Nap 10 sephadex g 25 column
The NAP-10 Sephadex G-25 column is a disposable, pre-packed column used for desalting and buffer exchange of small molecules, proteins, and peptides. The column is filled with Sephadex G-25 resin, which provides efficient separation and removal of salts, buffers, and other low molecular weight compounds from sample solutions.
6 protocols using nap 10 sephadex g 25 column
Calcein Leakage Assay for Lipid Vesicles
Conjugation of scFvs to Tetrazine-Peptide
Example 25
This example aimed to demonstrate that three scFvs could be conjugated to the three PEG12-maleimide linking arms based on tetrazine-peptide 2. Prior to conjugation with the tetrazine-peptide 2 that had three PEG12-maleimide linking arms, 1F10 scFv was incubated with DTT at a molar ratio of 2:1 ([DTT]:[scFv]) at 25° C. for 4 hours with gentle shaking to keep its C-terminal cysteine in reduced form. Subsequently, the buffer of reduced 1F10 scFv was exchanged to maleimide-SH coupling reaction buffer (100 mM sodium phosphate, pH 7.0, 50 mM NaCl and 5 mM EDTA) by using an NAP-10 Sephadex G-25 column (GE Healthcare). After the reduction and buffer exchange, the conjugation to the tetrazine-peptide 2 having three PEG12-maleimide linking arms was conducted overnight at 4° C. at a molar ratio of 1:4 ([linker]:[Protein]).
Conjugation of scFv to Tetrazine-Peptide
Example 26
This example aimed to demonstrate that three scFvs could be conjugated to the three PEG12-maleimide linking arms based on tetrazine-peptide 2. Prior to conjugation with the tetrazine-peptide 2 that had three PEG12-maleimide linking arms, 1F10 scFv was incubated with DTT at a molar ratio of 2:1 ([DTT]:[scFv]) at 25° C. for 4 hours with gentle shaking to keep its C-terminal cysteine in reduced form. Subsequently, the buffer of reduced 1F10 scFv was exchanged to maleimide-SH coupling reaction buffer (100 mM sodium phosphate, pH 7.0, 50 mM NaCl and 5 mM EDTA) by using an NAP-10 Sephadex G-25 column (GE Healthcare). After the reduction and buffer exchange, the conjugation to the tetrazine-peptide 2 having three PEG12-maleimide linking arms was conducted overnight at 4° C. at a molar ratio of 1:4 ([linker]:[Protein]).
Calcein Leakage Assay for Peptide-Lipid Interactions
Calcein Leakage Assay for Liposome Permeabilization
Functionalization of MWCNT with CS-ODN
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