Gapdh mab374 antibody

Manufactured by Merck Group

Sourced in United States

The GAPDH (MAB374) antibody is a monoclonal antibody that recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a key enzyme involved in the glycolytic pathway, catalyzing the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. The GAPDH (MAB374) antibody can be used as a tool for the detection and quantification of GAPDH in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Odyssey imaging system, by LI COR (2 mentions) P nf κb, by Cell Signaling Technology (2 mentions) P jnk, by Cell Signaling Technology (2 mentions) P erk, by Cell Signaling Technology (2 mentions) Hras c 20, by Santa Cruz Biotechnology (1 mentions) Erk1 2 9102, by Cell Signaling Technology (1 mentions) Zeb1 3396, by Cell Signaling Technology (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Bradford method, by Bio-Rad (1 mentions) Vinculin sc 73614, by Santa Cruz Biotechnology (1 mentions) Ab139690, by Abcam (1 mentions) Ab50818, by Abcam (1 mentions) Ab26109, by Abcam (1 mentions) Ab128874, by Abcam (1 mentions) General tissue culture materials, by Avantor (1 mentions) Anti rabbit igg a6667, by Merck Group (1 mentions) I bet151, by Bio-Techne (1 mentions) Sirnas against, by Thermo Fisher Scientific (1 mentions) P21 sc 397, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse, by Merck Group (1 mentions)

8 protocols using gapdh mab374 antibody

Protein Extraction and Immunoblotting Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed and proteins extracted with RIPA buffer [10 (link), 54 (link)]. Protein concentration was measured using the Bradford method (Bio-Rad Laboratories). Samples were separated by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories). The following antibodies were obtained from Santa Cruz Biotechnology Inc.: ADAR1 (SC-73408), p-AKT ser473 (sc-7985-R), AKT (sc-8312), pERK Tyr204 (sc-7383), and HRAS (C-20) (sc-520). ERK1/2 (#9102) and ZEB1 (#3396) antibodies were purchased from Cell Signaling Technology Inc., the PCNA (ab92552) antibody was from Abcam and the GAPDH (MAB374) antibody was from EMD Millipore Corp.

Ramírez-Moya J., Baker A.R., Slack F.J, & Santisteban P. (2020). ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity. Oncogene, 39(18), 3738-3753.

+ Open protocol

+ Expand

Antibody Sources for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA): SP1 (sc-17824), tubulin (sc-5286) and vinculin (sc-73614). CDK13 (ab251955) and Fibrillarin (ab4566) antibodies were purchased from Abcam (Cambridge, UK). The GAPDH (MAB374) antibody was from EMD Millipore Corp. (Billerica, MA) and the hemagglutinin (HA) antibody (C29F4) was from Cell Signaling Technologies, (Danvers, MA).

Ramírez-Moya J., Miliotis C., Baker A.R., Gregory R.I., Slack F.J, & Santisteban P. (2021). An ADAR1-dependent RNA editing event in the cyclin-dependent kinase CDK13 promotes thyroid cancer hallmarks. Molecular Cancer, 20, 115.

+ Open protocol

+ Expand

Chromatin immunoprecipitation protocol for BRD proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

General tissue culture materials were obtained from VWR International. Antibodies against BRD2 (ab139690), BRD3 (ab50818), BRD4 (ab128874) and IRF1 (ab26109) antibody for chromatin immunoprecipitation (ChIP) were obtained from Abcam. Antibodies against c-MYC (5605), human PD-L1 (E1L3N clone; 13684), IRF1 (8478) and control IgG (2729) antibody for ChIP were purchased from Cell Signaling Technology. Anti-BRD4 (A301–985A) antibody for ChIP was obtained from Bethyl Laboratories. PE-conjugated human PD-L1 (MIH1 clone; 12-5983-42) antibody was from Thermo Fisher. The GAPDH (MAB374) antibody was from Millipore, and α-tubulin (sc-8035) antibody and HSP90 (sc-7940) antibody were obtained from Santa Cruz Biotechnology. Secondary anti-mouse IgG (A4416) and anti-rabbit IgG (A6667) antibodies were purchased from Sigma. The BET inhibitors JQ1 and I-BET151 were obtained from Tocris Bioscience, the BET PROTAC ARV-825 was obtained from MedChem Express, and human and mouse IFN-γ was purchased from Thermo Fisher Scientific. The siRNAs against BRD2, BRD3, BRD4, c-MYC, and IRF1 were purchased from Thermo Fisher Scientific.

Ebine K., Kumar K., Pham T.N., Shields M.A., Collier K.A., Shang M., DeCant B.T., Urrutia R., Hwang R.F., Grimaldo S., Principe D.R., Grippo P.J., Bentrem D.J, & Munshi H.G. (2018). Interplay between interferon regulatory factor 1 and BRD4 in the regulation of PD-L1 in pancreatic stellate cells. Scientific Reports, 8, 13225.

+ Open protocol

+ Expand

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells and tissue samples were lysed in RIPA buffer (150 mM NaCl, 50 mM tris-HCl pH8, 1 mM EDTA, 1%NP40, 0.25% sodium deoxycholate, 0.1% SDS), supplemented with protease and phosphatase inhibitor cocktails (Sigma-Aldrich). The determination of protein concentration was measured by colorimetric assay using the pierce BCA protein assay kit.
For the immunoblot analysis, an equal amount of proteins (20–30 µg) was resolved in SDS- polyacrylamide gels (10–12%) and transferred onto PVDF membranes (Amersham). Saturated membranes with 5% non fat dry milk in PBS- Tween (0, 01%) were incubated over-night with specific primary antibodies. The immunoreactive protein bands were detected by incubation with horseradish peroxidase-conjugated secondary goat anti rabbit (Millipore) or goat anti mouse (Sigma-Aldrich) antibodies. The western blot images were acquired on a ImageQuant LAS 4000 (GEHC) and quantified by ImageJ software.
The following antibodies were purchased from Santa Cruz Biotechnology: myogenin (sc-576) cyclin D1 (sc-718), TrxR1 (sc-20147), β-actin (sc-47778), MyHC (sc-20641) and p21(sc-397). GAPDH (MAB 374) antibody was purchased from Millipore.

Mercatelli N., Fittipaldi S., De Paola E., Dimauro I., Paronetto M.P., Jackson M.J, & Caporossi D. (2017). MiR-23-TrxR1 as a novel molecular axis in skeletal muscle differentiation. Scientific Reports, 7, 7219.

+ Open protocol

+ Expand

Immunoblotting of Oncogenic Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded in 6-well plates and treated with indicated agents for specified timepoints. Lysates were prepared as previously described, and equal volumes of total cell lysate were processed for immunoblotting10 . Antibodies against phospho-ALK Y1282/1283 (9687), ALK (3633), phospho-AKT S473 (4060), AKT (4691), Phospho-ERK T202/Y204 (9101), ERK (9102), Phospho-Paxillin Y118 (2541), SHP2 (Cell Signaling 3752), and EGFR (4267), were obtained from Cell Signaling Technology and used at 1:1000 dilution. Antibodies against Phospho-EGFR Tyr1068 (AB5644) and Phospho-RSK 359/S363 (AB32413) were purchased from Abcam and used at 1:1000 dilution. GAPDH (MAB374) antibody was purchased from Millipore and used at 1:5000 dilution. K-RAS (F234), H-RAS (F235) and N-RAS (F155) antibodies were purchased from Santa Cruz and used at 1:500 dilution. All secondary antibodies (anti-mouse IgG HRP-linked (7076S) and anti-rabbit IgG HRP-linked (7074S)) were purchased from Cell Signaling and used at 1:50000 dilution.

Dardaei L., Wang H.Q., Singh M., Fordjour P., Shaw K.X., Yoda S., Kerr G., Yu K., Liang J., Cao Y., Chen Y., Lawrence M.S., Langenbucher A., Gainor J.F., Friboulet L., Dagogo-Jack I., Myers D.T., Labrot E., Ruddy D., Parks M., Lee D., DiCecca R.H., Moody S., Hao H., Mohseni M., LaMarche M., Williams J., Hoffmaster K., Caponigro G., Shaw A.T., Hata A.N., Benes C.H., Li F, & Engelman J.A. (2018). SHP2 inhibition restores sensitivity to ALK inhibitors in resistant ALK-rearranged non-small cell lung cancer. Nature medicine, 24(4), 512-517.

+ Open protocol

+ Expand

Angiopoietin-2 Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant mouse Angpt2 proteins (7186-AN), anti-Mouse/Rat Tie2 antibody (AF762), and control polyclonal Goat immunoglobulin G (IgG) (AB-108-C) were purchased from R&D Systems (USA). Antibodies against STAT3 (9139), phospho-STAT3 (Tyr705) (9131), JAK1 (3332), phospho-JAK1 (Tyr1034/1035) (3331S), Bcl-XL (2762), and Bcl-2 (3498); Annexin/PI kits (6592) and caspase-3 activity assay kit (5723); and anti-rabbit (7074S)/mouse (7076S) IgG horseradish peroxidase (HRP)-linked antibody were obtained from Cell Signaling Technology (USA). Caspase-9 activity assay kit (K119) was purchased from BioVision Technologies (USA). GAPDH antibody (MAB374) was obtained from Millipore. SOCS5 antibody (PA1-41253) was obtained from Thermo Fisher Scientific (USA).

Tsai Y.C., Kuo P.L., Hung W.W., Wu L.Y., Wu P.H., Chang W.A., Kuo M.C, & Hsu Y.L. (2018). Angpt2 Induces Mesangial Cell Apoptosis through the MicroRNA-33-5p-SOCS5 Loop in Diabetic Nephropathy. Molecular Therapy. Nucleic Acids, 13, 543-555.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein samples for western blot analysis were prepared in the same way as for ELISA. Protein samples (30 μg) were separated on 10% SDS-PAGE gels and transferred to PVDF membranes. The membranes were blocked with 5% BSA for 2 h at RT to avoid non-speci c binding. Following incubation with antibodies, such as p-ERK (9101, rabbit, 1:1000, Cell Signaling, Boston, USA), p-JNK (4688, rabbit, 1:1000, Cell signaling, Boston, USA), p-NF-κB (3033, rabbit, 1:1000, Cell signaling, Boston, USA), or GAPDH antibody (MAB374, mouse, 1:10,000, Millipore, Billerica, MA, USA), at 4°C overnight, the membranes were further incubated with IRDye 800CW antibody for 2 h in the dark at RT. Images were captured using the Odyssey Imaging System (LI-COR Bioscience, Lincoln, NE), and grayscale values were analyzed using Image J software (NIH, Bethesda, MD, USA).

Xia A., Huang H., You W., Liu Y., Wu H., & Liu S. (2020). The neuroprotection of hyperbaric oxygen therapy against traumatic brain injury via NF-κB/MAPKs-CXCL1 signaling pathway.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Xia A., Huang H., You W., Liu Y., Wu H, & Liu S. (2022). The neuroprotection of hyperbaric oxygen therapy against traumatic brain injury via NF-κB/MAPKs-CXCL1 signaling pathways. Experimental brain research, 240(1).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!