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Galactose oxidase from dactylium dendroides

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Galactose oxidase from Dactylium dendroides is an enzyme that catalyzes the oxidation of galactose to D-galactohexodialdose. It is a useful tool for the analysis and detection of galactose in various biological samples.

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Lab products found in correlation

D galactose, by Merck Group (2 mentions) Galactose, by Merck Group (1 mentions) Laccase from trametes versicolor, by Merck Group (1 mentions) Horseradish peroxidase hrp, by Merck Group (1 mentions) 2 2 azino bis, by Merck Group (1 mentions) Ferricyanide, by Merck Group (1 mentions) Ferrocyanide, by Merck Group (1 mentions) Hydrogen peroxide, by Avantor (1 mentions) Acetaminophen, by Merck Group (1 mentions) Glucose oxidase gox from aspergillus niger, by MP Biomedicals (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Chitosan, by Merck Group (1 mentions) Nafion, by Merck Group (1 mentions) Ninhydrin, by Merck Group (1 mentions) Ribonuclease b, by Merck Group (1 mentions) 10 acetyl 3 7 dihydroxyphenoxazine, by Merck Group (1 mentions) Pngase f, by Merck Group (1 mentions) Horseradish peroxidase, by Merck Group (1 mentions) Lucirin tpo l, by BASF (1 mentions) Hypermer b246, by Croda (1 mentions)

Close spelling variants of this product

Galactose (363 citations) Galactose oxidase (4 citations)

3 protocols using galactose oxidase from dactylium dendroides

1

Polymer Synthesis and Functionalization

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Methyl acrylate (MA), 2-hydroxyethyl
acrylate (HEA), methyl methacrylate (MMA), 2-hydroxyethyl methacrylate
(HEMA), 2,2′-azobis(2-methylpropionitrile) (AIBN),
methyl 2-bromopropionate (MBP), N,N,N′,N′,N″-pentamethyldiethylenetriamine (PMDETA), tris(2-dimethylaminoethyl)amine
(M6-TREN), CuBr2, Cu0, m-xylene (m-Xi), dimethyl sulfoxide (DMSO), dimethylformamide
(DMF), divinyl sulfone (DVS), ethylene diamine (EDA), dansyl cadaverine
(DC), glutaraldehyde solution (50 wt % in H2O), 10% poly(ethyleneimine)
solution (PEI; average Mn ∼ 60,000
by GPC, average Mw ∼ 750,000 by
LS), potassium phosphate, sodium carbonate, 1% PEI (average Mw ∼ 25,000 by LS, average Mn ∼ 10,000 by GPC), glycine (GLY), galactose, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid) (ABTS), galactose oxidase from Dactylium dendroides, laccase from Trametes versicolor, and horseradish peroxidase (HRP) were purchased from Sigma-Aldrich.
Medina-Castillo A.L., Ruzic L., Nidetzky B, & Bolivar J.M. (2022). Hydrophilic Nonwoven Nanofiber Membranes as Nanostructured Supports for Enzyme Immobilization. ACS Applied Polymer Materials, 4(8), 6054-6066.
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2

Enzyme-Mediated Glucose and Galactose Sensing

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All chemicals were of analytical grade unless noted otherwise and were used as received. Glucose oxidase (GOx) from Aspergillus niger was obtained from MP Biomedicals. Dextrose (D-Glucose) (certified ACS granular powder) was obtained from Fischer Bioreagents. Galactose oxidase from Dactylium dendroides, D-(+)-Galactose (≥99%), Chitosan (from shrimp shells, ≥75% (deacetylated)), 5% Nafion, L-Ascorbic Acid (99%), Acetaminophen (≥99%), ferricyanide (99.98%), and ferrocyanide (≥98.5 %) were obtained from Sigma-Aldrich. Hydrogen peroxide (30 wt%) was purchased from VWR International. Dulbeccos’ Phosphate Buffered Saline (DPBS) (1X) with calcium and magnesium (pH = 6.8) was purchased from Corning. All stock solutions were made with DPBS.
Walker N.L, & Dick J.E. (2021). Oxidase-Loaded Hydrogels for Versatile Potentiometric Metabolite Sensing. Biosensors & bioelectronics, 178, 112997.
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3

Glycan Analysis with Enzyme-Linked Assay

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Reagents Pentaerythritol-tetrakisIJ3-mercaptopropionate) ("tetrathiol"), triallyl-1,3,5-triazine-2,4,6IJ1H,3H,5H)-trione ("triallyl"), 2-(boc amino)ethanethiol, L-ascorbic acid (ASA), galactose-oxidase from Dactylium dendroides [50 U ml -1 ], horseradish peroxidase (HRP, lyophilized powder, 150 U mg -1 ), PNGase F, ninhydrin, D-galactose, D-IJ+)-glucose, ribonuclease B (RNase B from bovine pancreas (50 Kunitz units per mg protein)), 10acetyl-3,7-dihydroxyphenoxazine (ADHP), Tween 20 and 5,5′-dithiobisIJ2-nitrobenzoic acid) (DTNB) were obtained from Sigma Aldrich (Brøndby, DK). Lucirin TPO-L (ethyl-2, 4, 6-trimethylbenzoylphenyl phosphinate) was obtained from BASF (Hardmatt, CH). Sylgard 184 -polyIJdimethylsiloxane) (PDMS) elastomer kit was obtained from Dow Corning (Midland, MI, USA). Hypermer B246 and Span 80 surfactants were obtained from Croda International Plc (Snaith, UK)
Lafleur J.P., Senkbeil S., Novotny J., Nys G., Bøgelund N., Rand K.D., Foret F, & Kutter J.P. (2015). Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors. Lab on a chip, 15(10).
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