To assess the cytotoxicity of HFS associated with the MSCs we used TEM. The HFS and HFS + MSCs were fixed for 3 hours with Karnovsky’s fixative (1% paraformaldehyde, 2.5% glutaraldehyde in 0.1 M phosphate-buffered saline; PBS, pH 7.3). After fixation, the samples were washed with 0.1 PBS, pH 7.3 (3 × 5-minute washes), and then post-fixed in 1% osmium tetroxide diluted in the same buffer for 2 hours. The samples were then washed with distilled water (3 × 10-minute washes), immersed in uranyl acetate (0.5% in distilled water) for 2 hours, dehydrated in increasing concentrations of acetone, and immersed in 100% acetone/Araldite® resin (1:1) for 12 hours. Ultra-thin 90 nm sections were prepared and stained with uranyl acetate (50% in alcohol) for 20 minutes. Subsequently, the sections were stained with lead citrate for 10 minutes. The samples were then analyzed by TEM with an FEI Tecnai Spirit system (Hillsboro, OR, USA).
Tecnai spirit system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Tecnai Spirit system is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a robust and reliable design, advanced optics, and user-friendly software to enable efficient sample preparation, imaging, and data acquisition.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using tecnai spirit system
1
TEM Analysis of MSC Cytotoxicity
2
Labeling Mesenchymal Stem Cells with Iron Oxide Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Iron oxide nanoparticles (100 µg/mL) were detected using a Hitachi H-800 electron microscope (Hitachi Ltd., Tokyo, Japan) at an acceleration voltage of 200 kV with a charge-coupled device camera. MSCs were labeled with Fe3O4@PDA or Fe3O4 (50 µg/mL) for 16 hours and washed twice with PBS. MSCs were then fixed in phosphate-buffered Karnofsky’s solution, followed by staining with 2% osmium tetroxide at 4°C overnight, dehydration using graded ethanol, and embedding in Epon 812 resin. Resin blocks were sectioned using a microtome, doubly stained with uranyl acetate and lead hydroxide, and imaged using a Tecnai Spirit system (120 kV; FEI, Hillsboro, OR, USA).
3
TEM Imaging of Fe3O4 Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The morphological structure of Fe3O4 NPs (100 μg/mL) was examined using the Hitachi H-800 Electron Microscope (Hitachi, Tokyo, Japan) equipped with a charge-coupled device camera operating at an acceleration voltage of 200 kV. For TEM, the cells were fixed in phosphate-buffered Karnofsky’s fixative solution, stained with 2% osmium tetroxide over night at 4°C, dehydrated, embedded in epoxy resin, and cut into 70-nm sections. The sections were collected on copper grids containing Formvar support film. The samples were analyzed using the Tecnai Spirit system (120 kV; FEI, Hillsboro, OR, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!