMA) given intravenously at 5 dpi. Clodronate liposomes were given at a final concentration of 1 mg/mL, in a volume of 200 μL. Control animals were given empty liposomes (LIPOSOMA).

Unless otherwise specified, 100,000 breast cancer cells were injected s.c. adjacent to the inguinal mammary fat pad of female WT, Rag1^KO, Batf3^KO, Ifnar1^KO, Sting^KO, Ticam1^KO Myd88^KO, Ccr5^KO Cxcr3^KO, Ifnar1^KO, and Cxcr3 ^KO mice on the C57BL/6 background. 500,000 D4M.3A.3 melanoma and melanoma-MT cells were injected subcutaneously into the flanks of DMBA3-4-immunized and naive mice on the C57BL/6 background. In the LLC tumor model, 1,000,000 LLC cells were injected s.c. into flanks of naive mice on the C57BL/6 background. For macrophage and T cell depletion, clodronate liposome (catalog no. PBS-02, Liposoma BV), anti-mouse CD4 antibody (catalog no. BE0003, Bio X Cell), and anti-mouse CD8β antibody (catalog no. BE0223, Bio X Cell) were i.p. injected once a week, respectively. For natural killer cell depletion, Ncr1^iCre, ROSA^DTR mice were treated with diphtheria toxin (catalog no. D0564, Sigma-Aldrich) as well as anti-NK1.1 antibody (catalog no. BE0036, Bio X Cell). For DC depletion, CD11c-DTR mice were treated with diphtheria toxin. Tumor growth was monitored daily and measured on regular intervals. When a tumor reached 2 cm in diameter or became ulcerated, it was deemed terminal. In the lung metastasis mouse model, 500,000 DMBA3-4 versus DMSO3-1 cells were i.v. injected into tail vein of WT mice on the C57BL/6 background.