The brains were quickly removed, and several pieces of 1 mm3 peri-ischemic cortex tissues were dissected and fixed in 2.5% glutaraldehyde in 0.2 mol/L PBS for at least 2 h at 4 °C. The samples were dehydrated with a graded series of ethanol and acetone and then embedded and cured in epoxy resin. Ultrathin sections were cut using an ultramicrotome and then double-stained with uranyl acetate and lead citrate. The ultrastructure of mitochondrial morphology was observed using a TEM-1400 Plus electron microscope (JEOL JEM-1400 Plus, Tokyo, Japan).
Tem 1400 plus electron
Manufactured by JEOL
Sourced in Japan
The TEM-1400 Plus is a transmission electron microscope (TEM) designed and manufactured by JEOL. It is a versatile instrument that enables high-resolution imaging of samples at the nanoscale level. The TEM-1400 Plus provides users with the ability to observe the detailed structure and composition of materials, making it a valuable tool for a wide range of scientific and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tem 1400 plus electron
1
Ultrastructural Analysis of Mitochondrial Morphology
2
Transmission Electron Microscopy of Colon
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TEM was conducted based on previously described approaches 38 . Briefly, colonic segments were cut into 0.1 cm × 0.1 cm × 0.1 cm samples and instantly fixed with 2.5% glutaraldehyde at 4 °C for 4 h. Then, the samples were then dehydrated in ethanol and propylene oxide and embedded in epoxy resin for cutting into ultrathin sections. The sections were stained with uranyl acetate and lead citrate, followed by photography under a TEM-1400 Plus electron microscope (JEOL JEM-1400 Plus, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!