Petroleum ether

Manufactured by Loba Chemie

Sourced in India

Petroleum ether is a colorless, volatile, and flammable liquid solvent. It is primarily used in laboratories as a general-purpose extractant and solvent for organic compounds.

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Lab products found in correlation

7 protocols using petroleum ether

Solvent-Assisted Synthesis of Biomass-Derived Compounds

Cited 1 time

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Petroleum ether (60–80 °C, 99%), ethyl acetate (99%), and methanol (99%) were purchased from Loba Chemie Pvt. Ltd. Benzene (99%), toluene (99%), o-xylene (99%), m-xylene (99%), p-xylene (99%), isopropylbenzene (99%), ethylbenzene (99%), mesitylene (99%), zinc chloride (anhydrous, 95%), nitromethane (99%), molecular sieve (4 Å), and silica gel (60–120 mesh) were procured from Spectrochem. Palladium(ii) acetate (98%), 10% Pd/C, and Amberlyst-15 were procured from Sigma-Aldrich. 5-(Acetoxymethyl)furfural (AcMF) was prepared from fructose following a literature process.^{28 (link)} The solvents were dried over activated molecular sieves for 24 h before use. All the chemicals were used as received without further purification.

Yadav A.K., Bhat N.S., Kaushik S., Seikh A.H, & Dutta S. (2024). Efficient preparation of hybrid biofuels from biomass-derived 5-(acetoxymethyl)furfural and petroleum-derived aromatic hydrocarbons. RSC Advances, 14(5), 3096-3103.

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Extraction of Phytochemicals and Nephroprotection

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Chemicals for extraction of phytochemical constituents such as petroleum ether (98%) and ethanol (99%) were used from LOBACHEMIE, Ltd. (India). Chemicals such as ketamine (100 mg/kg) and xylazine (12.5 mg/kg) were used for anesthesia. Gentamycin and silymarin (from Jimma University pharmaceutical laboratory) were used as negative and positive controls during the nephroprotective study, respectively. All the chemicals and reagents used in this study were analytical grade.

Meka Kedir W., Dukassa Dubiwak A, & Tofik Ahmed E. (2022). Nephroprotective Effect of Asparagus africanus Lam. Root Extract against Gentamicin-Induced Nephrotoxicity in Swiss Albino Mice. Journal of Toxicology, 2022, 8440019.

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Diesel-based Biodiesel Synthesis

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Diesel was procured from an outlet of Indian Oil Corporation Limited (IOCL). Silica gel (60–120 mesh) and petroleum ether (60–80 °C) were purchased from Loba Chemie Private Limited. Ethyl levulinate (98%) was purchased from Sigma Aldrich.

Mohan A., Dutta S., Balusamy S, & Madav V. (2021). Liquid fuel from waste tires: novel refining, advanced characterization and utilization in engines with ethyl levulinate as an additive. RSC Advances, 11(17), 9807-9826.

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Synthesis of Quinoline and Acridine Derivatives

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4,7-Dichloroquinoline 97%, ethylenediamine 99%, hydrazine hydrate 98%, 4,4'-methylenedianiline 97%, triethylamine 99%, coumarin-3-carboxylic acid 99%, were purchased from Sigma Aldrich, MO, USA. Also, reagents as 1,3-Diaminopropane 99%, 1,4-Phenylenediamine, thionyl chloride 97% and solvents as ethanol 98%, dichloromethane 98%, and petroleum ether with 60–80 °C, were supplied from LOBA Chemie, Mumbai, India and used without any further purification. The prepared starting materials such as 9-chloroacridne and 11-chloroneocryptolepine and their corresponding free amines were prepared as reported^{42 (link),43 (link)}.

Abdelaziz E., El-Deeb N.M., Zayed M.F., Hasanein A.M., El Sayed I.E., Elmongy E.I, & Kamoun E.A. (2023). Synthesis and in-vitro anti-proliferative with antimicrobial activity of new coumarin containing heterocycles hybrids. Scientific Reports, 13, 22791.

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Phytochemical Extraction and Purification

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Analytical-grade chloroform, methanol, petroleum ether, and ethyl acetate (Loba Chemie Pvt. Ltd., Mumbai, India) were used for extraction and column elution. A rotary evaporator (Labo Rota 4000, Heidolph Instrument) was used for extract concentration. Column chromatographic separation was done on silica gel 60–120 mm (Merck, Darmstadt, Germany). Further purification was carried out on Sephadex LH-20. TLC analysis was performed on pre-coated silica gel 60 F₂₅₄ plates (Merck, Darmstadt, Germany) using petroleum ether and ethyl acetate as mobile phase. Visualization of spots on the TLC plate was achieved by using UV lamps (UV-Tec 254 nm and 365 nm) and iodine vapor. ¹H NMR (400 MHz and 500 MHz) and ¹³C NMR (100 MHz and 125 MHz) spectra were recorded on the Bruker Avance 400 and 500 Spectrometers, respectively, using CDCl₃ as a solvent. Distilled water, Mueller-Hinton agar, nutrient broth, DMSO, gentamicin, and clotrimazole were used for antibacterial and antifungal activity tests.

Deresa E.M., Abdissa N, & Abdissa D. (2024). Chemical constituents of the roots of Kniphofia schimperi and evaluation of their antimicrobial activity. Heliyon, 10(7), e28927.

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Antioxidant Activity Determination

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All chemicals and reagents used were of reagent grades. Solvents such as petroleum ether, chloroform, and methanol were obtained from Loba Chemie Pvt, Ltd (Mumbai, India). The 2,2-diphenyl-1-picrylhydrazyl (DPPH, 85%) and Folin-Ciocalteu reagent (2 N) were supplied by Alpha Chemika (Mumbai, India) and Sisco Research Laboratories Pvt. Ltd. (Navi Mumbai, Maharashtra, India), respectively. Sodium carbonate anhydrous (99.5%) was purchased from Blulux Laboratory Pvt (Faridabad, Haryana, India). l-Ascorbic acid (99%) and gallic acid (99%) were purchased from Nice Chemicals Pvt (Ernakulum, Kerala, India).

Molole G.J., Gure A, & Abdissa N. (2022). Determination of total phenolic content and antioxidant activity of Commiphora mollis (Oliv.) Engl. resin. BMC Chemistry, 16(1), 48.

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Phytochemical Analysis and Antioxidant Evaluation

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All the chemicals were of good grade and were purchased
from HiMedia
Leading BioScience Company. The chemicals and reagents, which are
used during the project work are as follows: sodium hydroxide, sodium
chloride, sodium acetate, methanol, petroleum ether, Folin–Ciocalteu
reagent, sodium hydroxide, sodium carbonate, sodium nitrite, and aluminum
chloride, which were obtained from Loba Chemie (Mumbai, India). Gallic
acid, quercetin, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained
from Sigma-Aldrich (St. Louis, Missouri, USA).

S.a.n.y.u.k.t.a., Brar D.S., Pant K., Kaur S., Nanda V., Nayik G.A., Ramniwas S., Rasane P, & Ercisli S. (2023). Comprehensive Analysis of Physicochemical, Functional, Thermal, and Morphological Properties of Microgreens from Different Botanical Sources. ACS Omega, 8(32), 29558-29567.

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