For analyzing autophagy, third instar larvae were dissected in ice-cold PBS and stained with the nuclear dye Hoechst 33342 (1:2000) for thirty minutes at room temperature before the fat body was removed and incubated in the dark for two minutes with LysoTracker® (1:100 in PBS) (Invitrogen; Carlsbad CA, USA). The staining solution was removed, and the tissue was mounted in Vectashield (Vector lab; Burlingame CA, USA) and immediately documented with a Zeiss ApoTome using Axio-Imager Software (Carl Zeiss AG; Oberkochen, Germany). For amino acid deprivation, third instar larvae were starved on apple-juice plates [25% apple-juice, 2.4% saccharose, 2.4% agar] without any further food source for 14 hours. The larvae were dissected, and after fixation for 10 minutes in 4% paraformaldehyde the fat body was mounted in glycerol (80%) and directly examined with a Zeiss Axioskop coupled to an ES120 camera (Optronics, Goleta, CA), using Pixera (Santa Clara, CA) Viewfinder software, version 2.0. Pictures were assembled with Corel (Mountain View, CA) PhotoPaint and CorelDRAW software, version 9.0.
Photo paint
Manufactured by Corel
Sourced in Canada
Corel Photo Paint is a powerful image editing software. It provides a comprehensive set of tools for manipulating and enhancing digital images. The software's core function is to enable users to edit, retouch, and composite images.
Automatically generated - may contain errors
Lab products found in correlation
Coreldraw, by Corel (8 mentions)
Nis elements software br 4, by Nikon (4 mentions)
Ds qi1nc, by Nikon (4 mentions)
Coreldraw 11, by Corel (3 mentions)
Eclipse ni microscope, by Nikon (3 mentions)
Ds fi1, by Nikon (2 mentions)
Pds 1000 he system, by Bio-Rad (2 mentions)
Cell d software, by Olympus (2 mentions)
Bx51 epifluorescence microscope, by Olympus (2 mentions)
Dp71 digital camera, by Olympus (2 mentions)
Graph prism 4, by GraphPad (2 mentions)
Zen software, by Zeiss (2 mentions)
Bh 2 microscope, by Leica (2 mentions)
Cellsense standard 1.17 imaging software, by Olympus (2 mentions)
Vectastain abc kit, by Vector Laboratories (2 mentions)
Axioimager software, by Zeiss (1 mentions)
Apotome, by Zeiss (1 mentions)
Es120 camera, by Optronics (1 mentions)
Lysotracker, by Thermo Fisher Scientific (1 mentions)
Vectashield, by Vector Laboratories (1 mentions)
29 protocols using photo paint
1
Autophagy Induction in Drosophila Larvae
2
Microscopic Observation of Coccidian Oöcysts
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Sporulated coccidian oöcysts were observed using an Olympus BX50 microscope. Images were taken using a Nomarski contrast imaging system with a 100× oil immersion objective in combination with an ocular micrometer. All measurements are presented in micrometres with the means in parentheses following the ranges.
Line drawings were edited using two software applications from CorelDRAW® (Corel Draw Graphics Suite, Version 2020, Corel Corporation, Canada), i.e. Corel DRAW and Corel PHOTO-PAINT (Yang et al., 2021 (link)).
Line drawings were edited using two software applications from CorelDRAW® (Corel Draw Graphics Suite, Version 2020, Corel Corporation, Canada), i.e. Corel DRAW and Corel PHOTO-PAINT (Yang et al., 2021 (link)).
3
Microscopic Analysis of Plant Seeds
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Photographs were taken with a Nikon Stereomicroscope Model SMZ1500 equipped with a camera Nikon DS-Fi1 of 5.24 megapixels. The seeds were oriented with the micropyle to the right (Figure 11 ). Composed images containing 40 seeds per accession were prepared with Corel Photo Paint and are stored in: https://zenodo.org/record/4057708#.X3LRpRRxeM8 and https://zenodo.org/record/4035649#.X3LRShRxeM8 .
Confocal images were obtained with a Leica DM IRB TCS SP2 confocal microscope and are limited to a total of 13 species. Each figure contains the mean projection of a series of 20 images.
Confocal images were obtained with a Leica DM IRB TCS SP2 confocal microscope and are limited to a total of 13 species. Each figure contains the mean projection of a series of 20 images.
4
Subcellular Localization of Plant Enzymes
5
Seed Photography Protocol for Taxonomic Identification
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Photographs of the seeds collected in Spain and Portugal were taken in Salamanca with a Nikon Stereomicroscope Model SMZ1500 (Nikon, Tokyo, Japan) equipped with a Nikon DS-Fi1 camera of 5.24 megapixels, and all the other seeds were photographed in Brno with an Olympus SZX9 microscope (Olympus, Japan) coupled to an Olympus OM-D E-M10 II camera. Composed images containing 20 seeds per accession were prepared with Corel Photo Paint for the lateral and dorsal views of the seeds. The images are stored at https://zenodo.org/record/5997053#.YgE1eOrMKM8 (accessed on 15 March 2022).
6
Yeast Phenotype Screening via Drop Dilution
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To examine phenotypes of yeast strains in serial drop dilution assays, cells were grown overnight in selective medium. The cultures were diluted to an OD600 of 0.25 and again grown for 3–5 h. Exponentially growing cells were adjusted to an OD600 of 0.1 with fresh medium and dilutions from 100 to 10−3 were prepared. A quantity of 3 µL of each dilution was spotted onto plates with the indicated compositions and incubated for 2-3 days at 30 °C. Growth was documented by scanning the plates, adjustment of brightness and contrast of the entire images, and trimming using Corel Photo Paint.
7
Genetic Analysis of PKD in Drosophila
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The following fly stocks were used: Oregon R (OreR) and y1w67c23 (BL6599), L1 (BL318), L2 (BL319), L5 (BL321), LG (BL322), PKD26 [40 (link)]; ey-Gal4 [42 (link)], UAS-PKD-WT, UAS-PKD-SE, UAS-PKD-kd [36 (link)]. Further information on fly strains is available at flybase.org . Flies were raised under non-crowded conditions on standard agar-corn-molasses food at 18 °C or 25 °C as indicated. Crosses and combinations were performed with standard genetic techniques; analyses were performed on one to 5 days old flies. Presence of the PKD26 allele in the recombinants was confirmed by PCR using the primer pair: P6 Cre-lox LP, 5′ CCG GAC AGT GGA CTC ACA TA 3′ and P8 white UP, 5′ AAA AGT GCA GCG GAA ATA GTT A 3′ [40 (link)]. Microphotographs of adult heads were taken with a Pixera ES120 digital camera (Optronics) coupled to a Leica M5 using the Pixera Viewfinder Version 2.0 software. Figures were assembled using Corel Photo Paint, Corel Draw, Exel, and BoxPlotR software. Statistical significance of probes was determined by ANOVA two-tailed test for multiple comparisons using Dunnet’s approach with p-values: p > 0.05 (not significant); p < 0.05; p < 0.01; p < 0.001.
8
Seed Image Acquisition and Analysis Protocol
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Photographs were taken with a Nikon Z6 camera with an objective AF-S Micro NIKKOR 60 mm f/2.8G ED. From an initial photograph made to the pool of seeds in each population and containing between 30 and 110 seeds, composed images containing 20–30 seeds regularly oriented per accession were prepared with Corel Photo Paint. In these images, the seeds were aligned to allow further analysis in ImageJ. The images are stored at: https://zenodo.org/record/6205212#.YhOpUujMKM8 (accessed on 21 February 2022).
9
Subcellular Localization of Plant Enzymes
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Determination of the subcellular localization of Rs_SBE and Rt_SBE was performed by subcloning the full length coding sequences into the pSCA-cassette vector as YFP fusions. The nuclear (nucleus-CFP), nucleocytosolic (CFP) and endoplasmic reticulum (ER-CFP) markers were described previously30 ,31 (link). Transient transformation of C. roseus cells by particle bombardment and fluorescence imaging were performed following the procedures previously described30 ,31 (link). C. roseus cells were bombarded with DNA-coated gold particles (1 μm) and 1,100 psi rupture disc at a stopping-screen-to-target distance of 6 cm, using the Bio-Rad PDS1000/He system and 400 ng of each plasmid per transformation. Cells were cultivated for 16 h to 38 h before being harvested and observed. The subcellular localization was determined using an Olympus BX-51 epifluorescence microscope equipped with an Olympus DP-71 digital camera and a combination of YFP and CFP filters. Transformation was repeated three times and for each more than 100 transformed cells were observed. The CellD software (Olympus) was used to control the camera for photos and color attribution. Photos were cropped with Photo-Paint (Corel) to conserve only cells in the figure.
10
Archimedean Spiral Modeling Protocols
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The models were based on the Archimedean spiral with equation, in the following polar coordinates:
where stand for the polar angle and radius, respectively.
Graphic representations of the equations were obtained with Mathematica, and the curves were superimposed in Corel Photo Paint to seed images to verify and quantify the coincidence of shape. Mixed models were composed with a part corresponding to the geometric representation of an algebraic equation and the other obtained from average silhouettes (Figure 1 ).
where stand for the polar angle and radius, respectively.
Graphic representations of the equations were obtained with Mathematica, and the curves were superimposed in Corel Photo Paint to seed images to verify and quantify the coincidence of shape. Mixed models were composed with a part corresponding to the geometric representation of an algebraic equation and the other obtained from average silhouettes (
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