Lh elisa kit

Manufactured by MyBioSource

Sourced in United States

The LH ELISA Kit is a laboratory equipment designed to quantitatively measure the concentration of Luteinizing Hormone (LH) in biological samples, such as serum or plasma. The kit utilizes an Enzyme-Linked Immunosorbent Assay (ELISA) method to detect and quantify the LH levels.

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Lab products found in correlation

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ELISAs (6 citations)

4 protocols using lh elisa kit

Quantifying Reproductive Hormones

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To evaluate hormone levels, we collected peripheral blood and conducted a competitive-based enzyme-linked immunosorbent assay (ELISA) using an estrogen ELISA kit (R&D Systems,
Minneapolis, MN, USA), a mouse FSH ELISA kit, and an LH ELISA Kit (both from MyBiosource, San Diego, CA, USA). Absorbance at 450 nm was measured using a SpectraMax ABS device (Molecular
Devices, San Jose, CA, USA). Data are expressed as pg of estrogen per ml of serum and ng of FSH or LH per ml of serum.

KANATSU-SHINOHARA M., LEE J., MIYAZAKI T., MORIMOTO H, & SHINOHARA T. (2022). Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice. The Journal of Reproduction and Development, 68(6), 369-376.

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Serum Lipid and Hormone Profiling

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Blood serum was obtained by centrifugation (500 g for 10 min at 4°C) and stored at -20°C until being used to assess blood lipid profiles (total cholesterol (TC), high density lipoprotein (HDL), low/very low density lipoprotein (LDL/VLDL) and cholesterol) using a commercially available ELISA assay kit (ab65390, ABCAM, U.K.) according to the manufacturer’s directions.
The serum testosterone, FSH and LH levels were assessed using the total antioxidant capacity kit (ab65329, Abcam, United Kingdom), testosterone ELISA kit (ab108666, Abcam, United Kingdom), FSH ELISA kit (MBS703380, MyBioSource, U.S.A) and LH ELISA kit (MBS041300, MyBioSource, U.S.A), respectively (according to the manufacturers protocols).

Dardmeh F., Alipour H., Gazerani P., van der Horst G., Brandsborg E, & Nielsen H.I. (2017). Lactobacillus rhamnosus PB01 (DSM 14870) supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model. PLoS ONE, 12(10), e0185964.

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Measuring Reproductive Hormones in Mice

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The levels of estrogen, LH, and FSH in the serum of mice were measured by a competitive-based enzyme-linked immunosorbent assay (ELISA) using an estrogen ELISA kit (R & D systems, Minneapolis, MN), an LH ELISA Kit, and a mouse FSH ELISA kit (both from MyBiosource, San Diego, CA). Absorbance at a wavelength of 450-nm was measured using an automated microplate reader. Data were expressed as pg of estrogen per mL of serum, and ng of LH and FSH per mL of serum.

Kanatsu-Shinohara M., Lee J., Miyazaki T., Morimoto H, & Shinohara T. (2022). Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice. Cell Reports Medicine, 3(5), 100606.

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Plasma LH Concentration Determination

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Blood was collected from the jugular vein in a 50 ml falcon tube together with 20 μl 0.5 M EDTA immediately after decapitation. 2,0 ml of blood was further transferred to an eppendorf tube and centrifuged at 3000 rpm at room temperature for 20 min. Plasma was stored at -20 °C until further analysis.
The concentration of luteinizing hormone in the plasma samples was determined using a commercial LH ELISA kit (MyBiosource, San Diego, USA), according to the manufacturer's instructions. All samples were tested in duplicate and the analytic range of the assay was 6.25-200 ng/ml.

Karlsson A.C., Fallahshahroudi A., Johnsen H., Hagenblad J., Wright D., Andersson L, & Jensen P. (2016). A domestication related mutation in the thyroid stimulating hormone receptor gene (TSHR) modulates photoperiodic response and reproduction in chickens. General and comparative endocrinology, 228.

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