Rabbit polyclonal anti-TSG101 (14497-1-AP; 1:1000), rabbit polyclonal anti-HSP70 (10995-1-AP; 1:1000), and rabbit polyclonal anti-MITF (Proteintech1092-1-AP; 1:1000) were obtained from Proteintech (China). Rabbit polyclonal anti-CD63 (ab118307; 1:1000) and rabbit polyclonal anti-Tyrosinase (ab170905; 1:1000) were procured from Abcam (England). Rabbit polyclonal anti-GAPDH (SB100242-T40; 1:1000) and rabbit polyclonal anti-Calnexin (CST2679; 1:1000) were obtained from Sino Biological (China) and CST (USA), respectively. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (A#21020; 1:5000) and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (A#21010; 1:5000) were obtained from Abbkine (USA). Mouse polyclonal anti-CD9 (1:1000) antibody was donated by Yuan Gao.
Anti tsg101 14497 1 ap
Manufactured by Proteintech
Sourced in China, United States, United Kingdom
Anti-TSG101 (14497-1-AP) is a primary antibody that specifically targets the TSG101 (Tumor Susceptibility Gene 101) protein. TSG101 is a key component of the ESCRT (Endosomal Sorting Complexes Required for Transport) machinery, which is involved in various cellular processes such as endosome trafficking and the formation of multivesicular bodies. This antibody can be used for applications such as Western blotting, immunohistochemistry, and immunoprecipitation to detect and study the TSG101 protein.
Automatically generated - may contain errors
3 protocols using anti tsg101 14497 1 ap
1
Comprehensive Antibody Panel Profiling
2
Exosomal Surface Marker Analysis
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To measure exosomal surface markers, western blot analysis was conducted. Briefly, the exosome supernatant was denatured in sodium dodecyl sulfonate (SDS) buffer and then incubated with anti-CD81 (66866-1-Ig; Proteintech, USA), anti-TSG101 (14497-1-AP; Proteintech), and anti-HSP70 (66183-1-Ig; Proteintech).
3
Immunoblot Analysis of LRSAM1, TSG101, and GAPDH
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Immunoblot was executed as reported. 14 Anti-LRSAM1 (#ab73113, Abcam, Cambridge, UK), anti-TSG101 (#14497-1-AP, Proteintech, Rosemont, IL) and anti-GAPDH (#GTX100118, Genetex, Irvine, CA) antibodies were used. Results were visualized on the ImageQuant TM LAS4000 (GE Healthcare Life Sciences).
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