Acrylamide solution

Manufactured by Merck Group

Sourced in United States

Acrylamide solution is a clear, viscous liquid used in various laboratory applications. It serves as a key component in the preparation of polyacrylamide gels, which are widely utilized in analytical techniques such as electrophoresis. The solution provides a consistent and reliable source of acrylamide, a chemical essential for the formation of these gels.

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Acrylamide (504 citations) Acrylamide/bis-acrylamide 30% solution (5 citations) Acrylamide/bis-acrylamide solution (4 citations) 30% acrylamide/bis solution (4 citations) Acrylamide-d3 solution (3 citations) Acrylamide-d3 standard solution (2 citations) 30% solution of acyrlamide/bis-acrylamide (2 citations)

7 protocols using acrylamide solution

Acrydite-Modified Nucleic Acid Strand Synthesis

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4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), ammonium persulfate (APS), N,N,N′,N′-tetramethyl-ethylenediamine (TEMED), acrylamide solution (40%), hemin, potassium chloride, kryptofix [2.2.2], aniline and hydrogen peroxide were purchased from Sigma-Aldrich. Desalted 5′ end acrydite-modified nucleic acid strand (1) (acrydite-AAGGGTTAGGG) was purchased from Integrated DNA Technologies Inc. (Coralville, IA). Ultrapure water purified by a NANOpure Diamond instrument (Barnstead International, Dubuque, IA, USA) was used to prepare all of the solutions.

Lu C.H., Guo W., Qi X.J., Neubauer A., Paltiel Y, & Willner I. (2015). Hemin–G-quadruplex-crosslinked poly-N-isopropylacrylamide hydrogel: a catalytic matrix for the deposition of conductive polyaniline †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc02203g. Chemical Science, 6(11), 6659-6664.

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Polyacrylamide Hydrogel Fabrication for MSC Culture

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25-mm circular glass coverslips were treated first with ethanol, then RCA solution (1:1:3 for 15N NH₄OH:30% H₂O₂:dH₂O), and functionalized with 1% allyltrichlorosilane and 1% triethylamine in chloroform solution. N,N′methylene-bis-acrylamide (Sigma) and the acrylamide solution (40%, Sigma) was mixed at the final concentrations in PBS. For 1 kPa gels, 3% acrylamide and 0.11% bisacrylamide was used and for 34 kPa, % acrylamide and 0.3% bisacrylamide was used. Solution was polymerized on a coverslip with 0.1% ammonium persulfate and 0.1% N,N,N′,N′-tetramethylethylenediamine. During polymerization, gels were covered with another coverslip that was pretreated with dichlorodimethylsilane. Sulfo-Sanpah (Pierce) was diluted in HEPES buffer pH 8.0 to 0.5 mg/mL and 300 uL was added to the gel and then reacted using 365 nm UV light for 10 min. Excess Sulfo-Sanpah was removed by 2 washes of buffer. Type I rat-tail collagen was diluted to 0.2 mg/mL in HEPES buffer pH 8.0 and incubated with the gel at 4°C for 4 hours. Collagen was removed and the gel was equilibrated with PBS. MSCs were plated onto gels within 24 hours of collagen attachment.

Raab M, & Discher D.E. (2017). Matrix rigidity regulates the microtubule network polarization in migration. Cytoskeleton (Hoboken, N.J.), 74(3), 114-124.

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Fabrication of Heat-Responsive Egg White Phantom

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Based on previously described methods [16 (link)], an egg white-based, heat-responsive phantom was fabricated in the study. It was nearly transparent at room temperature. When heated up to 60°C, the phantom became a visibly opaque lesion because of the denaturation and coagulation of egg white protein.
The phantom consisted of 15% acrylamide solution (Sigma-Aldrich, St. Louis, MO), 40% egg white, 44.5% degassed deionized water, and 0.5% ammonium persulfate solution (Sigma-Aldrich). After the mixed solution was degassed for 10 min, 0.2 mL MSNC-PFH nanoemulsion was added and then stirred gently to achieve a uniform distribution. The concentration of droplets was 10⁷ droplets/mL in the phantom. In comparison, the same amount of phosphate buffered saline (PBS) was added as a control (PBS-control) without MSNC-PFH nanoemulsion. Finally, 0.15 mL 1,2-bis(dimethylamino)ethane (Sigma-Aldrich) was added to the entire solution to initiate polymerization. The phantom was kept in a 12°C water bath during polymerization period. The dimensions of each phantom used in the experiments were around 6 × 5 × 3.3 cm.

Zhao L.Y., Zou J.Z., Chen Z.G., Liu S., Jiao J, & Wu F. (2016). Acoustic Cavitation Enhances Focused Ultrasound Ablation with Phase-Shift Inorganic Perfluorohexane Nanoemulsions: An In Vitro Study Using a Clinical Device. BioMed Research International, 2016, 7936902.

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Synthesis of Silica-Titania Nanocomposites

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Absolute ethanol (ACS reagent), K₂Cr₂O₇ (≥99.0 %), acrylamide solution (40 % in H₂O), tetraethyl orthosilicate (TEOS, 98 %), NH₃ (28 wt %), titanium(IV) butoxide (TBT, 97 %), NaOH (pellets EMPLURA) and HCl (37 wt %) were all purchased from Sigma–Aldrich. All the reagents were used as received. Milli‐Q water (resistivity higher than 18.2 MΩ cm⁻¹) was collected from a Millipore academic purification system.

Tan J.Z., Xia F, & Maroto‐Valer M.M. (2019). Raspberry‐Like Microspheres of Core–Shell Cr2O3@TiO2 Nanoparticles for CO2 Photoreduction. Chemsuschem, 12(24), 5246-5252.

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Preparation of spin-labeled lipids

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Fmoc protected amino acid and coupling reagents were purchased from NovaBiochem and used without further purification. H-PAL Chemmatrix resin was from Sigma Aldrich. Peptide synthesis and purification solvents were from Romil (Biopure grade). Piperidine and N,N’-diisopropyl ethylamine were from NovaBiochem. The fluorescent probe DPH (1,6-Diphenyl-1,3,5-hexatriene) and acrylamide solution (40% w/v) were obtained from Sigma Aldrich Chemical. The lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phospho-1′-rac-glycerol (DPPG) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-1′-rac-glycerol (POPG) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA) and used without further purifications. Spin-labeled phosphatidylcholines (1-palmitoyl-2-stearoyl-(n-doxyl)-sn-glycero-3-phosphocholine, n-PCSL) with the nitroxide group in the positions 5, 14 of the acyl chain for EPR experiments were also obtained from Avanti Polar Lipids. The chemical structures of all the lipids used are shown in Fig. S1. Deionized water was used for the phosphate buffer and all sample preparations.

Oliva R., Chino M., Pane K., Pistorio V., De Santis A., Pizzo E., D’Errico G., Pavone V., Lombardi A., Del Vecchio P., Notomista E., Nastri F, & Petraccone L. (2018). Exploring the role of unnatural amino acids in antimicrobial peptides. Scientific Reports, 8, 8888.

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Microfluidic Synthesis of Lipid Nanoparticles

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Acrylamide solution (AA; 40% (wt/wt)), AA/bis-Acrylamide solution, 40% (wt/wt), molar ratio 19:1 (AA/BIS), N,N,N′,N′-Tetramethylethylenediamine (TEMED), ammonium persulfate (APS), Span-80, polyvinyl alcohol (PVA) (MW 30 000–70 000, 89% hydrolyzed), Poly (L-lactide-co-glycolide) (PLGA) (RG502H, MW 7000–17 000), mineral oil (BioReagent for molecular biology), and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dimethyl carbonate (DMC), glycerol, and hexane were obtained from Acros Organics (Pittsburgh, PA, USA). The 1H,1H,2H,2H-Perfluorooctanol was purchased from Alfa Aesar, Shanghai, China. 3M Novec 7500 Engineered Fluid was purchased from Flurochem, Derbyshire, UK. Fluorosurfactants were obtained from ThunderBio, Hong Kong, China. Lipids of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (DPPG), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(poly(ethylene glycol))- 2000] (DSPE-PEG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). All aqueous solutions were prepared in Milli-Q deionized water (18 MΩ/cm, Millipore CO., Burlington, MA, USA).

Chung C.H., Cui B., Song R., Liu X., Xu X, & Yao S. (2019). Scalable Production of Monodisperse Functional Microspheres by Multilayer Parallelization of High Aspect Ratio Microfluidic Channels. Micromachines, 10(9), 592.

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HPLC Analysis of Acrylamide

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Acrylamide was analyzed in triplicate following the method by Galani et al. (2017) with slight modifications. A HPLC system (Agilent 1200 series, Santa Clara, CA, USA) equipped with an auto sampler and a diode array detector was used. Two microliters of sample were injected by auto sampler and separated using a Zorbax 300 Extend C18 analytical column (2.1 mm x 100 mm, 3.5 µm) (Santa Clara, California, USA). Formic acid (Sigma-Aldrich, F0507) (0.1% in water) was used as mobile phase with flow rate of 0.1 mL/min. The running time was 5 min and retention time of acrylamide was at 3.4 min. Acrylamide solution (Sigma-Aldrich, A9099) (1 mg/mL) was prepared, then a series of acrylamide concentrations (1 to 100 g/mL) were prepared from the stock solution. The position of the acrylamide peak in reaction mixtures was confirmed by spiking. All test samples were diluted in Milli-Q water to be within the standard range and filtered with Nylon filter (0.45 µm) (Sigma-Aldrich Co., St. Louis, MO, USA) and transferred to a vial for chromatography analysis.

Champrasert O., Chu J., Meng Q., Viney S., Holmes M., Suwannaporn P, & Orfila C. (2021). Inhibitory effect of polysaccharides on acrylamide formation in chemical and food model systems. Food chemistry, 363.

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