Rodent diet d12492

Manufactured by Research Diets

Sourced in United States

Rodent Diet D12492 is a semi-purified, high-fat diet formulated for nutritional studies in rodents. It provides 60% of calories from fat. The diet is designed to induce obesity and metabolic disturbances in animal models.

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Lab products found in correlation

C57bl 6j mice, by Central Lab Animal (1 mentions) C57bl 6j male mice, by Jackson ImmunoResearch (1 mentions) Legend micro 17r, by Thermo Fisher Scientific (1 mentions) D12492, by Research Diets (1 mentions) Zoletil, by Virbac (1 mentions)

4 protocols using rodent diet d12492

Dietary Influence on Murine Metabolism

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Six-week-old male C57BL/6J mice (20–22 g) were supplied by Central Lab. Animal Inc., Seoul, Korea. The animals were housed and maintained in a standard laboratory condition (room temperature: 24 ± 1°C, relative humidity: 50–60%, light cycle: 7:00–19:00). The animal study was approved by the Animal Experiment Ethical Committee of the Wonkwang University (Approval number: WKU19-76). The mice were acclimatized to their environment for 1 week before the commencement of the experiments.
Mice were fed with either a standard chow diet (Standard diet: 5L79 Orient Bio Inc., Seongnam, Korea) consisting of 13.67% fat; 65.30% carbohydrate; and 20.1% protein or a high-fat diet (Rodent Diet D12492, Research Diets, New Brunswick, NJ, United States) consisting of 60% fat; 20% carbohydrate; and 20% protein. The compositions of experimental diets used in this study are presented in Supplementary Table S6. Mice were allowed free access to feed and water.

Pandeya P.R., Lamichhane R., Lamichhane G., Lee K.H., Lee H.K., Rhee S.J, & Jung H.J. (2021). 18KHT01, a Potent Anti-Obesity Polyherbal Formulation. Frontiers in Pharmacology, 12, 807081.

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Metabolic Dysregulation in Rat Models

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Male Sprague Dawley rats (250–265 g, Charles River (UPenn) or Envigo (PBRC)) were maintained on either standard chow (Purina Rodent Chow 5001; Ralston Purina Company, St. Louis, Missouri) or a 60% high-fat diet (HFD; Rodent Diet D12492; Research Diets, New Brunswick, New Jersey) with ad libitum access to tap water. Zucker diabetic fatty rats (ZDF; Charles River) were maintained on a standard chow diet. Animals were single-housed in hanging wire cages in a temperature- and humidity-controlled environment on a reverse 12h:12h light-dark cycle. All behavioral experiments were carried out in a counter-balanced, within-subjects design, no randomization required. All experimental procedures were conducted with the approval of the University of Pennsylvania and Pennington Biomedical Research Center Institutional Animal Care and Use Committees.

Stein L.M., Lhamo R., Cao A., Workinger J., Tinsley I., Doyle R.P., Grill H.J., Hermann G.E., Rogers R.C, & Hayes M.R. (2020). Dorsal vagal complex and hypothalamic glia differentially respond to leptin and energy balance dysregulation. Translational Psychiatry, 10, 90.

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Dietary Effects on Mouse Metabolism

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Standard diet (SD) and high-fat diet (HFD) fed C57BL/6J male mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Only male mice were included in this study as gender is a documented factor that influences the expression and activity of some P450^{24 (link)}, and we chose to constrain variables to ensure a focused study scope. The mice were acclimated to the AALAS-accredited animal facility as well as to the nose-only restraint tubes for one week prior to the initiation of CSE. Feed and tap water were provided ad libitum, except when animals were placed in exposure tubes. All animals were observed twice daily for mortality and moribundity. All procedures were carried out with the approval of the Institutional Animal Care and Use Committees at Pacific Northwest National Laboratory. The SD diets consisted of PMI® 5002 Certified Rodent Diet (PMI 5002 Rodent Diet®, Richmond, IN; ~13 kcal% fat) HFD were D12492 Rodent Diet (Research Diets Inc., New Brunswick, NJ; 60 kcal% fat) starting at six weeks of age and continued throughout the study. The HFD mice were fed this diet for nine weeks prior to the start of the two-week CSE regime resulting in a significant increase in body weight compared to SD animals over the same time period (Figure 2).

Sadler N.C., Webb Robertson B.J., Clauss T.R., Pounds J.G., Corley R, & Wright A.T. (2018). High-fat diets alter the modulatory effects of xenobiotics on cytochrome P450 activities. Chemical research in toxicology, 31(5), 308-318.

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Obesity Induction in C57BL/6 Mice

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The experiments were approved by the Animal Ethics Committee of the Dongshin University (Approval No. 2019-07-02). To evaluate the effects of obesity, C57BL/6 mice were purchased from SamTako BioKorea (Osan, Korea) and acclimatized for 7 days. According to the type of diet, 12 mice were divided into two groups (Research Diet, Inc. Product #D12492, New Brunswick, NJ, USA) and given tap water for 8 weeks: (1) control group (n = 6); (2) obesity group with a HFD (D12492 Rodent Diet with 60% fat, 20% protein, 20% carbohydrate, Research Diets, Inc., New Brunswick, NY, USA) (n = 6). Ingredients used for the control and obesity group diets are provided in Tables S5 and S6.
The body weights of the mice were recorded once per week. All mice were anesthetized with 50 mg/kg Zoletil (Virbac, TX, USA). To determine metabolites in the serum, the collected whole blood was centrifuged at 12,000 rpm using a Legend micro 17-R (Thermo Fisher Scientific, Waltham, MA, USA). TCHO-P III and GLU-P III levels were measured. Mice were euthanized using Zoletil over-dosing, then the abdominal fat tissues were collected and the weights of these tissues were measured.

Jo J.K., Seo S.H., Park S.E., Kim H.W., Kim E.J., Kim J.S., Pyo J.Y., Cho K.M., Kwon S.J., Park D.H, & Son H.S. (2021). Gut Microbiome and Metabolome Profiles Associated with High-Fat Diet in Mice. Metabolites, 11(8), 482.

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