The P. indica colonization in wheat roots was identified by staining the root fragments using trypan blue according to the method of Li et al. [53 (link)]. Root samples were then subjected to microscopic observation using a Nikon ECLIPSE NI-U research microscope (Nikon, Tokyo, Japan). For Scanning Electron Microscopy (SEM) observation, the roots of the co-cultured seedlings were cut into small pieces, washed three times in double distilled water, and then soaked in 2.5% glutaraldehyde for 30 min. After several rinses with double distilled water, they were dehydrated in 50, 70, 80, 90, and 100% ethanol step-by-step (15 min each step). The samples were dried overnight at 4 °C, coated with ETD 2000C ion coater (Beijing Fine Technology Development Co., LTD., Beijing, China), and observed under ZEISS MERLIN compact scanning electron microscope (ZEISS, Jena, Germany).
Merlin compact scanning electron microscope
Manufactured by Zeiss
Sourced in Germany
The Merlin Compact is a scanning electron microscope (SEM) designed by Zeiss. It provides high-resolution imaging capabilities for various applications. The core function of the Merlin Compact SEM is to generate detailed images of sample surfaces by scanning them with a focused electron beam.
Automatically generated - may contain errors
Lab products found in correlation
K alpha spectrometer, by Thermo Fisher Scientific (2 mentions)
Jem 3010, by JEOL (1 mentions)
1800 pc uv vis spectroscope, by MAPADA (1 mentions)
Talos f200s transmission electron microscope, by Thermo Fisher Scientific (1 mentions)
D8 advance diffractometer, by Bruker (1 mentions)
Xrd 6000, by Shimadzu (1 mentions)
D8 advance x ray powder diffractometer, by Bruker (1 mentions)
Jem 2100 transmission electron microscope, by JEOL (1 mentions)
Thermo escalab 250xi electron spectrometer, by Thermo Fisher Scientific (1 mentions)
D8 focus powder x ray diffractometer, by Bruker (1 mentions)
Stereo discovery v12, by Zeiss (1 mentions)
Fiji software, by Zeiss (1 mentions)
Zen software, by Zeiss (1 mentions)
8 protocols using merlin compact scanning electron microscope
1
Visualizing P. indica Colonization in Wheat Roots
2
Characterization of MWCNT/WPU Coating
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The morphology of the MWCNT in the uncured MWCNT/WPU coating that was just brushed or sprayed on the surface of the steel substrate was observed using a JEM-3010 high-resolution transmission electron microscope (TEM) (JEOL, Tokyo, Japan) to characterize the dispersion of MWCNTs in the WPU resins. The cross-sectional morphology of the coating was investigated using a Merlin Compact scanning electron microscope (SEM) (Zeiss, Oberkochen, Germany) to characterize the dispersion of MWCNTs in the coating.
When the test on the wear was finished, the surface morphology of the wear track was observed with a VEGA3 XMU SEM (TESCANSCAN, Brno, Czech) to characterize the effect of ES on the wear resistance of the water-based conductive coating.
When the test on the wear was finished, the surface morphology of the wear track was observed with a VEGA3 XMU SEM (TESCANSCAN, Brno, Czech) to characterize the effect of ES on the wear resistance of the water-based conductive coating.
3
Characterization of CuO Nanostructures
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The electrochemistry tests were conducted at a CHI 760E workstation (ChenHua, China). A Talos F200S transmission electron microscope (FEI, USA) and a Merlin Compact scanning electron microscope (Zeiss, Germany) provided the morphology results. Samples for transmission electron microscopy (TEM) were prepared by placing a drop of precipitate (dispersed in water by ultrasonication for 10 min) on a carbon-coated copper grid and allowing it to dry in air. The X-ray diffraction (XRD) measurements were implemented with a D8 Advance diffractometer (Bruker, Germany). The Raman spectra of the CuO nanostructures were recorded on a LabRAM HR Evolution spectrophotometer equipped with an Ar ion 514 nm laser (Horiba, Japan). The X-ray photoelectron spectra (XPS) were recorded on a K-Alpha spectrometer (Thermo, USA). The absorption spectral study was performed using a 1800PC UV-Vis spectroscope (Mapada, China) with a deuterium lamp as the irradiation source and phosphate-buffered saline (PBS) as the reference. A ZEN3690 Zetasizer (Malvern, UK) was applied to measure the zeta potential and size distribution, and the test temperature was 25 °C, the number of cycles was 10, and the dispersant was water.
4
Microstructure Analysis of HPIDF/F-HPIDF
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Merlin Compact scanning electron microscope (SEM; Carl Zeiss Jena GmbH, Jena, Germany) was used to observed the microstructure of HPIDF/F-HPIDF after the spray gold treatment. The scanning images were captured at accelerating voltages of 5.00 kV. All images were recorded at magnifications of 300× (low magnification) and 3000× (high magnification).
5
Morphological and Crystalline Analysis of Ag Powders and CNT/Ag/AgCl
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The morphologies and crystalline phases of the as-obtained Ag powders and CNT/Ag/AgCl-721 were investigated by scanning electron microscopy (SEM) and powder X-ray diffraction (XRD). The SEM images were collected from a Merlin Compact scanning electron microscope (Carl ZEISS, Jena, Germany). XRD measurements were carried out by an XRD-6000 diffractometer (Shimadzu, Tokyo, Japan) operating at 30 kV and 30 mA with Cu Kα radiation (λ = 1.54056° A). Scans were typically carried out from 10 to 90° with a scan rate of 5° min−1.
6
Physicochemical Characterization of CuCo-PTC MOF
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Fluorescence emission spectra were recorded with a Perkin Elmer uorescence spectrophotometer (Waltham, Massachusetts, USA). The appearance of CuCo-MOF was characterised by the Zeiss MERLIN Compact scanning electron microscope. The size and morphology of CuCo-PTC MOF were studied on a JEOL JEM 2100 transmission electron microscope. X-ray photoelectron spectroscopy was performed with a Thermo Scienti c K-Alpha X-ray diffraction spectroscope. The structural changes were observed with a Bruker D8 Advance X-Ray powder diffractometer. The infrared spectra were recorded on a Thermo Scienti c Nicolet iS10 Fourier infrared spectroscope. Thermogravimetric analysis was performed on a TG 209F1 thermogravimetric analyser. The physical adsorption and desorption of nitrogen were studied with a MacTriStar II 3FlexTriStar II 3Flex speci c surface and porosity analyser.
7
Comprehensive Characterization of Nanostructured Materials
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The crystalline structure of the sample was characterized using a Bruker D8 FOCUS X-ray powder diffractometer (XRD, Cu Kα radiation, Bruker Corporation, Billerica, MA, USA). The size, shape, and nanostructure were investigated on a ZEISS MERLIN Compact scanning electron microscope (SEM, Carl Zeiss AG, Oberkochen, Germany) and a Tecnai G2 F20 transmission electron microscope (TEM, Frequency Electronics Inc., Hillsboro, OH, USA). X-ray photoelectron spectra (XPS) were obtained using a Thermo ESCALAB 250XI electron spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Raman spectrum was taken by a Renishaw inVia Raman microscope (Renishaw Company, Gloucestershire, UK). Nitrogen adsorption/desorption test was performed on an ASAP 2020/Tristar 3000 instrument (Micromeritics Instrument Corporation, Norcross, GA, USA). The amount of carbon materials in the sample was assessed using SDT Q600 thermal gravimetric analysis (TG, TA Instruments, New Castle, DE, USA).
8
Morphometric Analysis of Drosophila Sex Combs
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Image acquisition and observation of secondary sexual traits were performed using a SteREO Discovery V12 (Zeiss) and a ZEISS Merlin Compact Scanning Electron Microscope. Image analysis and measurements of the different body parts were performed using Zen software (Zeiss) and Fiji software [21 (link)]. For measurements of the teeth structures composing the sex combs, we measured the length of 20 teeth in three different males for both yfp control and Scr RNAi, which corresponds to an effective yfp control n = 60, Scr RNAi n = 60. Measurements of pronotum between control and Scr RNAi have been performed on: control n = 12 (4 yfp + 8 wild-type), Scr RNAi n = 7. Then measurements and quantification of Ubx RNAi phenotypes have been performed with: yfp control males n = 21, Ubx RNAi males n = 9, yfp control females n = 12, Ubx RNAi females n = 10.
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