Total RNA was extracted from the skeletal muscle specimens (approximately 50 mg each) using a reagent (RNAiso Plus 9108; Takara Bio, Shiga, Japan), following the manufacturer’s instructions. The concentration (ng/ml) and purity of the freshly extracted total RNA were measured using a microplate reader (Infinite M200 Pro; TECAN, Zurich, Switzerland), and the integrity of total RNA was determined with the 2100 Bioanalyzer and RNA 6000 Nano assay kit (Agilent Technologies, Palo Alto, CA, U.S.A.). Only RNA with an OD260:OD280 ratio in the range 1.8–2.2 and RNA integrity > 7.0 were used in the microarray analysis. Total RNA was used to synthesize sense-strand cDNA (sscDNA) using the WT Expression Kit (Ambion, Austin, TX, U.S.A.). Then, the sscDNA samples were fragmentated and labeled using another kit (GeneChip® WT Terminal Labeling Kit; Affymetrix®, Santa Clara, CA, U.S.A.) and hybridized to array plates (Gene 2.0 ST Arrays; Affymetrix®). Finally, the arrays were scanned using the GeneChip™ Scanner 3000 7G instrument (Thermo Fisher Scientific, Santa Clara, CA, U.S.A.).
Gene 2.0 st array
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Gene 2.0 ST Array is a high-density microarray designed for gene expression analysis. It provides comprehensive coverage of the human transcriptome, allowing for the measurement of expression levels of both coding and non-coding genes.
Automatically generated - may contain errors
Lab products found in correlation
Rna 6000 nano assay, by Agilent Technologies (2 mentions)
2100 bioanalyzer, by Agilent Technologies (2 mentions)
Wt expression kit, by Thermo Fisher Scientific (2 mentions)
Genechip wt terminal labeling kit, by Thermo Fisher Scientific (2 mentions)
Rnaiso plus 9108, by Takara Bio (2 mentions)
Infinite m200 pro, by Tecan (1 mentions)
In nite m200 pro, by Tecan (1 mentions)
Expression console software, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Genechip human gene 2.0 st array, by Thermo Fisher Scientific (1 mentions)
Genechip operating software, by Thermo Fisher Scientific (1 mentions)
Exon 1.0 st array, by Thermo Fisher Scientific (1 mentions)
5 protocols using gene 2.0 st array
1
Transcriptome Analysis of Skeletal Muscle
2
Skeletal Muscle RNA Extraction and Microarray
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Total RNA was extracted from the skeletal muscle specimens (approximately 50 mg each) using a reagent (RNAiso Plus 9108; Takara Bio, Shiga, Japan), following the manufacturer's instructions. The concentration (ng/mL) and purity of the freshly extracted total RNA were measured using a microplate reader (In nite M200 Pro; TECAN, Zurich, Switzerland), and the integrity of total RNA was determined with the 2100 Bioanalyzer and RNA 6000 Nano assay kit (Agilent Technologies, Palo Alto, CA, USA). Only RNA with an OD260:OD280 ratio in the range 1.8 - 2.2 and RNA integrity > 7.0 were used in the microarray analysis. Total RNA was used to synthesize sense-strand cDNA (sscDNA) using the WT Expression Kit (Ambion, Austin, TX, USA). Then, the sense-strand cDNA samples were fragmentated and labeled using another kit (GeneChip® WT Terminal Labeling Kit; Affymetrix®, Santa Clara, CA, USA) and hybridized to array plates (Gene 2.0 ST Arrays; Affymetrix®). Finally, the arrays were scanned using the GeneChip™ Scanner 3000 7G instrument (Thermo Fisher Scienti c, Santa Clara, CA, USA).
3
Profiling mRNA Profiles in Benzene Poisoning
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Study subjects in mRNA microarray are mainly engaged in painting and control group is the office staff of the same unit. Chronic benzene poisoning was diagnosed according to diagnostic criteria and principles of occupational benzene poisoning (GBZ 68–2008). To control the impact of confounding factors, age, gender, medical history, the history of occupation and lifestyle such as smoking and drinking were matched showed in Table S1. And the detailed information was expounded as previously described [8 (link)]. mRNA microarray was performed as previously described [8 (link)]. Total RNA was extracted from human peripheral blood cells using TRIzol reagent (Invitrogen, USA), then purified RNA was amplified and transcribed into cRNA. cDNA was labeled and hybridized to the GeneChip Human Gene 2.0 ST Array (Affymetrix). The experimentations were scrutinized by GeneChip® Command Console® Software (AGCC), meanwhile, the acquired array images were analyzed by Affymetrix GeneChip Operating Software. Affymetrix® Expression Console™ Software were used to control QC analysis of Gene 2.0 ST Array data.
4
LPS-Induced Inflammatory Response in PTP1B Mice
5
Transcriptome Analysis of Sickle Cell Disease
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